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Showing 1 to 20 of 174 for “"endonuclease"”.
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Flap Endonuclease 1 ensures telomere replication and stability
Telomeres, protein-DNA structures that distinguish the end of a chromosome from a bona fide DNA double strand break, are integral to genomic stability. High fidelity replication of telomeres is indispensable for their stability. Telomere replication is a challenging task that is completed through …
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Structure and Function Studies on tRNA Splicing Endonuclease
… processing step is performed by tRNA splicing endonuclease (TSEN), a highly conserved heterotetramer with two catalytic and two structural subunits. In addition to this conserved function, archaeal TSEN also processes pre-rRNA and yeast TSEN cleaves some mRNAs that encode mitochondrial proteins …
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5-hydroxymethylcytosine and endonuclease G as regulators of homologous recombination
… vertebrate HR via interaction with the protein endonuclease G (EndoG). In this dissertation, I use biochemical/biophysical methods to elucidate the interaction between 5hmC and EndoG, thus working towards understanding their roles as regulators of recombination. I find that 5hmC forms a unique …
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Endonuclease processing in the rif region of the Escherichia coli chromosome
Endonuclease processing of mRNA derived from the rif region near 90 minutes on the Escherichia coli chromosome was examined. This region contains the secE-nusG and the rplKAJL-rpoBC (ribosomal protein) operons and it is thought that endonuclease processing by RNase E and RNase III plays some role …
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Characteristics of an Endonuclease Associated with Adenovirus Types 2 and 12
… sedimenting DNA is formed by a presynthesized endonuclease. Extracts of cells infected with adenovirus contain the endonuclease activity. The endonuclease appears in the cells as both an early enzyme and a late enzyme. The endonuclease which appears early after infection correlates directly …
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Studies on the Role of Endonuclease-I in Colicin-E2 Killing
Made available in DSpace on 2014-12-10T23:24:28Z (GMT). No. of bitstreams: 1 7317101.pdf: 4847136 bytes, checksum: 539972cf25e874c3aa928980fa00fafa (MD5) Previous issue date: 1972
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Functions of The Trna Splicing Endonuclease and Other Adventures In Rna Processing
<p>The tRNA splicing endonuclease (TSEN), has been studied for over three decades for its function in tRNA splicing. However, this enzyme has other functions that are just beginning to be characterized. Mutations in TSEN cause the neuronal disease pontocerebellar hypoplasia (PCH) that is …
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Analysis of nuclear transport signals of the human apurinic/apurimidinic endonuclease (APE1/REF1)
… signal (NLS) in human apurinic/apyrimidinic endonuclease (APE1), a key protein in both DNA base excision repair and transcriptional regulation, has not been analyzed in detail. We examined the role of specific residues in nuclear translocation of APE1, using green fluorescent protein (EGFP) …
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Sequence-specific recognition of DNA by RSRI endonuclease of Rhodobacter sphaeroides, an isoschizomer of EcoRI
In order to study the interaction of RsrI endonuclease with its DNA recognition sequence and to determine whether RsrI recognizes DNA using a mechanism similar to that of EcoRI, I have purified milligram amounts of the enzyme to near homogeneity from its natural source, Rhodobacter sphaeroides …
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Targeted Inactivation of APE1 Endonuclease by E96A Mutation Sensitizes Pancreatic Ductal Adenocarcinoma to DNA-Damagin Therapy
… for tumor survival. Apurinic/apyrimidinic endonuclease 1 (APE1/Ref-1) is a multifunctional protein with critical roles in base excision repair (BER) and redox regulation of transcription factors. This dissertation tests the hypothesis that selective disruption of APE1 endonuclease activity …
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Investigations into the mode of action of the DNA uridine endonuclease Mth212 of Methanothermobacter thermautotrophicus ΔH
Die spontane hydrolytische Desaminierung von Cytosin zu Uracil ist eine der häufigsten DNA-Modifikationen während des zellulären Stoffwechsels. Um dieser mutagenen Wirkung zu widerstehen, entfernen die meisten Organismen Uracil-Reste aus der DNA mittels Basen-Exzisionsreparatur (BER). Diese wird …
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Studies into the structural basis of the DNA uridine endonuclease activity of exonuclease III homolog Mth212
Mth212, ein Exonuclease III Homolog aus dem Archeon Methanothermobacter thermoautotrophicus, kompensiert das Fehlen einer DNA-Uracylglycosylase (UDG), indem es einen einzelstrangbruch auf der 5 -Seite des Uridinrestes in DNA katalysiert. Diese Reaktion ersetzt die Basenexzisions Reparatur (base …
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Changes in Mammalian Chromatin Structure as a Function of Protein-Poly(ADP-Ribosyl)ation by Endonuclease Digestion
… a Function of Protein-poly(ADP-ribosyl)ation by Endonuclease Digestion. Master of Science (Biochemistry and Molecular Biology), June 2004. 66 pages, 12 illustrations, Bibliography, 45 titles. Mammalian chromatin was exposed to either Deoxyribonuclease I or Micrococcal Nuclease digestion as a …
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The Development of Novel Apurinic/Aprymidinic Endonuclease/Redox-factor 1 Inhibitors for the Treatment of Human Melanoma
<p>Apurinic/apyrimidinic DNA repair endonuclease-1 (APE1), first recognized as an important DNA excision repair enzyme, is also known as Redox Factor-1 (Ref-1) involved in the activation of many nuclear transcription factors in both redox-dependent and independent manner. It has been …
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In vitro reconstitution of the human pre-mRNA 3′ end processing machinery and mechanistic insights into endonuclease activation
… remains limited. CPSF is an inherently inactive endonuclease on its own and was believed to require additional protein factors for its activation, enabling tight regulation of 3′ cleavage. To gain insight into the activation mechanism of the 3′ endonuclease, a minimal in vitro system with a …
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The role of the human endonuclease VIII-like 1 and 3 in the repair of DNA replication blocking lesions
The endonuclease VIII family of DNA glycosylases initiate repair of oxidative DNA base damage through BER and have been shown to resolve DNA inter-strand crosslinks (ICLs), arising from exogenous agents. Human NEIL1 (hNEIL1) activity on psoralen generated mono-adducts (MAs) and three-stranded DNA …
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Defining the role of endonuclease VIII-like 1 and 3 in the repair of interstrand crosslinks in cancer cells
… (ICLs). More recently, two DNA glycosylases (endonuclease VIII – like 1 & 3 [NEIL1 & NEIL3]) that excise oxidised bases from DNA, thus initiating base excision repair, have been found to participate in the resolution of psoralen induced ICLs. Therefore, this project set out to determine …
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Structural Analysis of Dna Complementary to Bovine Parathyroid Hormone Messenger-Rna and Restriction Endonuclease Mapping of the Parathyroid Hormone Gene
To facilitate studies on the structure and regulated expression of the gene for parathyroid hormone (PTH), full length single and double stranded DNA complementary (cDNA) to bovine PTH mRNA was synthesized by reverse transcriptase and E. coli DNA polymerase I. Single stranded cDNA migrated as a …
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