Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"enhancer elements"”.
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Evaluating the Conservation of RNA Packaging Enhancer Elements Across Lentiviruses
It was previously thought that the RNA structural elements within the 5’UTR were the sole determinants of RNA packaging in HIV-1. However, in 2013 Chamanian et al. discovered that the region overlapping the Ribosomal Frameshift Signal (RFS) acted to enhance RNA packaging 50 fold and was thus named …
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Sox2 co-occupies distal enhancer elements with cell-type-specific POU factors to specify cell identity in embryonic stem cells and neural precursor cells
Sox2 is a master regulator of two distinct cellular states, that of pluripotent embryonic stem cells (ESCs) and multipotent neural progenitor cells (NPCs), but what common or distinct roles Sox2 may play in these cell types not fully understood. Further, the molecular mechanisms by which Sox2 can …
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DISCOVERY OF NOVEL EPIGENETIC MECHANISMS OF CARCINOGENESIS BY GENOME-WIDE PROFILING OF NON-CODING REGULATORY ELEMENTS
… of the activity states of distal regulatory elements. Therefore we can use these epigenetic marks to study important regions of the cancer genome that are key regulators of transcription with unprecedented resolution and accuracy. In this work, in-depth studies of epigenetic features of both …
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Molecular cloning and analysis of the genes for cotton palmitoyl-acyl carrier protein thioesterase (PATE) and Δ-12 fatty acid desaturase (FAD2-3) and construction of sense and anti-sense PATE plasmid vectors for altering oilseed composition of transgenic cotton plants.
… bp was sequenced, with the potential promoter/enhancer elements including basic helix-loop-helix elements (E box). Alkaline blot hybridization of cotton genomic DNA suggests the presence at least two FatB1 thioesterase genes in cotton. Four plasmid constructs for both constitutive and …
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Albumin Transcriptional Regulation
… infection, the minimal rat promoter and mouse enhancer elements were identified, functionally dissected and shown independently to be hepatocyte specific elements. Based on these studies, multiple cis-acting DNA regulatory regions were elucidated and the cognate proteins which interact with …
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Analysis of DNA-binding Proteins in Yeast Saccharomyces Cerevisiae
… of multiple proteins with promoter and enhancer elements. A variety of approaches are currently used to study these interactions in vivo, in vitro as well as in silico. With the genome sequences of many organisms now readily available, a plethora of DNA functional elements have been …
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Evidence that ARNT plays a role in the regulation of the immunoglobulin heavy chain enhancer and identification of a putative ARNT ligand
… at the IgH (immunoglobulin heavy chain) enhancer. We were able to determine that ARNT appears to partially down-regulate activation at the IgH enhancer by Pan-1 in transient transfection assays by cotransfection of the multimerized murine form of the IgH enhancer elements 1-1E2, !-LE3 , …
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Characterization of cDNA and Genomic Clones for a Palmitoyl-acyl Carrier Protein Thioesterase and an Osmotin-Like PR5 Protein in Gossypium Hirsutum.
… genomic clone has two putative ethylene response elements (GCC boxes) found in other PR5 gene promoter regions, as well as several tentative promoter/enhancer elements possibly involved in spatial/temporal gene expression.
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Organ-Level Communication During Heart Regeneration In Zebrafish.
… a novel class of remote tissue regenerative enhancer elements (r-TREEs) responsible for remote gene activation during tissue regeneration. Interestingly, removing cebpd associated enhancer element CEN only abolished gene activation in remote uninjured brain and kidney but not local …
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Investigating the function of DNA G-quadruplex structures in human enhancers
… in diverse genomes, including promoters and enhancers. Enhancers interact with target promoters through 3D folding of interphase chromosomes to regulate gene transcription. However, the functional relevance of G4 structures at non- coding enhancer elements remains poorly understood. In this …
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HIF2a regulates ccRCC tumorigenesis through activation of cell cycle regulators
… such as MYC, CCND1 and TGFa. Furthermore, enhancer profiling performed through H3K27ac ChIP-seq demonstrated the activation of enhancer elements in the close vicinity of these genes. Moreover, HIF2α ChIP-seq showed that it bound the same enhancers suggesting that HIF2α interaction with …
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Unravelling the epigenetic landscape of pancreatic cancer: The role of cancer-associated fibroblasts in transcription factor regulation
… direct cell-cell contact. SOX2 was recruited to enhancer elements and mediated expression of genes associated with epithelial-mesenchymal transition and hypoxia. In an orthotopic in vivo model, co-injection of cancer cells with either iCAFs or myCAFs reduced survival compared to injection of …
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Examining the effect of CBP on the E2A-PBX1 and HOXB4 interaction
… that the addition of CBP interactive peptide elements encoded by E2A to PBX1 allows E2A-PBX1 to stabilize a ternary complex involving E2A-PBX1, HOX, and CBP resulting in the deregulated expression of critical PBX1 or HOX target genes. I demonstrate using in vitro protein-protein interactions …
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Histone Deacetylase 7 and Transcriptional Regulatory Networks of the Vascular Endothelium
… progress toward the identification of the enhancer elements driving the endothelial-specific expression pattern of HDAC7. Detailed characterization of this enhancer is likely to implicate new signaling pathways as being involved in the genetic regulation of vascular development and …
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Transcriptional and structural control of cell identity genes
… gene expression through binding to enhancer elements in the genome. These enhancer-bound transcription factors regulate genes by contacting target gene promoters via the formation of DNA loops. It is becoming increasingly clear that transcription factors operate and regulate gene …
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Role of the RUNX2 rs59983488 variant in bone mass determination and fracture risk in Malta
… vitamin D receptor (VDR) binding and several enhancer elements resulting in reduced gene expression and hence BMD. The aim of this study was to determine the genotype and allele frequencies of the RUNX2 rs59983488 variant in the Maltese population, and to investigate the effect of the variant …
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Genome-wide prediction of developmental enhancers and analysis of evolutionary plasticity in Drosophila
… 18 sequences have been constructed for embryonic enhancer prediction. Clustering of transcription factor binding sites is the traditional approach for cis-regulatory element prediction, but several drawbacks exist. In contrast to popular clustering approaches, my proposed method utilizes paired …
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Nuclear respiratory factor 1: a novel inhibitor of the human gene promoter of the cardiac isoform of acetyl-coenzyme a carboxylase
… containing multiple copies of USF1-specific enhancer elements (pUSF1-Luc). Results: NRF-1 overexpression reduced ACCl3 gene promoter activity by 56 ± 8.8% (p<0.001 vs. pPlll3-1317/+65-Luc). Cotransfection with the dnNRF-1 construct abrogated this effect. Addition of AICAR dose-dependently …
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Dissecting the role of CHD4 in the NuRD and ChAHP complexes
… which contributes to silencing of transposable elements (TEs). CHD4 generally functions to increase nucleosome density, however the specific chromatin remodelling mechanisms employed by NuRD and ChAHP to regulate gene expression and repress repetitive elements have not been fully characterised. …
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Control of Regulatory Element Function by Histone H3.3
… enriched at transcriptionally active regulatory elements such as promoters and enhancers. While recent studies have revealed a role for H3.3 in silencing repetitive elements and repressing developmentally regulated promoters, it is unclear how H3.3 contributes to chromatin states at active …
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