Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"enzyme structure"”.
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Enzyme structure, function, and evolution in flavonoid biosynthesis
… million years. This dissertation examines the structure, function, and evolution of enzymes in the flavonoid biosynthetic pathway. First, we structurally and biochemically characterized orthologs of chalcone synthase (CHS), the enzyme that catalyzes the first step of flavonoid biosynthesis, …
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A Biochemical Study of Gossypol and Lactate Dehydrogenase X Binary Interactions
… indicators of intramolecular disturbance in the enzyme molecule. Definite alterations in peptide fragment peaks were observed in the presence of gossypol and suggest conformational or intramolecular modifications in the enzyme structure attributable to direct binding with gossypol or its …
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Site-specific protein labeling via sortase A and its applications/
… labeling method has been implemented to explore enzyme structure and function, improve the physical properties of therapeutic proteins, study glycoproteins important for innate immune responses in living cells (Appendix A), and visualize influenza glycoproteins in living, infected cells. Finally, …
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Side chain interactions at the dimer interface of Vibrio alkaline phosphatase determine its reactivity, stability and desthiobiotin inhibition
… (VAP) is a homodimeric, psychrophilic enzyme. A homodimer is, by far, the most dominant quaternary enzyme structure found in nature. It is therefore obvious that this particular structure gives the enzymes some advantage in the evolutionary race. Many suggestions have been proposed, …
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Statistical and Relational Learning for Understanding Enzyme Function
… in living systems is a challenging task. Enzymes are involved in almost all of the chemical processes taking place within the cell. They accelerate chemical reactions by forming a complex with the substrate and therefore lowering the reaction activation energy. The characterisation of the …
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Characterization of the Bacteriophage Felix O1 Endolysin and Potential Application for Salmonella Bioremediation
… characterized. PHYRE analysis predicted that the enzyme structure is composed of α helices arranged into two lobes, with the active site in a cleft between them. The enzyme lysed all tested strains of Salmonella and a tested strain of the foodborne pathogen Escherichia coli. Campylobacter jejuni …
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Rationalising and accelerating enzyme engineering by quantitative sequence-function profiling in microdroplets
Enzymes catalyse nearly all reactions in Nature's vast chemical repertoire with high precision and under sustainable conditions. To apply them in cost-effective and sustainable biocatalytic production processes in chemical industry, enzymes first need to be modified or "engineered" to work with …
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THE BIOTRANSFORMATION OF BILE ACIDS BY A COMMON MEMBER OF THE BISON GUT MICROBIOME
… kinetic characterization of any member of this enzyme family with a pan-family inhibitor. BSHAc showed optimum activity at ~57 °C and between pH 5 and 6. Steady-state kinetic characterization of BSHAc using glycine and taurine conjugates of cholic (CA) and deoxycholic acid (DCA) revealed it was …
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COMPUTATIONAL ENZYME ENGINEERING: FROM MOLECULAR MODELING TO MACHINE LEARNING
… of biocatalysis in modern life sciences. Enzymes, as highly selective and efficient natural catalysts, offer significant advantages for environmentally benign and carbon-neutral processes. However, their practical application is often limited by narrow substrate scope, insufficient …
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Assembly of cytochrome c oxidase: the role of hSco1p and hSco2p
… is not surprising given the complexity of the enzyme structure and the multiple factors and many steps required for its assembly. A functional COX requires three mitochondrially encoded subunits (Cox1p, Cox2p and Cox3p), at least 10 nuclearly encoded subunits, some of which are tissue specific, …
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Molecular Genetics and Subcellular Localization of Flavonoid Metabolism in Arabidopsis
There are at least two models describing how the enzymes of metabolic pathways are arranged in living cells. The first is a stochastic model, where enzymes are freely-diffusing in the aqueous environment of the cell, and the second, the metabolon model, has pathway enzymes organized as enzyme …
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Target Based Design and Synthesis of Fused Pyrimidines in the Potential Treatment of Cancer and Opportunistic Infection
… expressing mutated dihydropteroate synthase enzyme (target of SMX). For patients unresponsive or resistant to this treatment, newer drugs are critically needed. The absence of an X-ray crystal structure of pjDHFR poses a large gap in drug discovery efforts. The status quo, as it pertains to …
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Structural and biochemical studies on enzymes involved in natural product biosynthesis and cellular respiration
… focuses on structural studies of three enzymes involved with natural product synthesis. The most widely distributed component of brown algae is alginate, a cell wall polysaccharide composed of polymeric blocks of α-(1,4) O-linked β-D-mannuronate (M) and its C-5 epimer α-L-guluronate (G). …
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ΕΠΙΔΡΑΣΗ ΤΩΝ ΑΜΙΝΩΝ ΣΤΙΣ ΔΟΜΙΚΕΣ ΚΑΙ ΚΑΤΑΛΥΤΙΚΕΣ ΙΔΙΟΤΗΤΕΣ ΤΗΣ ΓΛΥΚΟΓΟΝΙΚΗΣ ΦΩΣΦΟΡΥΛΑΣΗΣ Β
… OF AMP INDUCE A SIGNIFICANT ENHANCEMENT OF ENZYME ACTIVITY. THE BINDING CENTER OF PHOSPHORYLASE B FOR AMINES IS POSSIBLY LOCATED ON THE N-TERMINAL CHAIN OF PHOSPHORYLASE B. FROM ALL AMINES, POLYMYXIN B IS THE MOST EFFECTIVE IN STIMULATING AMP DEPENDENT ACTIVITY OF ENZYME.