Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"enhancer trap"”.
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Towards a Molecular Genetic Analysis of Spermatogenesis in Drosophila
… in Drosophila. We generated a collection of enhancer trap lines that identify all cell types and stages of the developmental program. We used marker lines to show that somatic cell fates are altered in the absence of germ cells, suggestive of signaling events between germ line and soma during …
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The regulation of genes involved in trichome development
… development was discovered with the aid of an enhancer trap line with robust reporter gene expression in developing trichomes. Pharmacological studies indicate that this α-carbonic anhydrase may play a role in trichome expansion. SIAMESE (SIM) was first identified in a mutant screen in the …
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In Pursuit of a Molecular Fountain of Youth: The Identification and Characterization of Lifespan Regulators in Drosophila
… expression of UAS-Snz from the snz Gal4 enhancer trap insertion, active in metabolic tissues, rescued lifespan extension. Notably, old snz mutant flies remain active and fertile indicating that snz mutants have prolonged youthfulness, a goal of aging research. Since mammals have …
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Novel Approaches to Transgenesis in the Teleost Medaka (Oryzias latipes)
… methods allows for the first time efficient enhancer trapping in fish. First, a transposon-based approach using the artificially reconstructed Sleeping Beauty (SB) transposon was established. To address the potential of SB for transgenesis, microinjection experiments were performed. …
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Functional and Genetic Analysis of Choroid Plexus Development in Zebrafish
… possible in other model systems. By utilizing an enhancer trap line, Et(cp:EGFP) sj2 that expresses the enhanced green fluorescent protein (EGFP) in CPe, we have demonstrated that the zebrafish CP possesses barrier properties such as tight junctions, transporter activity, and regulating central …
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The role of Ultrabithorax negative autoregulation in Drosophila melanogaster
… and Ubx levels are high. We identify an enhancer that captures this regulation and identify a binding site for Ubx/Exd/Hth. In Chapter 3, we describe another function for Ubx negative autoregulation: promoting developmental robustness by buffering haltere size against changes in Ubx …