Global ETD Search
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Showing 1 to 20 of 22 for “"endoribonuclease"”.
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Endoribonuclease toxin-antitoxin systems in bacteria : targets and growth inhibition
… The toxic element of many of these systems is an endoribonuclease. In this work, I characterize the RNA targets of 9 endoribonuclease toxins encoded by the bacterium Escherichia coli.
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Mechanistic insights into endoribonuclease activation of the mRNA 3' end processing machinery by Cleavage Factor IA
… module and the phosphatase module. The endoribonuclease within CPF is held in an inactive form and must be activated to maintain fidelity. The specific mechanism of activation remains unknown, but it is thought that the endoribonuclease requires additional protein factors within CPF and …
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Ire1 regulates cyp11a1 transcription to mediate steroidogenesis in the ovary
… expression, we sought to determine if IRE1 endoribonuclease activity is required for CYP11A1 expression. KGN cells were treated with and without forskolin, or tunicamycin and co-treated with the IRE1 endoribonuclease inhibitors MKC-4485 and MKC-3946. MKC-4485 and MKC-3946 potently inhibited …
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Regulatory Mechanism of the RNAi Pathway
… (RISC), wherein single-stranded siRNA guides the endoribonuclease Argonaute (Ago) to catalyze sequence-specific cleavage of complementary mRNA. It remains unclear as to what constitutes holo-RISC, how is RISC assembled and how is RISC regulated. Here we took a candidate approach to reconstitute …
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Endoribonukleazių poveikio ankstyviesiems bakteriofago T4 transkriptams tyrimas /
… yet to be investigated. Apart from the impact of endoribonuclease RegB, no other effects of Escherichia coli- or phage-encoded proteins are known to be involved in the degradation of early mRNAs. This study has aimed to identify E. coli endoribonucleases that are involved in secondary processing …
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Towards Understanding Helicase and Chaperone Activities in the RNA Degradosome
… components of the RNA degradosome include the endoribonuclease RNase E, the DEAD-box RNA helicase RhlB, the glycolytic enzyme enolase, and the phosphorolytic exoribonuclease PNPase. The degradosome has also been previously shown to associate with the RNA chaperone Hfq to form a small RNA guided …
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Temporal gene expression and regulation in T4 phage
… and new potential cleavage sites for the T4 endoribonuclease RegB. At the translational level, we identified many instances of previously unreported changes in translational efficiency over the course of infection, indicating the presence of intricate and uncharacterized mechanisms of …
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Biophysical characterization of the Escherichia coli RNA degradosome
… components of the RNA degradosome consist of the endoribonuclease RNase E, the DEAD-box helicase RhlB, the glycolytic enzyme enolase, and the exoribonuclease PNPase. The main scaffold component of the RNA degradosome, RNase E, contains an amphipathic alpha helix that recruits the degradosome to …
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Nucleoid condensation in Escherichia coli by the DNAbinding protein SymE
… inner membrane pores, SymE is believed to be an endoribonuclease that has predicted structural similarity to DNA binding proteins. In this work I sought to better understand SymE’s target preference and specificity. Surprisingly, when I assessed the transcriptome for RNA cleavage events after …
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The Domains of The Catalytic Subunit of The Eukaryotic Rna Degrading Exosome, Rrp44P, Have Distinct Functions
… domain. Specifically, the PIN domain exhibits endoribonuclease activity in vitro and is essential for exosome function. Further analysis of the nuclease activities of Rrp44p indicate a role for the exoribonuclease activity of Rrp44p in the cytoplasmic and nuclear exosome. This work has also …
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Regulation of Metabolism in Mycobacteria and Cyanobacteria
… that VapCslr1210 has metal dependent endoribonuclease activity that can be inhibited by VapBslr1209. Markerless deletion strains of five Synechocystis vapBC operons were made and growth experiments of three of the deletion strains were conducted. Growth experiments identified a reduced …
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Exploring the role of the unfolded protein response in C. elegans neurons
… factor that acts downstream of the UPRER kinase/endoribonuclease IRE-1, in the nervous system of C. elegans increases the lifespan and healthspan of worms through UPRER induction in the intestine cell non-autonomously. To investigate XBP-1s-dependent changes in the nervous system of these …
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The role of a toxin-antitoxin system in the arms race between bacteria and phage
… system called toxIN, containing the endoribonuclease toxin ToxN and the short, repetitive RNA antitoxin toxI, in the arms race between phages and bacteria. Using RNA sequencing to track ToxN activity during T4 infection, I show that, following phage infection, ToxN is liberated from …
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A STRUCTURAL AND FUNCTIONAL ATLAS OF THE HUMAN RIXOSOME COMPLEX
… along with enzymatic members including LAS1L (endoribonuclease), NOL9 (RNA kinase), SENP3 (SUMO protease), and MDN1 (AAA+-ATPase motor). The rixosome also has conserved RNA processing functions at heterochromatin, effectively triggering polycomb gene silencing. It has become increasingly clear …
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Post-Transcriptional Regulation of Virulence Genes by GlmY and GlmZ in Enterohemorrhagic E. Coli
… of the operon from the rest that requires the endoribonuclease RNase E. Overexpression of either sRNA results in the downregulation of the latter fragment of both the LEE4 and LEE5 operons. In the case of LEE4, this is through direct binding of GlmZ to a region within the LEE4 mRNA. We also …
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Molecular bases for modulation of host cellular responses by porcine reproductive and respiratory syndrome virus non-structural protein 11
… as NendoU, and this domain is known to have a endoribonuclease activity. This RNase activity is important but not essential for viral replication in equine arteritis virus (EAV), suggesting that Nsp11 may target cellular RNAs and thus may function to counteract the host’s antiviral response. By …
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Characterization of ToxINPa-sensitive bacteriophages of Serratia sp. ATCC 39006
… atrosepticum. ToxINPa consists of an endoribonuclease toxin and RNA antitoxin and is inactive as a heterohexameric complex until infection by certain phages causes destabilization of ToxINPa, leading to bacteriostasis and, eventually, lethality. However, it is still unknown why only …
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Synchrony of harmony and chaos in a dynamic, bacterial riboregulatory machine
… of the assembly is RNase E, a homotetrameric endoribonuclease. Each monomer of RNase E can be divided into two domains: the N-terminal domain which carries out the catalytic activity, and the C-terminal domain, which is intrinsically disordered but punctuated by microdomains that correspond to …
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The Roles of Rnt1 and Putative Endoribonucleases in Eukaryotic mRNA Degradation
<p>Endoribonucleases initiate degradation by cleaving RNAs internally. Mutations in these enzymes have been shown to cause diseases such as cancer, developmental disorders, and neurodegenerative diseases. Yet, eukaryotic endoribonucleases have not been well studied, especially with regard to their …
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Functional genomic analysis of cell cycle progression in human tissue culture cells
… of libraries of highly complex pools of endoribonuclease-prepared siRNAs (esiRNAs). The analysis of the silencing efficiency and specificity of esiRNAs and siRNAs revealed that esiRNAs are as efficient for mRNA degradation as chemically synthesized siRNA designed with state-of-the-art …
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