Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"episomes"”.
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The genetics of glucosamine in yeast : cytoplasmic determinants conferring resistance
… 8. unlike yeast carrying resistance conferring episomes GR6 and GR10 were not resistant to venturicidin or oligomycin and the GR factor exhibited genetic behaviour different from that of the episomal determinants. These results 7--+8 suggested that glucosamine resistance was not associated with …
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Investigations into the use of maize streak virus as a gene vector
… A high proportion of these contained MSV-based episomes at high copy number. However, embryogenic maize tissue of the Hill line was not regenerable when an MSV-based replicon was present, possibly due to toxicity of the viral replication associated protein. In non-regenerable Black Mexican …
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Development of Extrachromosomal Genetic Technologies for Persistent and Tunable Expression of Therapeutic Payloads
… by latent DNA viruses with genomes persisting as episomes, we developed an extrachromosomal genetic technology (EGT). Our EGT, featuring an engineered origin of replication and tether protein, maintained non-integrated transgene expression in proliferative cells over 12 weeks. Despite this …
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Development and Characterization of Viral-Based Gene Editing In Vivo
… insertion and deletion mutations into AAV episomes, understanding the effects AAV-CRISPR editing in vivo in a Cas9 immunized mouse model, and the characterization of AAV integrations into the genome in the context of CRISPR-based gene editing.
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Creating a versatile toolkit for transgene expression using BACS and for dissecting large-scale chromatin organization
… of multiple transgenes maintained either as episomes or stably integrated copies. Moreover, in Chapter 3 we show that BACs could be used as a powerful model system for dissecting mechanisms regulating large-scale chromatin organization, by demonstrating distinctive large-scale chromatin …
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OPTIMIZING GENE THERAPY: THE IMPACT OF SMALL MOLECULE MODULATORS ON ADENO-ASSOCIATED VECTOR TRANSDUCTION AND INTEGRATION
… Recombinant AAV genomes primarily remain in episomes, but AAV integration events occur at a low frequency. These events can be genotoxic. Therefore, studies to investigate the impact of modulators on AAV transduction should be coupled with characterization of AAV integration. Here, we profile …