Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 18 of 18 for “"endosomal trafficking"”.
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Endosomal Trafficking and Exosomal Secretion of Egfrviii
… results reveal novel aspects of the endocytic trafficking of EGFRvIII that underlie its reduced degradation and the mechanism by which it is packaged into exosomes for secretion. Moreover, I show that EGFRvIII secretion on exosomes can facilitate changes in the tumor microenvironment to enable …
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Molecular basis of cargo sorting by endosomal trafficking machinery
… to their intended final destination. The early endosomal system serves as a checkpoint within the cell marking a critical junction between the Golgi apparatus, plasma membrane and endo-lysosomal pathway. It is at these early endosomal organelles that the sorting of a transmembrane protein …
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Endosomal Trafficking as a Determinant of Antifungal Tolerance in the Pathogenic Fungus Candida albicans
… vacuole, and determine if, in turn, vacuolar trafficking influences azole susceptibility. Profound fragmentation of the C. albicans vacuole was observed as an early consequence of azole treatment, before significant growth inhibition was observed. Using a genetic approach, we determined that …
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Ubiquitin Modulates Tollip's PtdIns(3)P Binding and Dissociates the Dimeric State of C-Terminal Cue Domain
… signaling pathway and an adaptor protein for endosomal trafficking. We have demonstrated that the central C2 domain of Tollip preferentially interacts with phosphoinositides with moderate affinity. Remarkably, we found that ubiquitin modulates Tollip's lipid binding. We have observed an …
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The Role of Vps54 in Drosophila melanogaster Neuronal Development and Age Progressive Neurodegeneration
… while additionally interacting with differing endosomal trafficking components associated with disease phenotypes.</p>
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Structural Functional Investigation of HAP40
… dissected HAP40’s role in HTT stabilization, endosomal trafficking, and neurodegeneration. Structural modeling revealed remarkable conservation of the HTT-HAP40 complex across species, and functional studies demonstrated that deletion of the BΦ motif does not impair HAP40’s ability to …
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Trafficking motifs in potassium channels
… is of fundamental importance to investigate the trafficking mechanisms that regulate the surface density of these channels. Techniques in cell biology, molecular biology and biochemistry were employed to identify the molecular basis of Sar1-GTPase dependent ER exit of the KATP and hERG channels …
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Deciphering The Role of Huntingtin In Endosomal Functions
… Ras superfamily of small GTPases, govern cargo trafficking through endosomal pathways. Upon endocytosis into early endosomes regulated by Rab5, the endocytosed cargo can either be recycled back to the plasma membrane (via Rab4/Rab11) or degraded through the endosomal-lysosomal pathway (via …
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A Role for the Phosphoinositide Lipid Kinase PI4KIIIbeta in Breast Oncogenesis and Akt Activation
… regulating PI3K/Akt signalling through Rab11 and endosomal trafficking.
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Dynamic subcellular proteomics identifies novel regulators of adipocyte insulin action
… GOLP3) and components of the endolysosomal trafficking and mTORC1 regulatory pathways. Our unbiased whole-cell proteomics approach revealed the plasma membrane as the most prominent site of insulin-responsive proteome remodelling. To further investigate these insulin- driven changes, we …
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Regulation of synaptic structure and function at the Drosophila neuromuscular junction
… my studies on how regulation of endocytic trafficking controls synaptic growth during neuronal development. To identify regulators of synapse assembly, I carried out a large-scale EMS mutagenesis screen of the second chromosome. From this screen I identified a mutation in actin 57B that …
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Functional and Mechanistic Analysis of Protein Degradation by Human Cytomegalovirus to Uncover Viral Immune Evasion Mechanisms
… with the vacuolar-type H+-ATPase to regulate endosomal acidification, with implications for endosomal trafficking, autophagy, immune signalling and many other cellular processes. The viral gene responsible for downregulation of DMXL1 was identified as pUS33A, which may recruit the E3 ligase …
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A New Approach To Identifying Effectors and Regulators of Small GTPases
… Ras superfamily are major regulators of membrane trafficking and signalling, ensuring specificity and efficiency through their spatiotemporal regulation.Identifying the proteins interacting with small GTPases is vital for understanding how the small G proteins are regulated and where they …
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Studies on the Roles of PDGFRA and EGFR in the Classification and Identification of Therapeutic Targets for Human Gliomas
… and result in deviation of PDGFRA from endosomal trafficking and recycling compartment to the Golgi network in a reversible, dose- and time-dependent manner without affecting total PDGFRA expression. U0126 mediated down-regulation of PDGFRA surface expression correlated with diminished …
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Structural and Functional Basis for the Autoregulation of the Adaptor Protein TOM1
Target of Myb 1 (TOM1) is an endosomal adaptor protein that plays a role in cargo membrane trafficking for degradation by serving as an alternative endosomal sorting complex required for transport component. TOM1 has also been shown to serve as a novel phosphatidylinositol 5-phosphate (PtdIns5P) …
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Shedding light on the molecular interactomes of fast, complex biological processes using multispectral imaging with uncompromised spatiotemporal resolution
… complex biological processes, for instance, endosomal receptor sorting. This process occurs within a few seconds and is regulated by tens, or hundreds, of components on the endosomal membrane. Furthermore, these endosomes themselves move on the µm/sec range in 3D within the cell, making this …
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Primordial imperatives and the evolution of control of endolysosomal trafficking
Lysosomes solve fundamental obstacles to the evolution of complex life forms. Lysosomes sense and maintain cellular metabolic stores and ensure their judicious use. More importantly, lysosomes respond to changes in osmotic conditions to maintain the membrane, which protects life from the harsh …
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Binding properties of adaptor proteins Tollip and Tom1
… (PtdIns(3)P)-containing endosomal membranes. Tom1 and Tollip interaction and association with endosomes is proposed to be involved in the lysosomal degradation of polyubiquitinated cargo. Through cellular, biochemical, and biophysical techniques, we have further …