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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 74 for “"enzyme kinetics"”.
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Enzyme Kinetics of Recombinant Human Arylsulfatase B (rhASB)
<p>This project seeks to explain the enzyme kinetics of recombinant human Arylsulfate sulfatase B (rhASB) by using an in vitro bioassay and direct hydrolysis substrate assay to approach the problem of substrate limitation in establishing a representative kcat for rhASB. The project also explores …
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Bacterial interactions and enzyme kinetics in environmental polyester degradation
… PBSeT enrichment experiments. Monoculture Monod kinetics on the monomers of the polymer revealed each bacterium’s preference and competitiveness for PBSeT substrates. Subsequently, coculture incubations with the whole plastic revealed how these dynamics manifest in complex systems. Informed by …
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Raman Chemometrics and Application to Enzyme Kinetics and Urinalysis
… in novel areas. Fast and accurate evaluation of enzyme specific activity is required for engineering enzymes, and results of Raman assays, evaluated in the RDA Toolbox, are successfully correlated to absorbance activity assays of an enzyme WT and mutant library. Further development of this …
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Studies of Enzyme Kinetics and Inhibition Through Capillary Electrophoretic Enzyme Assays
Enzyme assays are used throughout the biological, chemical, and medical sciences for the study of enzymes, and typically are performed by bulk solution measurements in cuvettes or microplates. The development of new enzyme assays that combine the measurement of reaction rates with separation …
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Frequency-domain enzyme kinetics in the context of artificial calcium oscillations
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1996.
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Single-Enzyme Kinetics with Fluorogenic Substrates: Strategies Towards Accurate Single-Turnover Detection
Contains fulltext : 161454.pdf (Publisher’s version ) (Open Access)
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The evaluation of the specificity of the Acid Phosphatase test to identify semen
… (AP) by a colour change reaction. AP is an enzyme which is present in human semen in high concentrations, but is also found in animal semen at lower concentrations. The current methods of presumptive testing cannot differentiate between animal and human semen. The specificity of the …
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Orally Administered IGF -I Combined With Partial Enteral Nutrition Alters Intestinal Morphology and Disaccharidase Activity During Parenteral Nutrition in Neonatal Piglets
… LPH degradation or specifically altering LPH enzyme kinetics.
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Characterization of Moraxella bovis Aspartate Transcarbamoylase
… B, and C, based on their molecular weight, holoenzyme architecture, and enzyme kinetics. Moraxella bovis is a fastidious organism, the etiologic agent of infectious bovine keratoconjunctivitis (IBK). The M. bovis ATCase was purified and characterized for the first time. It is a class A enzyme …
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Modeling of nucleation-based stochastic processes in cellular systems
… in DNA transcription, and single-molecule enzyme kinetics. We focus on the key experimental results and build up stochastic models accordingly to provide quantitative insights to the underlying physical mechanisms for the corresponding biological processes. We utilize specific mathematical …
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EUKARYO: An Agent-Based, Interactive, Virtual Reality Simulation of a Eukaryotic Cell
… mathematical modelling towards modelling enzyme kinetics, and agent-based modelling to illustrate the interaction between the individual proteins in the cell. Using hybrid modelling, Eukaryo is able to model the interactions for a diverse range of biological processes, such as protein …
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Kinetics of Acid Inactivation of Soybean Lipoxygenase and Its Effects on the Functional Properties of Soy Protein
… whole soybeans in water acidified with HCl. The enzyme was found to be completely and irreversibly inactivated at pH 3.0 and below. From a study of enzyme kinetics of lipoxygenase extracted from the acidified soy slurries, significant changes were observed with the values of the Michaelis …
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Mechanistic insight on a chimeric Cas9 protein's specificity for DNA target with 5 '-NAA-3' PAM
… practical use. Here, we use of the principles of enzyme kinetics to investigate our chimeric protein's comparative efficiency to Cas12a and the biophysical mechanism by which our grafted S. macacae segment works synergistically with the S. pyogenes Cas9 scaffold to cleave target DNA with an NAA …
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Acoustically-levitated drop reactor (ldr) employable for kinetics measurements of biochemical networks
Accurate measurement of enzyme kinetics is an essential part of understanding the mechanisms of biochemical reactions. The typical means of studying such systems use stirred cuvettes, stopped-flow apparatus, microfluidic systems, or other small sample containers. These reaction-kinetics …
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Biochemical and Cellular Characterization of NMN Adenylyltransferase 2: A Brain Specific Isoform of an Essential NAD Synthesizing Enzyme
… 2 (Nmnat2) is one of three vertebrate enzymes that catalyze the synthesis of NAD from NMN and ATP. Nmnat1, -2 and -3 are each expressed from a separate gene and are distinguished from one another by their expression, subcellular localization and enzyme kinetics. Nmnat overexpression in …
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Towards a paradigm shift in the modeling of soil organic carbon decomposition for earth system models
… account for microbial biomass pools and enzyme kinetics that catalyze soil carbon decomposition produce notably different results and provide a closer match to recent observations. However, a systematic evaluation of the advantages and disadvantages of the microbial models and how they …
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ΛΕΙΤΟΥΡΓΙΚΟΤΗΤΑ ΤΟΥ ΑΓΓΕΙΑΚΟΥ ΠΝΕΥΜΟΝΙΚΟΥ ΕΝΔΟΘΗΛΙΟΥ, IN VIVO, ΥΠΟ ΦΥΣΙΟΛΟΓΙΚΕΣ ΚΑΙ ΠΑΘΟΛΟΓΙΚΕΣ ΣΥΝΘΗΚΕΣ
… DELIVERY TO THE CHEST, ENDOTHELIAL-ASSOCIATED-ENZYME ACTIVITIES CAN BE IMPAIRED. WE INVESTIGATED THE EARLY EFFECTS OFIONIZING RADIATION ON PULMONARY ENDOTHELIAL FUNCTIONS IN RABBITS EXPOSED TO A SINGLE DOSE (30 GY) OF IONIZING RADIATION TO THE CHEST, WITH OR WITHOUT POST-RADIATION INDOMETHACIN …
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Mass fluctuation kinetics : analysis and computation of equilibria and local dynamics
The mass fluctuation kinetics (MFK) model is a set of coupled first-order differential equations describing the temporal evolution of means, variances and covariances of species concentrations in systems of chemical reactions. It generalizes classical mass action kinetics (MAK) in which …
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Tissue enzyme changes in air-exposed American eels.
… thesis will investigate the changes in tissue enzymes of the American eel as a result of air exposure. It is the hypothesis of this experiment that conditions of air exposure eels will undergo a metabolic reorganization including an initial Pasteur effect and metabolic depression indicated by …
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Investigating the Biochemical and Catalytic Properties of Nitrile Hydratases
… (NHase, E.C. 4.2.1.84). NHase is a metalloenzyme that efficiently converts nitriles to amides at neutral pH and ambient temperatures, thus reducing production of unwanted by-products and toxic wastes. NHase contains either a non-heme Fe<super>3+</super> or a non-corrin Co <super>3+</super> …
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