Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"enzymatic pathway"”.
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Synthetic enzymatic pathway conversion of cellulosic biomass to hydrogen
… pot to catalyze complex conversions: synthetic pathway biotransformations (SyPaB). An exemplary technology in this burgeoning field is the production of hydrogen from biomass sugars. Lignocellulosic biomass, which includes agricultural residues, energy crops, and industrial waste streams, is an …
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Enzymatic Production of Cellulosic Hydrogen by Cell-free Synthetic Pathway Biotransformation(SyPaB)
… from cellulosic materials and water by synthetic pathway biotranformation (SyPaB), and 2) to increase the hydrogen production rate to a level comparable to microbe-based methods (~ 5 mmol H2/L/h). Cell-free SyPaB is a new biocatalysis technology that integrates a number of enzymatic reactions from …
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Synthesis of proteasome inhibitors: analogues of nelfinavir
… 1 to be involved in the inhibition of Akt pathway – an enzymatic pathway that results in arrest of apoptosis and prolongation of cell survival, which is also a very common mechanism in many types of cancers. These characteristics made nelfinavir 1 a very promising target for the anticancer …
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In Vitro Synthetic Biology Platform and Protein Engineering for Biorefinery
… formation. In this work, the in vitro synthetic (enzymatic) biosystem is used to produce a future carbon-neutral transportation fuel, hydrogen, and two high-value chemicals, a sugar phosphate and a highly marketable sweetener, representing a new portfolio for new biorefineries. Hydrogen gas is a …
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High-Yield Cellulosic Hydrogen Production by Cell-Free Synthetic Cascade Enzymes: Minimal Bacterial Cellulase Cocktail and Thermostable Polyphosphate Glucokinase
… national energy security. Cell-free synthetic pathway biotransformation (SyPaB) is the implementation of complicated chemical reaction by the in vitro assembly of numerous enzymes and coenzymes. Two of the biggest challenges for its commercialization are: effective release of fermentable sugars …
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Enzymatic fuel cells via synthetic pathway biotransformation
… three largest technical challenges for emerging enzymatic fuel cell technologies are incomplete oxidation of most fuels, limited power output, and short lifetime of the cell. Synthetic pathway biotransformation is a technology of assembling a number of enzymes coenzymes for producing low-value …
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Exploring Prokaryotic Thiamin Biosynthesis: Mechanistic Studies On Thiamin Thiazole Synthase And Pyrimidine Synthase
… The in vitro reconstitution of this enzymatic pathway has been achieved and detailed insights have been obtained, however, the very small quantity of product produced in this in vitro reconstitution prevented direct characterization of its structure. We were able to study the last few …
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Metabolomic Approaches in Parasitology
Metabolic pathways and networks are crucial for maintaining cellular functions and sustaining life. Understanding these pathways in parasites may reveal novel targets for therapeutic application if they are sufficiently different from mammalian systems. My work investigated two such pathways: …
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Cell-Free Biosystems Comprised of Synthetic Enzymatic Pathways: Development of Building Blocks, Immobilization of Enzymes, Stabilization of Cascade Enzymes, and Generation of Hydrogen
… (in vitro) biosystems comprised of synthetic enzymatic pathways would be a promising biomanufacturing platform due to several advantages, such as high product yield, fast reaction rate, easy control and access, and so on. However, it is essential to produce (purified) enzymes at low costs and …
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Dna Polymerase Θ (Polq) and The Cellular Defense Against Dna Damage
… sensitivity in mammalian cells, the relevant enzymatic pathway has not been identified. Here we define the specific mechanism by which POLQ restricts harmful DNA instability. Our experiments show that <em>Polq</em>-null murine cells are selectively hypersensitive to DNA strand-breaking agents, …
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Capillary electrophoresis instrumentation and applications for the analysis of neural systems
… by our collaborators indicating a lack an enzymatic pathway. While serotonin is an important and well-studied tryptophan metabolite, we have begun expanding our system to detect metabolites in the kynurenine pathway. In fact, this pathway is responsible for the majority of tryptophan …