Global ETD Search
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Showing 1 to 12 of 12 for “"endolysin"”.
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Characterization of the Bacteriophage Felix O1 Endolysin and Potential Application for Salmonella Bioremediation
… to use bacteriophages, or the purified holin and endolysin proteins that make them lethal and lytic, as antimicrobial food treatments or additives. The bacteriophage Felix O1 is a promising candidate for development as an anti-Salmonella food treatment. This dissertation describes the work done to …
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Production and characterization of a phage endolysin with putative antimicrobial activity against Cutibacterium acnes
… to be developed to treat acne. One option is endolysins, highly conserved enzymes from bacteriophages that disrupt the bacterial cell wall. Endolysins retain bactericidal and bacteriostatic activity when applied to bacteria in vitro and are safe for topical application. We hypothesize that the …
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Bacteriophage Endolysins: Anti-Infectives and Lysidiagnostics for the Post-Antibiotic ERA
… such novel approach, the use of bacteriophage endolysins as diagnostic (lysidiagnostics) and therapeutic (enzybiotics) tools for pathogens of human disease. Endolysins are modular proteins that facilitate release of progeny phage by digesting the bacterial host cell wall. Structurally, …
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Functionalization of phage endolysins to treat infections of spore-forming Clostridia bacteria in the era of antibiotic resistance
… and efficacy against *Clostridia*, yet endolysin domains were the ultimate focus for host range analyses and subsequent experimentation. Finally, the feasibility of combining endolysin binding domains and antibacterial proteins was investigated for improvements in lytic and binding …
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In vitro model development for novel prevention measures against foodborne pathogens
… means of killing L. monocytogenes, bacteriophage endolysins were identified to have considerable potential for overcoming limitations of other antimicrobials in foods such as fresh cheeses. Endolysins are peptidoglycan hydrolases that lyse bacteria as part of the bacteriophage lytic cycle. They do …
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Improving the efficacy of nisin to control Listeria monocytogenes in queso fresco
… synergy of nisin with the Listeria phage endolysin PlyP100 against Listeria in QF was evaluated. By combining the commercial preparation of nisin with the purified endolysin PlyP100 in QF, the treatment reduced the viable populations of the pathogen to below the detection limit of …
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Investigating the key components of the chitinase secretion system in <i>Serratia marcescens</i>
… and <i>chiX </i>genes encode putative holin and endolysin proteins, respectively, and these are essential for secretion of chitinases. Using X-ray crystallography, the structure of full-length ChiX was resolved to 1.34 Å, revealing structural similarities to the lysostaphin-type (LAS) family of …
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Understanding and exploiting bacteriophage-host interactions for the control and detection of listeria monocytogenes
… bionanoparticles displaying the full-length endolysin of phage vB_LmoS_293 or the amidase domain of this lysin, lysed and inhibited the growth of L. monocytogenes strain 473 at 37 ºC and 22 ºC. Phages were also exploited for the capture and potential detection of L. monocytogenes (Chapter 4). …
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Prevalence and control of Clostridium difficile in patients with cystic fibrosis
… integration. However, the ФCD6356 encoded endolysin was capable of reducing viable C. difficile by 2.9 over 2 hours in vitro after being cloned and expressed in Escherichia coli.
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The Role of Lysogenic Bacteriophage in Virulence and Survival of Streptococcus Pyogenes
… genomes to genetically engineer a novel chimeric endolysin (ClyS). We demonstrated the antimicrobial lytic activity of ClyS against methicillin-resistant Staphylococcus aureus (MRSA) in both in vivo colonization and septicemia models, and were also able to show its synergistic activity with …
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Characterization of US Queso Fresco and endolysins for Queso Fresco implementation
… of addressing post processing contamination. Endolysins are a class of cell wall hydrolases derived from bacteriophages, which have been shown to be promising candidates for controlling pathogens in food systems. Endolysins derived from gram positive bacteriophages are incredibly diverse. …
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Understanding PlyP100: A precision fermentation product to improve the safety of queso fresco
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01