Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"enzyme immobilization"”.
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Bio-Nano Interfaces: Enzyme Immobilization for Biomimetic Energy Harvesting
… of protein/DNA assembly. The specificity of the enzyme immobilization was controlled by small protein structural motifs, called zinc fingers (ZnF) that bind to specific dsDNA sequences and may be genetically bound to small laccase (SLAC-3ZnF) or other redox enzymes. Utilizing scaffolds with gold …
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Emulsion-Templated Porous Polymers as Support Materials for Covalent Enzyme Immobilization
… can be used as a biocatalysts, via the covalent immobilization of Candida Antarctica Lipase B (CAL-B). Recently, it has been shown that polyHIPEs can be prepared with epoxy functionality, which show potential for the covalent immobilization of enzymes. The aims of our work were, firstly, to …
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Studies of the Preparations and Use of Sol-Gel for Enzyme Immobilization and Analytical Applications.
<p>Much attention has been paid to immobilization of enzymes to improve enzyme stability and permit its reuse. Glucose oxidase entrapment in different kinds of silica sol-gel matrices was investigated. The enzyme showed stable activity for 11 uses in the sol-gel with tetramethyl orthosilicate …
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Estudo da copolimerização de estireno e cardanol em meios homogêneo e heterogêneo
… and cardanol is promising as support for enzyme immobilization. Thus, this dissertation aims to study the kinetic behavior of cardanol and styrene copolymerization, through experiments using different polymerizations techniques and mathematical model developed to describe experimental …
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OPPORTUNITIES AND CHALLENGES OF BIOCATALYSIS IN PHARMACEUTICAL SCIENCES: DEVELOPING TAILORED BIOCATALYSTS FOR SYNTHETIC APPLICATIONS
… To overcome typical limitations of wild-type enzymes, such as insufficient operational stability and narrow substrate scope, two complementary strategies were applied: enzyme immobilization and protein engineering. First, a biocatalytic cascade combining the ThDP-dependent enzyme Ao:DCPIP OR …
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A systematic study of enzyme-nanomaterial interactions for application in active surface decontamination
Enzymes are a catalytic class of proteins that possess high specificity, selectivity and biocompatibility, which makes them ideal for multiple applications in industrial production and biotechnology. However, the use of enzymes in such applications is limited due to their low operational stability …
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Investigating the Biochemical and Catalytic Properties of Nitrile Hydratases
… (NHase, E.C. 4.2.1.84). NHase is a metalloenzyme that efficiently converts nitriles to amides at neutral pH and ambient temperatures, thus reducing production of unwanted by-products and toxic wastes. NHase contains either a non-heme Fe<super>3+</super> or a non-corrin Co <super>3+</super> …
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Tailoring Microenvironment and Orientation of Immobilized Lactase for Improved Catalysis at Suboptimal pH
… has emerged as a promising solution. Covalent immobilization allows enzymes to be reused and prevents contamination of the product. While immobilizing lactases has been found to enhance their pH and temperature stability, undesired enzyme-substrate interactions can still lead to reduced enzyme …
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Enzymatic Electrode Design: A Systematic Approach to Enhanced Bioelectrocatalysis
… key design parameters is concerned with how the enzyme catalyst engages the underlying electrode current collector. This one design consideration has led to considerable branching of the field into different fundamental endeavors such as development of enzyme immobilization techniques, research …
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From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly
<p>The process of immobilizing enzymes onto solid supports for bioreactions has some compelling advantages compared to their solution-based counterpart including the facile separation of enzyme from products, elimination of enzyme autodigestion, and increased enzyme stability and activity. We …
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Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation
… for novel mitigation approaches. The use of enzymes such as laccase as biocatalyst has been recognised as a promising approach for micro-pollutants removal. However, rapid denaturation of the free enzyme and its difficulty in recycling and reuse restrict its wider application, and efficient …
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Fabrication of Novel In-Situ Remediation Tools for Unconventional Oil Contamination
… the final phase of study focused on finding enzyme mixture for biodegradation of PAHs contaminated sites for water and soil treatment. In this regard, screening of indigenous bacteria, identification of involved enzymes, and biodegradation tests were carried out. Several combinations of the …
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Nano- and micro- electrodes: applications in the biosensing field
… which were based on the glucose oxidase immobilization and in the anodic oxidation of the enzymatically produced hydrogen peroxide. Indeed, a comparative study of the results obtained with four enzyme immobilization procedures was carried out. The work highlighted that the high …
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Directing the self-assembly of short peptide derivatives
… exploiting different strategies to immobilize enzymes, thereby adding spatial control over the self-assembly process. We functionalised surfaces with bioinspired polydopamine and polyphenol coatings to study the effects of enzyme surface localisation and surface release on the self-assembly …
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Mikrobinių lipolizinių fermentų imobilizavimas ir imobilizuotų preparatų analizė /
… is currently being paid to the microbial enzyme class of lipases (E.C. 3.1.1.3), whose functional versatility and unique conformational structure allow the hydrolysis and synthesis of triacylglycerols. These properties are advantageous in a wide range of industrial applications, but the …
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Design, development and characterization of nanostructured electrochemical sensors
… detection, enzymatic detection and dual-enzyme cascade detection. The study is described over five main chapters with a sixth providing a summary of the material presented and perspectives for the future. Chapter 1 provides an introduction to the field of the electrochemical biosensors …
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Biological and biomimetic oxygen electro-reduction by enzymes
Enzyme catalysts are a viable alternative to the traditional platinum based catalysts for the complete oxidation of complex fuels. Efficient oxidation of multi-carbon compounds, selective catalytic activity eliminating the need for fuel purification, and high performance under mild temperature and …
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Nanostructured carbohydrate-derived carbonaceous materials
… structures in the fields of biotechnology (e.g. enzyme immobilization) and medicine (e.g. as drug micro-containers). Complimentary to these hard templating routes, a combined HTC / soft templating route for the direct synthesis of ordered porous carbonaceous materials was also developed. After …
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Cell-Free Biosystems Comprised of Synthetic Enzymatic Pathways: Development of Building Blocks, Immobilization of Enzymes, Stabilization of Cascade Enzymes, and Generation of Hydrogen
… However, it is essential to produce (purified) enzymes at low costs and stabilize them for long periods to decrease biocatalyst costs. Thermophilic recombinant enzymes as building blocks were discovered and developed: fructose 1,6-bisphosphatase (FBP) from Thermotoga maritime, phosphoglucose …
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DEVELOPMENT OF CRISPR-CAS IMMOBILIZATION SRATEGIES FOR THE FABRICATION OF A BIOSENSING PLATFORM
… the development of different strategies for the immobilization of the CRISPR-Cas enzyme, to create an integrated platform for the detection of DNA samples. In recent years, the CRISPR-Cas based diagnostic is emerging as a promising technology for the detection of infectious disease in point of …
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