Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"envelope proteins"”.
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The study of highly pathogenic emerging zoonotic virus envelope proteins through pseudotyped virus generation
… viruses (PVs), incorporating the viral envelope protein of interest which constitutes the primary surface antigen. This permits the serological detection of neutralising antibodies without the need to handle live virus, as well as other viral entry studies. Hence, PVs are increasingly …
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From Fossils to Function: Exploring the Evolutionary And Functional Diversity Of HERV Envelope Proteins Across Primate Lineages
… the tropism of ancestral and current HERV Env proteins. The first part of the project focused on the HERVK(HML2) group, which is significant due to its recent integration into the human genome and its functional conservation. While ERVs are found in all vertebrates, HERV-K is specific to …
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Human papillomavirus 11 induces abnormal expression of two cornified cell envelope proteins: loricrin and the small proline rich protein 3
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Probing surface protein patterning in biological systems using fluorescence nanoscopy
… nanoscopy to study the patterning of proteins in biological surfaces at a high spatial resolution of 25 nm. We also developed new analytical methods to extract quantitative information on protein arrangement within biological substrates to elucidate key phenomenon such as signal …
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Translational Frontiers for Chikungunya Virus: New World Epidemic, New Strains, New Therapeutic Targets
… antiviral drug targets was explored. As the envelope proteins of alphaviruses are replete with structure-maintaining disulfide bonds, inhibitors to the host enzymes responsible for disulfide bond formation—protein disulfide isomerase (PDI) family chaperones—were utilized as a tool to …
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Engineering Viruses for Gene Therapy: Isolating and Characterizing Murine Leukemia Virus With Improved Stability
… increased stability with various wild type envelope proteins, including amphotropic, ecotropic, 10A1, and VSV-G. Lastly, saturation mutagenesis at the site of the beneficial mutation identified MLV mutants with infectivity half-lives of ∼24 h at 37°C, nearly a 4-fold increase in …
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Antigenic profiling of the porcine reproductive and respiratory syndrome virus
… the antibody response against the entire set of envelope proteins (GP2, E, GP3, GP4, GP5 and M) upon infection with LV was investigated. Twenty-one antigenic regions were identified that are capable of inducing antibodies in pigs. Peptide-purified antibodies against four antigenic regions turned …
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GLYCOPEPTIDE ANALYSIS OF HIV-1 ENVELOPE PROTEIN USING HPLC/ESI-FTICR MS AND MALDI-TOF MS WITH ASIALOFETUIN ENRICHMENT AND SEPARATION TECHNIQUES
Glycosylation on proteins serve many biological roles and their heterogeneity can affect various biological functions. The site occupancy of the glycans as well as their various glycoforms makes glycans unique to analyze due to the fact that the variation in structure is not based on a genomic …
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Purification of the major envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions
… heterogeneity. Evidence indicates that the major envelope protein, GP5, is the primary target for a subunit vaccine. In native virions GP5 primarily exists as a disulfide linked complex with the membrane protein, M, which also possesses immunogenic properties. Recent studies report that the GP5/M …
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PCP and conformational motifs: applications in predicting mechanical strength, allergenicity of proteins and vaccine design
The functions and properties of proteins are mainly determined by their amino acid sequences and 3-dimensional structures. Motifs composed of amino acids that are either continuous in sequence or cluster in space play important roles in structures and functions of proteins. The functional …
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Studies on the stress response in Fusobacterium nucleatum.
… differential expression of cytoplasmic- and cell envelope-proteins induced by changes in pH were identified by two-dimensional gel electrophoresis. The results represent the first proteomic investigation of F. nucleatum. Twenty-two cytoplasmic proteins were found to have altered expression in …
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Characterisation of llama antibody fragments able to act as HIV-1 entry inhibitors
… entry into cells is mediated by the functional envelope spike, which consists of trimers of gp120 bound to gp41. Most variants of HIV-1 enter cells through attachment of the envelope spike to the main cellular receptor CD4, allowing interaction with a co-receptor and eventually fusion of viral …
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Influenza Viral Protein Interaction Detecting Methods Development and A.M1-host Interplay Network Investigation
… protein, known as a linkage, connects external envelope proteins and internal core proteins, and also plays significant role in virus structure morphology. However, the other functions of M1 has not been defined. In this study, we used a bait-fishing strategy by applying over-expressed …
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Binding sites in the nuclear envelope for cytoplasmic glucocorticoid receptor complex
… peak on a 5 - 20% sucrose gradient. Nuclear envelopes isolated from purified rat liver nuclei were found to contain binding sites for the partially purified cytoplasmic triamcinolone acetonide-receptor complex. The binding constants showed two saturable high affinity binding sites and the …
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Natural Killer Cell Responses in Chronic Hepatitis C Virus Infection
… 18 amino acid peptides (OLPs) comprising the HCV envelope (E1, E2) and nonstructural (NS3) sequences. Specificity controls consisted of a pool of overlapping hepatitis D virus peptides and samples without peptide addition. As a readout for NK and T cell function, the frequency of IFNγ+ NK cells …
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Extent of genotype-specific humoral immunity produced on infection with hepatitis C virus: relevance for serological screening and diagnosis
… amino acid sequence divergence in their encoded proteins. Sequence variability is not uniform across the genome, being greatest in the regions encoding envelope proteins and most restricted in the core gene. Sequence variation in the antigenic determinants in core, NS3, NS4A/B and NS5A/B …
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Studies of viral and cellular proteins involved in herpes simplex virus type-1 egress
… by co-ordinated activity of several virus glycoproteins. The virions are first assembled and enveloped at trans-Golgi-network (TGN) or endosome membranes and then travel through a guided pathway that is directed towards the cell adherent points for secretion. Once secreted the vast majority of …
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A Nuclear Mechanoresponse to Morphogenesis Drives Acquisition of Neuroectoderm Lineage Competence During Pluripotent Cell Differentiation
… is a mechanosensitive, LINC-interacting, nuclear envelope protein that can regulate histone-modifying enzyme activity. Emerin knock-down also resulted in impaired neuroectoderm differentiation. Interestingly, perturbations of contractility or LINC only led to an impaired differentiation phenotype …
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