Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"engrailed"”.
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The engrailed homeodomain : determinants of DNA-binding affinity and specificity
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.
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Engrailed genes are cell autonomously required for the survival of the mesencephalic dopaminergic neurons
The dopaminergic neurons of the substantia nigra and ventral tegmentum are the main source of dopamine in the central nervous system. The progressive loss of dopaminergic neurons in the substantia nigra compacta over several decades leads to one of the most prominent human neurodegenerative …
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Characterization of Retinal Defects in Engrailed-2 Knockout (En2-/-) Mice, a Model for Autism Spectrum Disorders (ASD)
Mice lacking the homeobox-containing transcription factor En2 (En2-/- mice) are considered as a reliable animal model for investigating the neurodevelopmental basis of autism spectrum disorders, as they display some ASD-related anatomical deficits, including cerebellar hypoplasia (Joyner A L, et …
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Mitochondrial Instability, Lack of Erk1/2 Activation and High Expression of P75NTR in Engrailed-Deficient Mesencephalic Dopaminergic Neurons
… Disease. The homeobox transcription factors, Engrailed-1 and Engrailed-2, are essential for survival and long-term maintenance of this neuronal population. In mutant embryos, null for both genes, the mesDA neurons are generated and start to express their neurotransmitter phenotype, but fail to …
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Histone Demethylase LSD1 Restricts the Size of the Germline Stem Cell Niche in Drosophila Ovaries
… signal in the adult germaria. I have identified engrailed as a direct target of Lsd1 by performing Chromatin Immunoprecipitation (ChIP-seq) analysis in the escort cells of the Drosophila ovary. Engrailed is expressed in the cap cells of wild type germaria and in Lsd1 mutants engrailed transcripts …
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Odd-paired: a zinc finger pair-rule protein required for the timely activation of engrailed and wingless in Drosophila embryos
<p>Here I report a molecular and genetic analysis of the pair-rule gene odd-paired (opa). Genetically, opa is essential for the appropriate level and timing of en and wg expression in all parasegments, but opa activity alone does not determine the restricted spatial domains of these genes. I …
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Conditional Loss of Engrailed 1/2 in Rhombic Lip-Derived Neurons Increases Intrinsic Rhythmicity and Decreases Overall Variability of Eupneic Respiration
… loss of the developmental patterning gene Engrailed 1/2 in rhombic lip-lineage neurons and exhibit a proportional scaling-down of neuron number in hypoplastic lobules of the CRB. We utilized whole-body unrestrained plethysmography to measure respiration during eupnea, and evaluated the …
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Appendage development and early Distal-less regulation in arthropods: A study of the chelicerate Tetranychus urticae (Acarida)
… antero-posterior positioning genes wingless and engrailed, although one of the two engrailed paralogs I identified is reportedly expressed in posterior segmental compartments, consistent with possible conservation of Engrailed-Wingless interactions in metameric patterning and positive regulation …
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MICROMORPHOLOGY AND GENE EXPRESSION IN MUSCLE AND SHELL DEVELOPMENT OF THE MOLLUSCA
… and expression of the homeobox gene engrailed (en), in order to gain insights regarding the scaphopod-bivalve relationship. The results enable far reaching conclusions regarding the evolution and the phylogeny of the Mollusca. Solenogastres TEM analysis of adult Solenogastres revealed …
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Delimitating Species Boundaries within the Liomys Pictus- Spectabilis Complex (Rodentia: Heteromyidae)
… cytochrome b gene and two nuclear introns from engrailed protein (190 bp) and myosin heavy chain (254 bp) genes for approximately 712 individuals representing 82 localities across the range of L. pictus and L. spectabilis. Maximum parsimony, maximum likelihood, and Bayesian analyses suggest that …
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Patterning the Drosophila Embryonic Central Nervous System: Establishing Neuroblast-Specific Gene Expression and Identity
… of segment polarity genes---hedgehog, wingless, engrailed, and gooseberry---act in the neuroectoderm and in neuroblasts to regulate the spatial pattern of huckebein, a gene required for the proper development of identified neurons and glia. Along the dorso-ventral axis, the ventral nervous system …
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The guardians of cell fate: protective mechanisms that ensure proper cell fate and patterning during imaginal disc regeneration in drosophila
… at the wound misregulating the expression of engrailed. The other chapter will detail the identification of the pioneer transcription factor Zelda as being upstream of taranis expression during regeneration. Zelda is found to be expressed at the same place and time as Taranis, and reduction of …
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A Genetic Screen for Tribbles Suppressors Identifies the E3 Ubiquitin Ligase Neuralized as a Novel Target
… In the wing, expression of tribbles by the engrailed-GAL4 driver generates a distinct visual phenotype; larger cells in the posterior of the wing and overall smaller tissue size. Co-misexpression of the known targets of Tribbles slbo (Slow Border Cells) and string suppresses the Tribbles …
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Investigation of Interactions between Homeodomain Proteins and DNA
… von Bicoid- (Bcd), Ultrabithorax- (Ubx) und Engrailed- (En) Homeodomänen hat gezeigt, dass die Bindung dieser Proteine an doppelsträngige 15 bp Oligonukleotide Veränderungen in der Sruktur der Homeodomänen hervorruft. Diese Veränderungen sind unterschiedlich für Wechselwirkung mit …
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Identification and characterization of genes expressed by the midbrain dopaminergic neurons
… to show that the mDA neurons require the Engrailed (En) genes cell autonomously for their survival, and the over-expression experiment of En-1 suggested that the En genes are not required for the formation of mDA precursor cells. I identified more genes specifically expressed by the mDA …
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Role of Xenopus Pitx3 during early development.
… nerve, and diencephalon. Expression of a xPitx3/engrailed repressor chimera alters early expression domains of Pax6, Rx, and Six3, and later inhibits lens development consequently abrogating retinal induction. This later inhibition of eye development is reflected by diminished expression of Pax6, …
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The function of chromatin regulators during patterning and growth in Drosophila wing disc regeneration
… stabilizing the expression of the posterior gene engrailed. Thus, both SWI/SNF complexes are essential for proper gene expression during tissue regeneration, but they play distinct roles in regulating growth and cell fate. In addition to the SWI/SNF complexes, which belong to the Trithorax group …
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Regulation of segment polarity genes in the head region of different arthropods
… polarity genes wingless, hedgehog (hh) and engrailed in Drosophila and in a comparative approach also in the short-germ band red flour beetle Tribolium castaneum. During this study an intercalary-specific cis-regulatory element (ic-CRE) of Dm_hh was isolated, which further supports the …
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Investigating the cell surface code for compartment boundary formation in Drosophila embryos
… nascent mRNA transcripts under the control of an engrailed enhancer region. By combining this method with cell tracking, I demonstrated that in tartan null mutant embryos, both odd and even-numbered PSBs lose their straightness during axis extension. This indicates that Tartan is required for …
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The role of Decapentaplegic (Dpp) in Drosophila wing development
… signaling. We propose that the selector gene engrailed, Hh and Dpp signaling interact with each other to regulate target genes expression and thus to pattern the wing along the A/P axis. Further more, I showed that Dpp signaling is also participating in the patterning along the D/V axis by …