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Showing 1 to 15 of 15 for “"enolase superfamily"”.
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Discovery of Function in the Enolase Superfamily
… are incorrectly annotated. We are using the enolase superfamily as a model system to try to solve that problem. To date, >4000 members have been identified in the enolase superfamily, of which ∼50% have unknown functions. The muconate lactonizing enzyme (MLE) subgroup contains a family of …
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Discovery of function in the enolase superfamily
… are incorrectly annotated. We are using the enolase superfamily as a model system to try to solve that problem. To date, >4000 members have been identified in the enolase superfamily, of which ~50% have unknown functions. The muconate lactonizing enzyme (MLE) subgroup contains a family of …
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Natural and Engineered Promiscuity Within the Enolase Superfamily
The dehydration of both D-mannonate, via syn-elimination, and D-altronate, via anti-elimination, is analogous to the stereochemical promiscuity engineered into the GlucD active site. The redesign of GlucD and the characterization of RspA illustrate the same type of promiscuity: syn and anti …
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Galactonate Dehydratase: Exploring the Frontiers of the Enolase Superfamily
The crystal structure of GalD indicates that His 185 may serve as a general acid catalyst to facilitate the departure of the 3-OH leaving group and/or stereospecifically protonate the product in the subsequent tautomerization reaction. The function of His 185 was investigated by comparing the …
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The Identification of Novel Enzymatic Functions in the Enolase Superfamily
… the functional and catalytic diversity in the enolase superfamily is a valuable enterprise in accelerating the field of genomic enzymology. This is of paramount importance as the accumulation of genomic data is threatening to outstrip the ability for biochemists to study proteins at a pace that …
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Functional and Mechanistic Investigations of Enzymes in the Enolase Superfamily
In this thesis, the enolase superfamily is traced phylogentically and studied in detail to ascertain which active site residues are necessary to maintain function, kinetic competence, and/or substrate specificity. Methods for understanding the details of many enzymes in the enolase superfamily are …
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Functional and physiological discovery in the mannonate dehydratase subgroup of the enolase superfamily
… the mannonate dehydratase subgroup (ManD) of the enolase superfamily (ENS). The outcome affirms the dangers of homology-based annotations while discovering novel metabolic pathways. Furthermore, the experimental verification of these pathways (in vitro and in vivo) has provided a platform to test …
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Divergent Evolution of Enzymatic Activity: Functional Assignment and in Vitro Evolution in the MLE Subgroup of the Enolase Superfamily
The mechanistically-diverse members of the enolase enzyme superfamily provide an opportunity to understand generalized strategies for the divergent evolution of new enzymatic function, particularly within the context of enzymes containing the ubiquitous (beta/alpha)8-barrel fold. We have used the …
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Functional assignments in the enolase superfamily: investigations of two divergent groups of D-galacturonate dehydratases and galactarate dehydratase-III
… well as mechanistic features. To this end, the enolase superfamily is an excellent model system for functional assignment because more than half of the members still lack functional identification. Structurally, these enzymes contain substrate specificity residues in the N-terminal capping …
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Mining the enolase superfamily for new functions: investigations of D-glucarate dehydratase related proteins (GlucDRP) and L-lyxonate dehydratase proteins (LyxD)
… Nevertheless, annotating the homologs within a superfamily is a valid approach. To this end, the enolase superfamily is an excellent model system for functional assignment. Structurally, this superfamily contain substrate specificity residues in the N-terminal capping domain and catalytic …
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Mechanistic Investigations of Mandelate Racemase: The Electrophilic Catalysts
MR is a member of the enolase superfamily (Babbitt et al., 1996), which is a family of proteins which are both structurally and mechanistically related. All the family members have highly conserved metal ion ligands. Based on the homology to enolase, which requires two divalent metal ions, MR as …
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Characterization of Ortho-Succinylbenzoate Synthase (Osbs): A Study of Mechanism, Proficiency and Evolutionary Diversity
… synthase (OSBS), an enzyme of the enolase superfamily, catalyzes a dehydration reaction in the menaquinone biosynthetic pathway. Menaquinone is an electron acceptor that is utilized in bacteria, archaebacteria and red green algae. OSBSs from over 70 organisms have been identified …
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Assignment of Enzyme Function Through Characterization of the RuBisCO and Enolase Superfamilies
The second superfamily explored in this work is that of the enolase superfamily. Genomic context and primary amino acid sequence make it clear that although the enzymes in this superfamily are structurally and mechanistically related, the chemistry and substrates are varied. Two highly divergent …
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Study of Enzyme Evolution Within the MLE Subgroup Focusing on Characterizing Member Enzymes for the Purpose of Discovering Relationships Among Sequence, Structure, and Function
… used to develop catalysts for new reactions. The enolase superfamily possesses the most ubiquitous protein fold in Nature while the muconate lactonizing enzyme (MLE) subgroup represents the most divergent in chemical reactions in the superfamily; therefore, the MLE subgroup is an excellent …
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Functional discovery in the oxidative D-galacturonate assimilation pathway and development of the enzyme similarity web tool
… and provides an example in which members of the enolase superfamily from Agrobacterium tumefaciens strain C58 are mined in a shotgun approach to discover novel enzymatic activities. In the second part of this work, combined bioinformatics and experimental approaches are used to identify two novel …