Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"enzyme assay"”.
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Development of a phosphotriesterase enzyme assay for determination of organophosphate pesticides
… in the development of novel phosphotriesterase enzyme assays to measure paraoxon and other representatives of the organophosphate pesticides. The assay is based on a substrate-dependent change in pH at the local vicinity of the enzyme. This enzyme, which was labeled with fluorescein …
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Development of coupled enzyme assay and in vitro synthetic biology approach for glycosylation pathway characterization
… is the development of reliable kinetic assays and constructing biologically relevant substrate arrays. Presently used assays are disadvantaged by either being endpoint assays or continuous ones compromised by lack of specificity and sensitivity, or have high cost. Chemically synthesized …
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Synthesis and Biological Evaluation of Novel Glycomimetics
… needed to increase the overall yield. Also, an enzyme assay has to be performed to confirm the biological activity of the synthesised compound. The third chapter describes the synthesis of glucose-6-phosphoramidic acid and glucose-6-triazole derivatives as inhibitors of HK II and the biological …
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Investigation of an atypical protoporphyric family in South Africa
… from this family, established a ferrochelatase enzyme assay in our laboratory, and shown normal ferrochelatase enzyme activity in affected subjects.
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Microfluidic concentration-enhanced single cell enzyme activity assay
… platform for the direct measurement of enzyme activities from selected single cells without disrupting their extracellular context. We develop modules to: enhance enzyme assay sensitivity by microfluidic confinement, interface microfluidic devices with selected single cells, enable …
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Characterizing and Overcoming Resistance to Aminomethylspectinomycins in Gram-negative Bacteria
… a chromosomally encoded aminoglycoside modifying enzyme (AME). In this study, we follow up on this observation and examine the extent to which the amSPCs are substrates for AMEs through adenylation (ANTs) and phosphorylation (APH). APH(9)-Ia and ANT(3")(9) were expressed in E. coli BL21(DE3) and …
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Development of an organo-and enzyme-catalysed cascade reaction
… (Scheme 1). The reaction combines organo- and enzyme-catalysis in an aldol/aldol cascade for the first time. The bicatalytic, three-component assembly involves two aldol reactions: an organocatalysed reaction and an aldol reaction catalysed by an aldolase. The feasibility of the one-pot …
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Carbohydrate-Interacting Proteins from Two Nostoc (Cyanobacteria) Species
… obtain amino acid sequence information from the enzymes (glycosyltransferases) that are responsible for producing the extracellular polysaccharide (EPS) of Nostoc commune DRH1, ultimately to allow the transfer of this capacity to another organism. Two artificial substrates were synthesized for …
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Analysis of cathepsin H-deficient mice and identification of a dense cluster of placenta specific peptidase genes
… by southern-northern-western blot analysis and enzyme assay, the cathepsin H deficient mice were analyzed. The first macroscopic observation didn’t show any alteration. The cathepsin H deficient mice are fertile and grow normally. Also histological studies didn’t show also any pathological sign. …
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Water-Soluble Vitamin Requirements of Tilapia (Sarotherdon Mossambicus Peters and S. niloticus Linnaeus)
… analyses of proximate fish carcass composition, enzyme assay (GOT, GPT for pyridoxine test), and mineral contents of fish were used in correlating their vitamin requirements. Poor growth, high and variable FCR and lowered SGR were obtained in CAP, BIO, Rib, B12 deficient fish. Dietary deficiency …
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Secretion of active recombinant phytase from stably transformed soybean cells
… was constructed and bombardment was optimized as assayed by β-glucuronidase reporter gene expression. A somatic embryogenesis approach was used for soybean regeneration from culture. The efficiencies of embryo induction and embryo conversion to form roots and shoots were compared in commercial …
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Design, Synthesis and Biological Evaluation of Inhibitors of Polysialyltransferases PST and STX. Design, synthesis and biological evaluation of a range of N-modified mannosamines, sialic acids and analogues from in silico screening as inhibitors of PolySia-NCAM biosynthesis with anti-migration activity.
… of the polysialyltransferases (polySTs), the enzymes responsible for polysialylation of neural cell adhesion molecule (NCAM), has been shown to abolish cell migration. PolySia-NCAM is thus a highly attractive novel therapeutic target. A library of potential polyST inhibitors has been …
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A new mass spectrometric assay of N8-acetylspermidine deacetylase and partial purification of the enzyme
<p>A new enzyme activity assay has been developed for the target N<sup>8</sup>-acetylspermidine deacetylase, a not-well-studied but essential enzyme in the polyamine interconversion and reutilization pathway.</p><p>The enzyme assay, based on mass spectrometric detection of a specific reaction …
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Glucose and Glucosamine Derivatives as Novel Low Molecular Weight Gelators
… preliminary study on using one of the gelator in enzyme assay has shown that it is possible to utilize the hydrogels to immobilize enzymes. However, future research can explore further on the applications of these gelators.
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The Development of a Time-Resolved, Vesicle-Based, Fluorescence Assay for the Activity of the Lipid Kinase Pik1
… from the trans-Golgi (1, 2). Pik1 is a soluble enzyme, and the small myristoylated, Ca2+ binding, EF hand protein, Frequenin (Frq1) facilitates its membrane localization (3). It has been suggested that Pik1 finds its substrate, phosphatidylinositol (PI), within phospholipid bilayers with …
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Quantification of total microbial biomass and metabolic activity in subsurface sediments
… cells, activity measurements with radiotracer assays and the analysis of porewater constituents are currently being used to explore the subsurface biosphere, alongside with molecular biological analyses. However, many of these techniques reach their detection limits due to low microbial …
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Soil phosphatase activity: sources of variability and relationships with phosphorus fractions
… inorganic P (Pi) and catalyzed by phosphatase enzymes produced by soil organisms. This M.S. thesis addresses phosphomonoesterase (PME), a phosphatase that catalyzes the terminal step of Po mineralization. The rate of catalysis by soil PME, often referred to as activity, has been measured …
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Role of Fatty Acid omega-Hydroxylase 1 and Abscisic Acid in Potato Tuber Suberin Formation
… activity was confirmed through an in vitro enzyme assay with recombinant protein. Following an in silico analysis of the CYP86A33 promoter region, which identified many ABA-responsive promoter elements, an extensive analysis of the effects of ABA on gene expression and suberin biosynthetic …
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ELUCIDATION OF MECHANISMS GENERATING 5-HYDROXYMETHYLCYTOSINE (5hmC) IN MAMMALIAN MITOCHONDRIA
… in the pathway of DNA demethylation. Tet enzymes catalyze this hydroxylation in a 2-oxoglutarate and Fe2+ dependent manner. We have recently reported significant levels of 5mC and 5hmC modification in immunoprecipitates of mammalian mitochondrial DNA(mtDNA). We provide the first evidence …
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Evaluation of anti-gout potential of selected Malaysian medicinal plants
… plants for anti-gout activity using in vitro enzyme assay; to optimize the extraction conditions of the three selected plants using response surface methodology (RSM); to determine the cell viability of the three extracts towards RAW 264.7 murine macrophage cell line; to investigate the in …
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