Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 35 for “"enzyme catalysis"”.
-
Computational Approaches for Understanding and Redesigning Enzyme Catalysis
… specificity and catalytic efficiency of many enzymes make them attractive for applications ranging from therapeutics to chemical manufacturing. However, it remains challenging to identify the specific structural and dynamic mechanisms underlying the catalytic power of enzymes, which has …
-
Computational approaches to understand the atomistic drivers of enzyme catalysis
Enzymes readily perform chemical reactions several orders of magnitude faster than their uncatalyzed versions in ambient conditions with high specificity, making them attractive design targets for industrial purposes. Traditionally, enzyme reactivity has been contextualized through transition-state …
-
Snapshots of radical enzyme catalysis : the crystal structures of biotin synthase and lysine 5,6-aminomutase
… and adenosylcobalamin-dependent radical enzymes, the crystal structure of lysine 5,6-aminomutase was determined to 2.8 [angstroms] resolution. Like biotin synthase, the structure of lysine-5,6-aminomutase adheres to the general theme of barrels or barrel-like structures for enzymes that …
-
How life uses sound to reorganise matter: The role of anharmonic phonon exchange in enzyme catalysis
Biological enzyme catalysis is the name for life's ability to rapidly invoke coordinated rearrangements of molecular structures using highly specialised, relatively-large functional proteins called enzymes. It is well established that enzymes reduce the activation energy barrier, which vastly …
-
Development of an organo-and enzyme-catalysed cascade reaction
… (Scheme 1). The reaction combines organo- and enzyme-catalysis in an aldol/aldol cascade for the first time. The bicatalytic, three-component assembly involves two aldol reactions: an organocatalysed reaction and an aldol reaction catalysed by an aldolase. The feasibility of the one-pot …
-
In Search of Catalytic Proficiency: The Importance of Enzyme Conformational Change to Orotidine 5'-Monophosphate Decarboxylase Catalysis
… enzymology, the role of conformational change in enzyme catalysis has been recognized as an important one. This research has shed light on the conformational changes that take place when substrate binds OMPDC and how the two events are coordinated.
-
On the theory of Brownian aggregation in colloidal dispersions
… systems and affect cellular organization, enzyme catalysis, protein aggregation, and macromolecular binding. The primary focus of the thesis is on the fundamental study of this problem.
-
DIRECTED EVOLUTION FOR THE DESIGN OF NEW CATALYSTS
… of understanding the fundamental principles of enzyme catalysis and has gained recognition as a leading tool for understanding protein folding, structure, and function. De novo computational design coupled with directed evolution can yield catalysts with novel functions and improved catalytic …
-
Synthesis of Isotopically Labeled Fe and S-alkylated Iron-Sulfur Clusters
Radical S-adenosylmethionine (SAM) enzymes (RS enzymes) use a 3:1 site-differentiated [Fe₄S₄]⁺ cluster to reductively cleave the SAM cofactor and generate a 5’-deoxyadenosyl radical intermediate (5’-dAdo•) that regio- and stereospecifically abstracts an H-atom from the target substrate. It has been …
-
Molecular Details Of The Mitochondrial Iron Sulfur Cluster Assembly Pathway
… prosthetic group involved in electron transfer, enzyme catalysis, and regulation of gene expression. Their biosynthesis requires complex machinery located within the mitochondrion since free iron and sulfide are extremely toxic to the cell. Defects in this pathway results in several diseases such …
-
Studies on maize beta glucosidase gene-enzyme system
… the catabolism of various ß-D-glucosides. The enzyme expressed in maize sporophytes is encoded by a highly polymorphic locus, Glul (chromosome 10). In the present study, maize ß-glucosidase was purified to homogeneity by using differential solubility and chromatography. The enzyme is soluble …
-
Statistical Kinetics and Nonequilibrium Thermodynamics of Driven Systems: Stochastic Methods and Applications to Single-Molecule Biophysics
… influence of conformational interconversion on enzyme catalysis; and characterizing the nonequilibrium thermodynamics of generalized biomolecular machines. For enzymatic turnover reactions, it is found that the turnover rate reduces to the celebrated Michaelis–Menten functional form when …
-
Enzyme engineering by directed evolution and applications in cellular imaging and proteomic mapping
… the native cellular context. A wide panoply of enzyme-mediated labeling methodologies have been developed that couple the power of enzyme specificity with the functional diversity of small molecules to specifically tag proteins within the crowded heterogeneous intracellular environment for a …
-
The roles of redox active cofactors in catalysis : structural studies of iron sulfur cluster and flavin dependent enzymes
… and have evolved to take on many functions in enzyme catalysis. Two cofactors, flavin adenine dinucleotide (FAD) and [4Fe-4S] clusters, were originally determined to aid in electron transfer and redox chemistry. However, additional activities for these cofactors continue to be discovered. The …
-
Ubiquitin Conformational Dynamics and Hydration Shell Dynamics by Solid State NMR
… dynamics is essential for understanding the enzyme catalysis, protein molecular recognition, and the entropy changes during protein-ligand binding. Nuclear Magnetic Resonance (NMR) is an ideal biophysical experimental tool for the study of protein dynamics because it can be used to study …
-
Large scale quantum mechanical enzymology
… for the Bacillus subtilis chorismate mutase (CM) enzyme is made. Averaged energies of activation and reaction are presented for the rearrangement of chorismate to prephenate in CM and in water, for system sizes comprising up to 2000 atoms. Compared to the uncatalysed reaction, the calculated …
-
Elucidating the Biosynthesis of Nucleocidin, a Fluorinated Natural Product Produced by Streptomyces Calvus
… (C-F) bonds represents a very rare form of enzyme catalysis relative to other organohalogens. Instead of an oxidative mechanism, desolvation and nucleophilic incorporation of fluoride is required. Nucleocidin, produced by Streptomyces calvus, is a novel fluorinated analog of adenosine. The …
-
Ensemble methods in computational protein and ligand design : applications to the Fc[gamma] immunoglobulin, HIV-1 protease, and ketol-acid reductoisomerase systems
… binding as well as reaction rate enhancement in enzyme catalysis. First, we evaluate the solvent screened energetics of immunoglobulin G (IgG):Fc[gamma] receptor binding using molecular mechanics, Poisson-Boltzmann surface area (MMPBSA) models. We assess the role IgG1 linked glycans play in …
Page 1 of 2