Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 43 for “"epigenetic modification"”.
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Epigenetic Modification of Phenotype in Intestinal Smooth Muscle Cells
… In other diseases of smooth muscle hyperplasia, epigenetic modifications, including DNA methylation and histone acetylation, affect smooth muscle phenotype and contribute to impaired tissue function. Any epigenetic role in regulating gene expression and the contractile phenotype of ISMC is …
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Hypothalamic mechanisms of appetite regulation involve stress response and epigenetic modification
… occur during critical periods of life, influence epigenetic programming and gene expression in the long-term. Therefore, the aim of this dissertation was to elucidate how hypothalamic mechanisms of appetite regulation correlate with the stress response and epigenetic modifications, using avian …
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Epigenetic Modification as a Therapeutic Target in BRAFV600E-mutated Metastatic Colorectal Cancer
… this is a unique subgroup harboring aberrant epigenetic phenotype. Firstly, DNA methyltransferase (DNMT) inhibitor treatment induced profound DNA hypomethylation in vivo, but minimal change in gene expression due to adaptive elevation of the repressive histone methylation, H3K27me3, leading to …
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Functional consequences of cytosine methylation in mitochondrial DNA catalyzed by DNA methyltransferase 1
… ago, but neither the mechanism generating this modification nor its functional significance was known. Because mitochondrial dysfunction is a hallmark characteristic of numerous human diseases, including neurological and cardiovascular disease, aging and cancer, this dissertation addressed …
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CHD Chromatin Remodelers Promote Epigenetic Control of Development in Arabidopsis thaliana
… family of chromatin remodelers play key roles as epigenetic modifiers and transcriptional regulators in both plants and animals. PKL is a CHD3 remodeler that represses expression of seed genes during germination in Arabidopsis. Unlike animal CHD3 remodelers, PKL does not function as a member of a …
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CHARACTERIZATION OF DNA METHYLATION PATTERNS IN HUMAN EMBRYONIC STEM CELLS AND COLORECTAL CANCER USING WHOLE GENOME BISULFITE SEQUENCING
DNA methylation is an epigenetic modification essential for normal development. Inappropriate regulation of DNA methylation can cause abnormal silencing of tumor suppressor genes leading to cancer development. Despite the importance of this mechanism in human health, our existing knowledge is …
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Biomechanical regulation of epigenetics and chromatin organization in single living cells
… decision-making. These mechanisms include epigenetic modification, chromatin organization, and gene expression. It is, therefore, highly important to investigate force-induced regulation of these mechanisms to better understand human physiology and disease. In this research, we aim at …
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Functional genomics of developmental disorders
DNA methylation, or the epigenetic modification of primarily cytosine bases within DNA to 5-methylcytosine through the addition of a methyl group, is an epigenetic mark with a variety of biological and cellular roles. Genetic and environmental influences can perturb DNA methylation patterns in …
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A sequencing-based analyisis of epigenetic modifications of the mitochondrial genome
… on CpG residues is one of the most important epigenetic modifications of nuclear DNA, where it regulates gene expression. Methylation of mitochondrial DNA (mtDNA) has been studied using whole genome bisulfite sequencing (WGBS), but recent evidence has uncovered major technical issues, which …
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Creation of Versatile Cloning Platforms for Transgene Expression and Epigenome Editing and Their Application to Pancreatic Islet Biology
… foundation, we also developed an S. aureus dCas9 epigenetic engineering platform, pMAGIC, that enables the packaging of 3 guide RNAs with Sa-dCas9 fused to one of five epigenetic modifiers into a single vector. Using pMAGIC-derived adenoviruses, we functionally validated the regulation of PDX1 by …
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Effects of m6A DNA methylation by bacterial methyltransferase in colorectal cancer
… cancer progression. Evidence for endogenous m6A modification in human DNA is limited and controversial so the functional impact of m6A DNA modification remains uncertain. We tested our hypothesis by ectopically expressing M.FnI in HCT116 colorectal cancer cells using expression constructs that …
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A functional data analytic approach for region level differential DNA methylation detection
"DNA methylation is an epigenetic modification that can alter gene expression without a DNA sequence change. The role of DNA methylation in biological processes and human health is important to understand, with many studies identifying associations between specific methylation patterns and diseases …
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AN ANALYSIS OF THE DIFFERENTIAL METHYLATION AND EXPRESSION OF IMPRINTED GENES IN M. M. MUSCULUS, M. M. DOMESTICUS, AND THEIR HYBRIDS
<p>Epigenetics has been found to have an effect on many aspects of biology. Epigenetics refers to modifications of the double-stranded DNA molecule, which do not change the nucleotide sequence but do affect gene expression. DNA methylation is a type of epigenetic modification. Genomic imprinting is …
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Application of High-Performance Liquid-Chromatography Mass-Spectrometry Platform to Study Metabolism and Epigenetic Control of Metabolism
… effect. We hypothesize that cancer metabolism is epigenetically regulated. Epigenetics refers to inheritable traits that are not due to alterations in the primary DNA sequence. DNA methylation is an important epigenetic modification. DNA methylation mainly occurs in the CpG islands of the promoter …
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Zika Modulates Arthropod Histone Methylation in Mosquito Cells
<p>Epigenetics is the heritable series of covalent modifications that affect chromatin structure, gene expression, and protein function. Methylation is one such epigenetic modification that involves the addition of chemical modifying entities, such as methyl groups, on nucleic acids or proteins. …
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Propranolol Elicits Long Term Systemic Effects After Repetitive Mild Traumatic Brain Injury
… important for metabolism, cytokine signaling, epigenetic modification, innate, and adaptive immunity. We also find that propranolol reduces the presence of Ly6C+ and increases the presence of Ly6C- monocytes in the blood one month after injury; however, it leads to increased Ly6C+ monocyte …
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Identification of host cellular factors that regulate human norovirus replication
… for type III interferons is suppressed by epigenetic modification of its promoter, and cells lacking the receptor were more permissive for HuNoV replication. Secondly, the role of the heat shock protein 90 (Hsp90) on HuNoV replication was examined. While this molecular chaperone was …
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The Regulation of Dna Methylation In Mammalian Development and Cancer
<p>DNA methylation is an essential epigenetic modification in mammals, as it plays important regulatory roles in multiple biological processes, such as gene transcription, maintenance of chromosomal structure and genomic stability, genomic imprinting, retrotransposon silencing, and X-chromosome …
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Genomics and epigenomics using 2D nanopores
DNA methylation is an epigenetic modification caused by the addition of a methyl group to DNA and heavily involved in gene expression and regulation, thereby critical to the progression of diseases such as cancer. In this work we show that detection and localization of DNA methylation can be …
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Technology development in mouse genetics and epigenetics
… stem cells and make mice that carried genetic modification by zygotic injections. Using CRISPR/Cas9 we were able to make mice in as little as three weeks that contained multiple gene knockouts, single nucleotide modifications, GFP and mCherry reporter alleles, epitope-tagged alleles, and …
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