Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"enterochromaffin"”.
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Sensory Transduction in Enteroendocrine Cells
… potential (TRP) superfamily in I cells and enterochromaffin cells using the model tumour cell lines STC-1 and KRJ-I respectively.<br/>Electrophysiology and PCR was performed on STC-1and KRJ-I model cell lines to elucidate the channels that may regulate hormone release. In KRJ-I cells two …
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Stress-induced Alterations in Mast Cell Numbers and Proteinase-activated Receptor-2 Expression of the Colon:Role of Corticotrophin-releasing factor
… whether acute stress can increase mast cell and enterochromaffin (EC) cell numbers, and proteinase-activated receptor-2 (PAR2) expression in the rat colon. In addition, we aimed to investigate the involvement of corticotrophin-releasing factor in these stress-related alterations. Eighteen adult …
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Characterizing the Immunogenicity of Islet-like Cells derived from HLA-edited Human Pluripotent Stem Cells
… cells enriched for islet-like (ISL1+) or enterochromaffin-like (ISL1-SLC18A1+) cells. Finally, I leverage these new protocols to study how HLA proteins affect the immunogenicity of human pluripotent stem cell-derived islets. Using human pluripotent stem cells edited to lack cell-surface …
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Investigating the Enteroendocrine - Brain Axis: Ghrelin Cell and ECL Cell Physiology and Ghrelin Action on Mood and Complex Eating
… endocrine cells, such as the histamine producing enterochromaffin-like cell. My doctoral research encompasses multiple aspects of the ghrelin system, from physiological assessments of the ghrelin cell to evaluations of ghrelin action on cue-potentiated feeding and stress-induced depressive-like …
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Exploring INSL5 and RXFP4 in the gut-brain axis and their potential role in feeding behaviour
… techniques, we identified Rxfp4 expression in enterochromaffin, L and tuft cells in the colon, in neurons of the dorsal root ganglia (DRG) and nodose ganglia (NG), and in multiple brain regions associated with feeding. By combining Rxfp4-Cre mice with a cyclic adenosine monophosphate (cAMP) …
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Exploring the function and sensing mechanisms of human enteroendocrine cells using intestinal organoids
… insulinotropic polypeptide (GIP), and enterochromaffin (EC) cells, which secrete serotonin, or 5-hydroxytryptamine. GIP, largely known as one of the two gut-derived insulinotropic incretin hormones, alongside with glucagon-like peptide 1 (GLP-1), has recently regained popularity in the …
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Intrinsic Neural Control of Propulsion, Storage and Slow Transit in the Large Intestine.
… local mucosal reflexes, which release 5-HT from enterochromaffin cells, and stretch reflexes that determine the site of origin and propagation of the CMMC, facilitating propulsion.Collectively, these studies demonstrate that in addition to AH sensory neurons; specific interneurons within the …
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Characterisation of L-cell secretory mechanisms and colonic enteroendocrine cell subpopulations
… (Nts). Another major EEC subpopulation, enterochromaffin (EC) cells could be split into three groups, mechanosensitive and pro- inflammatory EC cells distinguished by Piezo2 and Tac1 expression, respectively and a third Sct-expressing group. Immunofluorescent labelling and RT-qPCR …
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Characterisation of human enteroendocrine cells in organoid models
… To study 5-HT secretion from intestinal enterochromaffin cells (ECs), organoids with labelled tryptophan hydroxylase 1 expressing cells (TPH1-Venus) were established from human duodenum, ileum and rectum. Bulk RNA sequencing was performed on duodenal ECs, and considered in parallel with …
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miR-10b Rescues Diabetes and GI Dysmotility Associated with a Leaky Gut
… such as interstitial cells of Cajal (ICCs), enterochromaffin (EC cells), enteric neurons, smooth muscle cells, gut immune cells, etc. that are normally dysregulated in these conditions. Our previous studies demonstrated that depletion of miR-10b in KIT+ cells has been shown to cause the …