Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"enterokinase"”.
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Recombinant Expression In Pichia pastoris System Of The Potassium Channel Inhibitor Peptide, Ts6.
… where the N-terminal was his-tagged and an enterokinase cleavage site was inserted. The X-33 Pichia clones were cultured in increasing concentration of ZeocinTM. After methanol induction, protein expression yielded around 4.7 mg/l Ts6. The purification of the his-tag Ts6 was done in two …
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Cloning and Overexpression of Yeast Cystathionine γ-Lyase
… it with an XhoI and BlpI restriction site and enterokinase sequence (creating a modified <i>CYS3</i>, <i>mCYS3</i>, gene), and ultimately spliced it into a pET-15b plasmid to create a recombinant plasmid. Then transformed the recombinant plasmid into BL21 E. coli cells. This enabled the …
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Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor
… The recombinant systemin was released by enterokinase and isolated by HPLC. After further purification, the physical characteristics including amino acid composition, peptide sequence and molecular weight of r-systemin were determined. When the recombinant peptide was applied to young …
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Molecular diversity and coat protein expression of Sweet potato leaf curl virus
… ìg/liter of bacterial culture. Digestion with enterokinase cleaved the fusion protein into a 42.5 kDa maltose-binding protein and a 29.4 kDa protein. The latter protein was identified by mass spectrometry analysis as the CP of SPLCV.
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Engineering functional recombinant proteins based on antibody domains and fragments of C. botulinum neurotoxin
… site, which is cleaved by a protease such as EK (Enterokinase) or FXa (Factor Xa) at specific residues within the sequence. The contrast in molecule activation will distinguish the best candidates. It was decided that targeting the tyrosine kinase receptor family was most suited to this research …