Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"endosomal acidification"”.
-
Processing of Exogenous Hen Egg-White Lysozyme for Presentation on Major Histocompatibility Complex Class I and II Molecules
… of HEL in liposomes targeted to early or late endosomal compartments. HEL in either early or late endosomal liposomes was presented on MHC class II; in contrast, only HEL in early endosomal liposomes was cross-presented on MHC class I. MHC class II presentation required nascent MHC molecules …
-
Functional and Mechanistic Analysis of Protein Degradation by Human Cytomegalovirus to Uncover Viral Immune Evasion Mechanisms
… with the vacuolar-type H+-ATPase to regulate endosomal acidification, with implications for endosomal trafficking, autophagy, immune signalling and many other cellular processes. The viral gene responsible for downregulation of DMXL1 was identified as pUS33A, which may recruit the E3 ligase …
-
Comparative analysis of cytotoxic necrotizing factor (CNF) toxins: Compatibility of cargo with delivery vehicle and identification of amino acid residues that modulate pH-dependent cytosolic cargo delivery
… differential sensitivity of the CNF toxins to endosomal acidification impacts their cargo delivery efficiency. We found that replacing particular acidic amino acid residues from the putative insertion-trigger motif of the CNFy translocation domain with those in CNF3 promotes endosomal escape at …
-
Understanding the biological barriers to Jurkat Transfection using non-viral peptides
… of cationic nanoparticles. Besides, eliminated endosomal acidification in Primary T-cells limits the endosomal escape of transfection agents based on pH sensitiveness. <br/><br/>Next, RALA and RALA-Ks variants were designed and characterised for overcoming identified barriers to T-cell …
-
Investigation of the intracellular trafficking pathways of the dermonecrotic toxin family
… of PMT is dependent on its transfer to late endosomal compartments. In contrast, disruption of Golgi-endoplasmic reticulum (ER) trafficking with brefeldin A (BFA) increased iii PMT activity, suggesting that inhibiting PMT trafficking to non-productive compartments that do not lead to …
-
FMF assay for assessing vaccine generated antibodies in a biomimetic manner
… in the endosomes. The latter was tested using endosomal acidification inhibitor Bafilomycin a1 which blocked membrane fusion by 85%. Specificity of the assay was tested using two different subtypes of viruses H1N1 (A/Puerto Rico/8/1934 and A/Brisbane/59/2007), and H3N2 virus (A/Aichi/68) with …
-
Molecular and Cellular Mechanisms Responsible for Low-grade Stress and Inflammation Triggered By Super-low Dose Endotoxin
… In the presence of low-dose LPS (50pg/ml), endosomal-lysosomal fusion is inhibited and a loss of endosomal acidification required for the successful clearance of cellular debris and resolution of inflammation was observed. Additionally, super-low dose LPS induced the accumulation of p62 …
-
Influenza A virus infection and innate immunity in an established murine alveolar-like macrophage model
… in MPI macrophages as epithelial cells. With endosomal acidification facilitating HA fusion to the endosome to allow viral RNP escape. Upon detection of IAV by the macrophage three distinct immune response phases occur, two ‘early’ before RNA replication and one ‘late’ after replication. These …
-
Studies on the mode of action of dermonecrotic toxins: Effects on adipogenic signaling pathways and delivery of c-terminal cargo into the cytosol
The dermonecrotic toxin family consists of the dermonecrotic toxin from Bordetella spp. (DNT), the cytotoxic necrotizing factors from Escherichia coli (CNF1, CNF2 and CNF3) and Yersinia pseudotuberculosis (CNFY), and the mitogenic toxin from Pasteurella multocida (PMT). These relatively large …