Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"enzyme mechanism"”.
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Mechanistic studies of lanthipeptide biosynthesis
… study of CylM provides further insights into the mechanism of the modification process. The maturation of lanthipeptides typically requires a proteolytic step that removes the leader peptide from the modified precursor peptides. Characterization of two peptidases involved in the synthesis of …
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Biochemical Characterization of Thermocrispum agreste TheA: A Flavin-Dependent N-hydroxylating Enzyme
… found only in fungi and bacteria. These enzymes catalyze the hydroxylation of nucleophilic primary amines, such as those found in histamine, L-ornithine, L-lysine, and small aliphatic diamines. The hydroxamate moiety produced by this reaction is key for the production of siderophores, …
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Insights into rotaxane formation enable cyclase engineering for lasso peptide diversification
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01
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STUDIES OF THE ELECTROSTATIC PROPERTIES OF PEROXIREDOXINS
… completes the reaction cycle. The particular enzyme mechanism classifies the whole Prxs family of proteins into 1-Cys, typical 2-Cys, and atypical 2-Cys Prx subfamilies. Typical 2-Cys Prxs are obligate homodimers, and usually form decamers in solution. Because the molecular function of Prxs …
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A Specialized Citric Acid Cycle Requiring Succinyl-Coenzyme A (CoA):Acetate CoA-Transferase (AarC) Confers Acetic Acid Resistance on the Acidophile Acetobacter aceti
… the combined contributions of several molecular mechanisms. This study examines the inherent acid stability of proteins from the industrial vinegar-production strain Acetobacter aceti 1023 and the process by which cytoplasmic acetic acid is "overoxidized" to carbon dioxide. Acetate overoxidation …
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Structural and mechanistic studies on eukaryotic UDP-galactopyranose mutases
… for drug design. Galf is synthesized by the enzyme UDP-galactopyranose mutase (UGM), which converts UDP-galactopyranose (UDP-Galp) to UDP-galactofuranose (UDP-Galf). Solving the mechanism and structure of UGMs will aid in the development of specific inhibitors against these enzymes. Herein we …
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An Investigation of Acetobacter aceti N5-Carboxyaminoimidazole Ribonucleotide Mutase and Its purE-purK Operon
… metabolite has been selected for study. The enzyme N5-carboxyaminoimidazole ribonucleotide mutase: PurE) catalyzes the reversible intramolecular transfer of the carboxylate of N5-carboxyaminoimidazole ribonucleotide: N5-CAIR) to the C-4 position of the imidazole ring to form …
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Enzymatic Characterization of N-Acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside Deacetylase (MshB)
… MshB employs a general acid-base catalyst mechanism wherein the Asp15 functions as a general base to activate the metal-bound water nucleophile for attack of the carbonyl carbon on substrate. Proton-transfer from a general acid catalyst facilitates breakdown of the tetrahedral intermediate …
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Rationalising and accelerating enzyme engineering by quantitative sequence-function profiling in microdroplets
Enzymes catalyse nearly all reactions in Nature's vast chemical repertoire with high precision and under sustainable conditions. To apply them in cost-effective and sustainable biocatalytic production processes in chemical industry, enzymes first need to be modified or "engineered" to work with …
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Structural and Mechanistic Studies on N-Hydroxylating Monooxygenases Involved in Siderophore Biosynthesis
… monooxygenases (NMOs) are flavin dependent enzymes that primarily catalyze the hydroxylation of L-ornithine or L-lysine. This is the first, committed step to siderophore biosynthesis. Pathogenic microbes including Aspergillus fumigatus and Mycobacterium tuberculosis secrete these low …
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Interplay of Transition Metals and Noncovalent Interactions in C–H Activation Catalysis
… biological systems, such as non-heme iron enzymes, carry out selective C–H activation efficiently under ambient conditions. These enzymes catalyze a variety of reactions including C–H halogenation, hydroxylation, epoxidation, and ring closures, several of which are mediated with the help of …
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TRIM21 Mediated Antibody-Dependent Intracellular Immunity
… almost exclusively on the extracellular effector mechanisms of antibodies such as opsonisation, complement activation, antibody-dependent cytotoxicity and receptor blocking. The discovery of TRIM21 as a cytosolic antibody receptor has revolutionised our understanding of humoral immunity and …
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Substituted aryl glycosides as probes of the mechanism of spontaneous ad enzyme-catalyzed glycoside hydrolysis
… was synthesized and used to probe the mechanisms of spontaneous and enzyme-catalyzed hydrolysis of 0-glycosides. The relative rates of spontaneous hydrolysis of the DNP glucosides followed the trend 4-deoxy > 3-deoxy - 6-deoxy > parent > 6-deoxy-6-fluoro > 3-deoxy-3-fluoro > …
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Mechanistic Studies of Flavin-Dependent Monooxygenases Involved in Bacterial Defense and Plant Metabolism
… monooxygenases (FMOs) are a large family of enzymes found in microbes, plants, animals, and humans involved in defense pathways, xenobiotic metabolism, and natural product biosynthesis. One class of FMOs, Class B, carries out the oxidation of heteroatomic substrates, via hydroxylation, …
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Mechanisms of Flavin-Dependent Monooxygenases Involved in Natural Product Chemistry
… deaths in 2019 alone. One approach is utilizing enzymes to synthesize existing natural products to improve the yields and stereospecificity issue. This dissertation is focused on the biochemical characterization of three enzymes-ZvFMO, OxaD, and CreE-that are implicated in the detoxification of …
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Sortases: Keystones to Virulence and Targets for Anti-Infective Therapy
… chemotherapeutics is the sortase class of enzymes. Sortases are extracellular transpeptidases unique to Gram-positive bacteria. Their function is to covalently attach secreted virulence factors to the bacterial cell wall. Deletion or inhibition of sortases results in severe attenuation of …
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Post-translational regulation of BiP by FICD-mediated AMPylation and deAMPylation
… protein load this bifunctional (monomeric) Fic enzyme AMPylates and inactivates excess ATP-bound BiP. However, with increasing ER stress dimeric FICD rapidly deAMPylates the inactive BiP-AMP store — enabling extra BiP to re-enter the chaperone cycle and thereby increase the organelle’s chaperone …
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Structure-Based Drug Design Against a Biosecurity Pathogen
… an allosteric site. We show that binders inhibit enzyme activity, with promise for further development. Lastly, using cryo-EM we determined the structure of the C. burnetii ribosome (the target of doxycycline), revealing CLaSP, a previously unannotated gene encoding a large subunit peptide and a …