Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"enzymatic properties"”.
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Studies on some enzymatic properties of mitochondrial propionyl carboxylase
… The apparent equilibrium constant for the enzymatic propionyl-CoA carboxylation reaction at pH 8.15 and 37°c is 8.1 x 10<sup>-3</sup> and the Δ F°<sub>310</sub> calculated from this constant is 2970 calories per mole.
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Copper/zinc Sods of Salmonella Typhimurium: Enzymatic Properties and Correlation to Pathogenesis
"We have however, noted three significant differences between SodCI and SodCII: dimerization, tethering, and protease resistance. SodCI is a dimer and is not released from the periplasm by osmotic shock. Thus SodCI is ""tethered"" in the periplasm by some non-covalent interaction. In contrast, …
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An investigation of the physiological roles and enzymatic properties of invertases in tobacco and hybrid poplar
Plant invertases (EC 3.2.1.26) represent a multi-gene family of β-fructofuranosidases that perform integral roles in several biochemical processes. The central importance of this family of enzymes to plant growth and development has made them a primary target of investigation in plant biology. …
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Studies on the mechanism of action of propionyl-CoA carboxylase
… to a state of near nomogeniety, and some of its enzymatic properties relating to substrate binding and mechanism of action have been studied. The enzyme was not found to catalyze the incorporation of solvent tritium at the c-carbon of propionylâ CoA in the absence of ATP. Absolute …
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Development of universal stoichiometric coefficients for modeling microalgal cultivation systems
… process modeling derived from the conserved enzymatic properties for seven algae species using 11 genome-scale models. The model parameters include yield coefficients for algae grown under various energy inputs (photoautotrophic and heterotrophic), nitrogen sources (ammonia and nitrate), and …
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Foamy Virus Enzymes - Activity, Regulation and Resistance
… were purified and compared. The biophysical and enzymatic properties of the two enzymes were similar. However, their behavior towards the nucleoside inhibitor azidothymidine is different. This nucleoside analog inhibits the replication of foamy viruses by terminating polymerization. Prototype …
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Cellular uptake and biological activity of synthetic hammerhead ribozymes
… are a class of ribonucleic acid that possess enzymatic properties. They can inhibit gene-expression in a highly sequence specific manner by catalysing the trans-cleavage of target RNA. The potential use of synthetic hammerhead ribozymes as novel anti-brain tumour agents was investigated in …
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Determination of Pancreatic and Salivary Amylase By Enzyme Immunoassay and Their Prevalence in Hyperamylasemic Patients
… the organ source and are based entirely on the enzymatic properties of amylase to produce a measurable product or byproduct. The determination of pancreatic amylase is important in the diagnosis of acute pancreatitis. Most commercially available tests for amylase employ the measurement of the …
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Plant cell wall degrading enzymes and their potential application to biofuel production
… to biofuels. Microbes have evolved a plethora of enzymatic strategies for hydrolyzing plant cell wall into its constituent sugars for subsequent fermentation to biofuels. Therefore, microorganisms are considered an important source of biocatalysts in the emerging biofuel industry. …
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Advancing Technologies for Precision Medicine: From Liquid Biopsy to Single-Molecule DNA Sequencing
… cleave nucleotides for detection. Therefore, enzymatic activity of lambda exonuclease (λ-exo) was characterized in both solution and solid phase to determine the reaction conditions compatible with nanopore detection. Our results indicate that λ-exo digestion efficiency decreases at high salt …
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Purification and characterization of a smooth muscle myosin phosphatase from turkey gizzards
… sequencing. However, as isolated, SMP-III is enzymatically distinct from either type 1 or type 2 phosphatases. Limited tryptic digestion of SMP-III modulates the phosphatase activity such that it behaves like a typical type 1 phosphatase. The change in enzymatic properties correlates with the …
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Coordination Geometries in Metallobundles Enforcing Oxidative and Hydrolytic Catalysis and Designing Biomaterials for Use As Antimicrobials
… features. Defining the structural and functional properties of hydrolases in DF proteins would facilitate creation of catalysts for bioremediation purposes. We further apply minimalist design approaches to redesign natural proteins displaying no inherent catalytic properties (or showing marginal …
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DYNAMICS OF NUCLEOSOME REMODELING BY ATP-DEPENDENT CHROMATIN REMODELERS
… aberrant SWI/SNF complexes affect fundamental enzymatic properties such as ATPase and processive nucleosome remodeling. ISW2 remodelers sense and respond to the length of linker DNA separating the nucleosome and centers nucleosome. Histone octamers are perceived as a mostly static structure …
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Discovery Of Inactive E* Conformations In Thrombin And Other Vitamin K- Dependent Clotting Proteases
… studies revealed that Na+ enhances the enzymatic properties of these proteases from a low activity, E, to a high activity: E:Na+) conformation. However, investigation of the effects of Na+ on these proteases has mainly focused on the thermodynamics of interaction and the resulting …