Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"enzymatic assay"”.
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Biopterin metabolism in health and disease
… was measured using a Crithidia fasiculata assay. Secondly the urine concentration of neopterin and biopterin was estimated by high performance liquid chromatography. Thirdly the urine concentration of tetrahydrobiopterin was determined by an enzymatic assay using phenylalanine hydroxylase. …
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Separation and Structure Characterization of Biologically Important Substances
… against the L1210 murine leukemia cell line. The enzymatic assay indicated, however, that Cu$\sp{2+}$ (CuSO$\sb4$), which is one of the additives in the commercial Yucca schidigera extract, showed potent urease inhibition activity.
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Bioaffinity-based Concept for Glyphosate Detection
… and substrate concentrations using the Ellman enzymatic assay. AChE activity is best maintained at 50 mU with 3 mM DTNB and 5 mM ACth, and storage at 4°C assures stability. The study also exposes glyphosate's inhibitory effect on AChE, demonstrating its potential hazards to human health and the …
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Identification and Quantification of Protein Carbonylation by Mass Spectrometry
… spectrometry (LC/MS), Western Blots, and enzymatic assay.
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Towards Rapid Measurement of Non-Nucleoside Reverse Transcriptase Inhibitors in a Portable, Low-Cost Platform
… settings. In response, we developed a rapid enzymatic assay for the measurement of non-nucleoside reverse transcriptase inhibitors (NNRTIs) based on inhibition of DNA synthesis using an inexpensive, portable instrument. This assay is entitled the REverse transcriptase ACTivity (REACT) assay. …
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Identification and Characterization of A Zebrafish Glutathione S-Transferase Pi
… subsequently cloned, expressed, and purified. An enzymatic assay for purified GST Pi was established using 1-chloro-2, 4-dinitrobenzene (CDNB) as a substrate. A pH-dependency study was performed, and the results indicated that the zebrafish GST Pi was active over a pH range of 6 and 8 with optimal …
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Assessment of potential antibacterial drug targets
… from various bacteria. Additionally, different enzymatic and binding assays were applied to assess kinetic parameters of KynB, Ddl and FolC, and compound library screenings were carried out for Ddl. In parallel, the genetic validation of these targets was being carried out by the Dstl.KynB is an …
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D-glucaric acid excretion: its positive association with gender, tobacco, caffeine, marijuana, and vegetarianism in humans
… of menstruation. DGA was determined by an enzymatic assay and recorded as micromoles D-glucaro-1,4-lactone/gm creatinine. There was a positive association between total DGA excreted and the use of marijuana, caffeine, and tobacco products, the heaviest users excreting significantly higher …
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Growth and metabolism of Aquaspirillum gracile
… this hypothesis was confirmed by a more specific enzymatic assay for gluconate. Preliminary evidence is presented for the accumulation of galactonic and L-arabonic acids in galactose and L-arabinose cultures, respectively.
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Lysine Polyphosphate Modification Regulates Enzymatic and Signaling Functions in Eukaryotic and Bacterial Systems
… transferase 2 (NMT2). Using a fluorescence-based enzymatic assay, we observed that KPM of NMT2 reduces the release of myristoyl groups, consistent with partial inhibition of its catalytic activity. These results provide functional evidence that polyP association with lysine-rich regions can …
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Sex- and Training-Related Differences in Metabolic, Sex Steroid and Growth Hormone Responses During Prolonged Exercise
… forearm. GLU and HLa levels were measured with enzymatic assay kits; hGH, E(,2), and T levels were measured by radioimmunoassay. The main purposes of the study were to determine: (1) the relationship between sex steroid (E(,2), T) profiles and hGH responses, (2) the influence of endurance …
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Enzyme sources for urinary oxalate measurements
… Aldrich Ltd. Oxox were researched. An optimum enzymatic assay, substrate specificity, optimum buffer and optimum pH for Sigma Oxox were investigated prior to its purification. The purification of the enzyme led to the finding that Sigma Oxox was unstable in a number of salt buffers, and …
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A computational study of DNA four-way junctions and their significance to DNA nanotechnology
… imaging, computational modelling, and a novel enzymatic assay is exploited to characterize the three-dimensional structure and catalytic function of a DNA tweezer-actuated nanoreactor. The analyses presented here show that rational redesign of the four-way junctions in the device enables the …
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Investigating neural responses in models of neurocysticercosis
… receptors nor acid-sensing ion channels. An enzymatic assay showed that the larval homogenate contains high levels of glutamate, explaining its acute excitatory effects on neurons. In the second part of my thesis I demonstrate that longer-term incubation of Taenia crassiceps homogenate with …
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Genes involved in thiol release from cysteine and glutathione conjugates by Saccharomyces cerevisiae during wine fermentation
… that Irc7F cleaves L-cysteine in vitro (enzymatic assay with purified Irc7Fp) and in vivo (growth in L-cysteine in fermentative-like conditions), suggesting that it plays a biological role in yeast sulfur metabolism. Moreover, yeast selected for growth on L-cysteine showed improved 4MMP …
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Design and synthesis of novel non peptidomimtic beta-secretase inhibitors in the treatment of Alzheimer's disease
… a FRET (fluorescence resonance energy transfer) enzymatic assay (test A). With the aim to enhancing the biological activity of the lead compound, we applied the molecular simplification approach to design and synthesize novel heterocyclic compounds related to lipocrine, in which the …
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Investigating the regulation of APOBEC mutagenesis in cancer
… downregulated when p53 activity is promoted. An enzymatic assay for cancer-associated APOBEC activity is established. Reverse transcriptase inhibitors, which inhibit LINE-1 activity, appear to modulate APOBEC3B expression and associated enzymatic activity. This appears to occur when cells are …
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The effect of anabolic-androgenic hormones on postprandial triglyceridaemia and lipoprotein profiles in man
… (GGE), lipoprotein concentrations by immuno and enzymatic assay, and postprandial triglyceridaemia by a standardised oral fat tolerance test (65g/m² ). HDLC decreased in male bodybuilders (0.94±0.30 vs 0.70±0.27 mmol/L, p=0.004; x ± SD) and female bodybuilders (1.3±0.5 vs 0.8±0.2 mmol/L) ON …
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Design, Chemical Synthesis and Biological Evaluation of Potential New Antiviral Agents
… pure N- and C-acylated compounds. A preliminary enzymatic assay indicated a good activity in the early prepared compounds of the series, promising for the following in vitro tests on HIV infected cells of each molecule in the whole series. In addition, these compounds were tested against other …
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Novel Inhibitors for Isocitrate Lyase as a Potent Antitubercular Agent for Mycobacterium Tuberculosis
… to set up crystallisation trials and in vitro enzymatic assay was performed on ICL1 inhibitors. The synthesis of D/L-threo-2-methylisocitrate and the methylation of five other drug candidates were described. Both co-crystallisation and soaking techniques were performed to obtain an …
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