Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 175 for “"endosomes"”.
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Molecular Mechanisms of Inward and Outward Budding From Multivesicular Endosomes
<p>Regulating the residence time of membrane proteins (e.g. transporters, ion channels, receptors) on the cell surface can modify their response to extracellular cues and allow for cellular adaptation to environmental conditions. The fate of membrane proteins that are internalized from the plasma …
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Assessment of endosomes as sites of signal transduction in plants during bacterial attack
… is less well understood. Furthermore the role of endosomes in implementing these responses is also not well understood. Outstanding questions are the importance of signalling by proteins from locations other than the PM and the relevance this has to overall signal transduction and how do endosomes …
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Kinetic analysis of endosome processing : maturation of early endosomes and vesicular traffic to lysosomes
… of the endocytic contents markers within early endosomes that mature to form non-fusogenic late endosomes (maturation model). This maturation phase is followed by a segregation phase where intermingling of contents between vesicles no longer takes place. To establish the mechanism(s) involved in …
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In vitro reconstitution of Central Spindle Motility
… the asymmetric segregation of signalling endosomes containing fate determinants in only one daughter cell. A modest asymmetry in the density of microtubules within the antiparallel MT overlap of central spindle, with more MTs on the anterior side of the spindle, generates one order of …
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The Ubiquitin Ligase Ube4B Is Required For Efficient Epidermal Growth Factor Receptor Degradation
… fate. Membrane proteins initially enter early endosomes, and subsequently late endosomes/multivesicular bodies (MVBs), before being degraded in the lysosome. The MVB is a subset of late endosomes characterized by the appearance of small vesicles in its luminal compartment. These vesicles …
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Regulation of autophagosome formation and maturation by neurodegeneration-associated proteins
… protein 11A (RAB11A)-positive recycling endosomes. Autophagosomes are closed by the endosomal sorting complex required for transport (ESCRT) complex and then released by dynamin 2 (DNM2) before fusing with lysosomes to enable the degradation of the autophagy substrates. Impaired …
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Rab-domain dynamics in endocytic membrane trafficking
… to mediate trafficking between Golgi/TGN and endosomes instead.In order to address the dynamics of Rab-domains, fluorescently tagged Rab-GTPases were imaged during cargo transport reactions in living cells. Herefore high-speed/long-term imaging procedures and novel computational image analysis …
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Regulation of polarised trafficking by Klp98A
… Klp98A biases the motility of signalling endosomes towards the posterior daughter cell. Since these endosomes contain the cell fate determinants Notch and Delta, this contributes to an asymmetric cell fate. Mechanistically, Klp98A-mediated transport is achieved along the microtubule-based …
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Transport of soft materials in crowded environment
… Systems studied are actively transported endosomes in living cells, and diffusing semi-flexible polymer chains in rigid networks. By going beyond traditional particle tracking and trajectory analysis, it was discovered that for protein transportation rather than directing the …
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Somatostatin Receptor 2 Regulation By C-Terminal Motifs and Pdz Domain Proteins
… We determined that sst2 traffics from early endosomes to late endosomes and then on to the trans-golgi network (TGN). Trafficking from late endosomes to the TGN is dependent upon an intact C-terminal tail and the retromer complex of endosomal coat proteins. Additionally, removing either 3 or …
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Vps1, a Novel Recycling Factor For the Traffic of Early Endosome to Late Golgi
… in the recycling traffic of GFP-Snc1 from early endosomes to the late Golgi. In the absence of Vps1, I observed an aberrant accumulation of GFP-Snc1 puncta in the cytoplasm that I identified as early endosomes. The N-terminal GTPase and the C-terminal GED domains of Vps1 are essential for Vps1's …
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Optimization of organelle fractionation methods for quantitative analysis of gene delivery trafficking kinetics
… to the Horseradish Peroxidase assay, late endosomes and lysosomes consistently localize to fractions 11-13 and early endosomes in fractions 18 to 21. There was minimal labeling in fractions 14 through 17 demonstrating that separation of the organelles was achieved. With this higher …
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Axonal Translation and Links to Neuropathies
… translation can occur on Rab7a-associated late endosomes, where mRNA recruitment and translation are coordinately regulated. Notably, mRNAs encoding mitochondria-related proteins are found translating on late endosomes docking in the vicinity of mitochondria, suggesting late endosomes act as …
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Yeast Dynamin Functions with Clathrin at the Golgi
… endocytosis, but also traffic from the Golgi to endosomes. In this study, the interdependency between Vps1 and clathrin was investigated. Subcellular localization of clathrin was at the Golgi, early and late endosomes, implicating clathrin in multiple trafficking pathways within the cell. Vps1 …
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Functional Connection Between Yeast Dynamin and Retromer at the Endosome
… coat proteins for vesicles emerging from late endosomes. Loss of Retromer function has been previously implicated in both Parkinson's and Alzheimer's disease. Vps1, a yeast dynamin-like protein, plays a role in intracellular trafficking. Vps1 has been shown to localize at the endocytic sites to …
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Identification and Characterization of Effectors/Binding Molecules for the Small GTPase Rab15
… receptor internalization into early endosomes. Here internalized receptors are\r\nsorted for degradation in lysosomes, direct recycling back to the cell surface or indirect\r\nrecycling via a second recycling compartment called the pericentriolar recycling\r\nendosome. Rab GTPases …
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Deciphering The Role of Huntingtin In Endosomal Functions
… endosomal pathways. Upon endocytosis into early endosomes regulated by Rab5, the endocytosed cargo can either be recycled back to the plasma membrane (via Rab4/Rab11) or degraded through the endosomal-lysosomal pathway (via Rab7). Disturbances in normal Rab functions could lead to abnormal cargo …
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FMF assay for assessing vaccine generated antibodies in a biomimetic manner
… responsible for virus membrane fusion in the endosomes. In order to study Influenza vaccine response in a more biomimetic manner and overcome the deficiencies of the traditional functional assays, we developed a fluorescent membrane fusion assay (fMF). The assay uses viruses labeled with …
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PROTEINi screens identify ORP9 as a novel regulator of KRAS-driven signalling
… target organelles, such as the Golgi and late endosomes. ORP9 lipid cargoes include phosphatidylserine and cholesterol, which can be transferred out of the ER membrane in exchange for PI4P, which is then dephosphorylated by the ER-resident Sac1 phosphatase. TAT-OPX and TAT-HPX caused …
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Experimental and modeling studies of endosomal sorting using the epidermal growth factor/receptor system in fibroblasts
… membrane-associated receptors are sorted within endosomes and how this process affects ligand sorting.
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