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Showing 1 to 4 of 4 for “"enzyme characterization"”.
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Entwicklung einer modellbasierten Hochdurchsatztechnik zur Enzymcharakterisierung mit Schwerpunkt auf Langzeitstabilität
A new high throughput technique for enzyme characterization with specific attention to the long term stability, called "Enzyme Test Bench", is presented. The concept of the Enzyme Test Bench consists of short term enzyme tests in 96-well microtiter plates under partly extreme conditions to predict …
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Biochemical Characterization of the DUF3328 Protein in the Biosynthesis of Cyclic Peptide Cyclochlorotine
… an unusual feature among biosynthetic enzymes. To elucidate their biochemical roles, I purified CctR and CctP2 from Sf9 insect cells, overcoming challenges posed by their membrane association and intrinsic disorder. In vitro assays demonstrated that CctR is a copper-dependent enzyme …
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Pathway optimization and engineering for biofuel production
… in vivo will not only decrease the in vitro enzyme load in biomass pretreatment, it will also reduce the diauxic shift in pentose sugar utilization, thus significantly reducing the high economics of biofuel processes. More than engineering microbes to more efficiently utilize the biomass …
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Production and characterization of two AA3 enzymes from Arabidopsis thaliana
Enzymes involved in the synthesis and degradation of complex carbohydrates are classified in the carbohydrate-active enzyme database (CAZy). One of the classes, auxiliary activities (AA), consists of redox enzymes that assist other carbohydrate-active enzymes. Within this class, the AA3 family, …