Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"enrichment culture"”.
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Cobalamin-Enhanced Anaerobic Biotransformation of Carbon Tetrachloride
… at high CT concentrations by supplementing cultures with cobalamins. Three different cultures; an acetogenic pure culture-Acetobacterium woodii (ATCC-29683), a dichloromethane degrading enrichment culture, and a methanol enrichment culture were studied for this purpose.
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New approaches to culture and detection of verocytotoxin producing escherichia coli (VTEC) in clinical laboratory diagnosis
… investigation of the growth of non-0157 VTEC in enrichment culture using indirect conductimetry. The media developed for enrichment culture of 0157 VTEC was found to be inappropriate for enrichment culture of some non-0157 VTEC strains. This was particularly evident among members of serogroups …
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Surface Directed Monoclonal Antibodies against STEC Aid in the Reduction of Pathogen Detection Times from Food and Water
… non-O157 serogroups of STEC. Following a short enrichment step (4-6 hrs), two approaches were considered: (1) the filtration of enrichment culture through a gradient of filtration membranes (decreasing pore sizes), followed by capture using specific monoclonal antibody (mAb)-coated Dynabeads, …
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Microbial Growth On Anthropogenic Compounds By Reductive Dehalogenation Or Oxidation
… concentration. From the sediment microcosms, an enrichment culture was developed, with one dominant organism related to Desulfovirga adipica that was able to oxidize ethene coupled to sulfate reduction. Growth and metabolism in this enrichment culture was observed by quantative PCR and the …
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An Integrated Approach to Improving Efficiency in Photosynthetic Microbial Systems
… soda lakes, was previously used as inoculum for enrichment of a mono-cyanobacterial microbial consortium. In this thesis, the microbial composition and function of the productive haloalkaline lakes of origin were analyzed using metagenomics and metaproteomics (Chapter 2). This analysis showed …
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Redutive Dehalogenation of Chlorinated Alkanes by Novel Bacteria at the PetroProcessor of Louisiana Inc. Superfund Site
A reductively dehalogenating enrichment culture was established using chloroalkane-contaminated groundwater from the PetroProcessors of Louisiana, Inc. (PPI) Superfund site. Two novel, strictly anaerobic bacterial strains, designated as BL-DC-8 and BL-DC-9, were isolated from the enrichment …
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Discovery and Characterization of an Antibiotic from the Soil Bacterium <em>Bacillus</em> sp.
… Fifty four soil bacteria were isolated using enrichment culture techniques (including 37 <em>Rhodococcus</em>) and screened for antibiotic producers. BTHX2, a species of <em>Bacillus</em> was found to have activity against <em>Micrococcus luteus</em> and <em>Rhodococcus erythropolis</em>. …
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Micropollutants biotransformation by two nitrifying communities enriched from biofilm of a nitrification trickling tower
… Both inhibition studies and pure culture studies have been performed to test this hypothesis. By inhibiting ammonia oxidation in nitrifying activated sludge (NAS) with inhibitors including allythiourea and octyne, the biotransformation of a number of micropollutants was inhibited …
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Anaerobic transformations of Kepone by denitrifying bacteria
… bacteria grown on benzoate-nitrate medium. In enrichment cultures, Kepone was transformed more extensively than by pure cultures isolated from the enrichments. A gram negative-faculative rod, called K bacterium, transformed 8.8% of the Kepone in a benzoate-nitrate medium in the presence of 2000 …
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Surveying and harnessing the genetic, (meta)genomic, and metabolic potential of the deep carbonated biosphere
… isolated strain Bacillus megaterium SR7 through enrichment culture and serial passaging of McElmo Dome scCO₂ reservoir fluids. I then initiated process improvements including media and culturing optimization under 1 atm CO₂ that increased SR7 growth frequency under scCO₂ . After developing a …
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Isolation and Ecology of Bacterial Populations Involved in Reductive Dechlorination of Chlorinated Solvents
… (pristine) environments. Careful examination of enrichment culture dechlorination kinetics, 16S rRNA gene based analyses, and reductive dehalogenase gene targeted PCR approaches revealed that complete reductive dechlorination is carried out by multiple dechlorinators. Two new dechlorinating …
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Investigation of bacteria associated with Australian wine grapes using cultural and molecular methods
… throughout cultivation, as determined by plate culture, enrichment culture and PCR-DGGE. B. thuringiensis carried over into wine processing but did not grow in juice or wine and did not adversely affect the growth of Saccharomyces cerevisiae or Oenococcus oeni in liquid culture. B. thuringiensis …
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A Study of Antibiotic Resistance Plasmids in Fresh Produce and In Wastewater Environments Using Functional Approaches, Comparative Genomics and Abundance Analysis
… and also incF family incompatibility genes using culture-dependent and culture-independent approaches. During this research, I have isolated and characterized two ciprofloxacin resistanceencoding, multidrug resistance plasmids (pLGP4 and pHTP1) from fresh spinach. I also iii isolated six plasmids …
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Isolation, growth and epidemiology of campylobacter jejuni / coli
… selective blood agar (SBA) and a selective blood enrichment broth (SBEB) containing polymyxin (5,000 iu/l), trimethoprim (10 mg/i), rifampicin (10 mg/l) and cyclohexamide (100 mg/l) have been developed. Results from a comparison with four other media showed that the SBA gave the most isolations …
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Functional characterisation of alkane-degrading monooxygenases in Rhodococcus jostii strain 8
… R. jostii strain 8 was isolated from a propane enrichment culture using petroleum-contaminated soil as an inoculum. R. jostii strain 8 could grow on ethane, propane, butane, octane, naphthalene and some potential intermediates in alkane metabolism. Oxidation studies showed that R. jostii strain …
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Diversity of methane and short chain hydrocarbon degrading bacteria with an emphasis on methane biofilter systems
… of xmoCAB genes in methane, ethane and propane enrichment cultures from environmental samples. Single cell genomes were sequenced from the xmoCAB positive sorted cells of a propane enrichment culture. Screening the genomes identified Polaromonas and Rhodoferax as containing multiple xmoCAB …
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Studies on soil and microbial lipases
… A bacterial isolate obtained by enrichment culture, and designated Pseudomonas 017, was capable of producing high levels of extracellular inductive lipase when grown upon triacylglycerols, long-chain fatty acids and hydrocarbons. Growth upon these substrates was accompanied by the …
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