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Showing 1 to 1 of 1 for “"endpoint RT-PCR"”.
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Tracking Tobacco Mosaic Virus Infection from Infected Seeds to Seedlings Confirms Seed Transmission in Tobacco (Nicotiana tabacum L.)
… germination. Total RNA was extracted from each part and synthesized into cDNA for analysis. A quantitative real-time PCR (RT-qPCR) assay was used to determine TMV concentration of each sample. Endpoint RT-PCR was used to determine a conservative threshold value from the RT-qPCR results. These …