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Showing 1 to 20 of 26 for “"endoglucanase"”.
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Genetic Transformation of Switchgrass (Panicum Virgatum L.) with Endoglucanase Gene and Characterization of Plants with Endoglucanase Transgene
… enzymes used in the pretreatment of feedstock. Endoglucanase is the key enzyme used for breaking down cellulose before fermentation. Currently, endoglucanase is produced from engineered E. coli or yeast strains, which is still expensive for enzyme production and purification of industrial …
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Characterization of endoglucanase cela from the rumen bacterium Clostridium longisporum
… found to contain the whole celA gene encoding endoglucanase CelA, while pCM4 has a 5'-truncated gene, termed celM5', which encodes a fusion protein, CelMN', that was initiated from an ATG codon in the vector. Sequence analysis of celA revealed the presence of a type I cellulose-binding domain …
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Expression of a clostridium acetobutylicum endoglucanase and an endoxylanase in saccharomyces cerevisiae
S. cerevisiae is widely used in industrial processes, in particular ethanol production. The aim of this study was to examine the feasability of cloning lignocellulase genes into yeast. These enzymes could possibly extend the substrate range of S. cerevisiae, thereby possibly making the process of …
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The cloning and characterisation of an endoglucanase and an endoxylanase from Clostridium acetobutylicum in Escherichia coli
Clostridium acetobutylicum P262 is an endospore forming Gram-positive obligate anaerobe which has been used for the industrial production of acetone and butanol. Strains of C. acetobutylicum have been reported to exhibit some activity towards cellulosic and hemicellulosic substrates. The aim of …
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Purification and characterization of an endo-1,4-{u03B2}-D-glucanase and two exo-1,4-{u03B2}-D-glucanases from the cellulase system of Trichoderma reesei
… I (D), cellobiohydrolase II and the endoglucanase, respectively. All three enzymes have a high proportion of acidic and hydroxylated amino acids, but much less of basic amino acids. Sedimentation equilibrium studies yielded the following molecular weights for the glucanases: …
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Characterization of structure and enzymic activity of endo-l, 4-β-D-glucanases purified from Trichoderma viride
… viride enzyme preparation, Pancellase SS. These endoglucanases were distinguished by their affinity for microcrystalline cellulose; two were adsorbed onto a cellulose column at pH 5.0 (Endoglucanases III and IV) and two were not (Endoglucanases I and II). Endoglucanases II, III and IV have been …
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High-Yield Cellulosic Hydrogen Production by Cell-Free Synthetic Cascade Enzymes: Minimal Bacterial Cellulase Cocktail and Thermostable Polyphosphate Glucokinase
… glycoside hydrolase family 5 Bacillus subtilis endoglucanase, family 9 Clostridium phytofermentans processive endoglucanase, and family 48 Clostridium phytofermentans cellobiohydrolase was investigated on microcrystalline cellulose (Avicel) and regenerated amorphous cellulose (RAC). The optimal …
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Biosynthesis and identification of enzymes of the cellulase system of Trichoderma reesei
… was present, in which case aryl-β-D-glucosidase, endoglucanase and Avicelase activity were enhanced by 20, 100 and 40 percent, respectively. Under the same conditions asparagine enhanced only ary1-β-D-glucosidase activity. Some of the enzymic components of the cellulase system produced by T. …
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Cloned xylanolytic and cellulolytic enzymes from an extreme thermophile
… The carboxymethylcellulase acted as an endoglucanase, with high activity only on carboxymethylcellulose. The MUCase appeared to have two catalytic sites. The N-terminal domain acted as an exoglycobiohydrolase, with high activity on xylan, MUC and p-nitrophenyl β-D-cellobioside, while the …
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Molecular Analysis of a Cellulase/xylanase gene,celD, From the Ruminal Bacterium Ruminococcus Flavefaciens Fd-1
… data show that the cloned gene encodes both endoglucanase and endoxylanase activities. CMC and xylan zymograms of protein produced by FD1-1 and separated by non-denaturing PAGE suggest that the endoglucanase and endoxylanase activities reside on the same polypeptide. Transposon mutagenesis …
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Molecular Ecology and Evolution of Fibrobacter and Its Cellulases
… A cellulase gene, cel-3, that codes for endoglucanase 3 was chosen for comparative studies. Hybridization studies revealed that most Fibrobacter possess this gene. Phylogenetic analysis of the gene sequences demonstrated that this gene was conserved. The evolution of this gene coincides …
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Analysis of genes and enzymes involved in the degradation of cellulose and proteins by Butyrivibrio fibrisolvens H17c
… the E. coli host. It was shown that 64% of the endoglucanase activity was located in the periplasm of the E. coli host. TnphoA mutagenesis indicated the presence of a functional E. coli-like signal peptide. The nucleotide sequence of end1 was determined and the amino acid sequence (547 amino …
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Studies on the regulation of nitrogen metabolism in clostridium acetobutylicum NCP 262 and clostridium beijerinckii NCIMB 8052
… was under the control of its own promoter, the endoglucanase activity was expressed and efficiently secreted into the culture medium by C. beijerinckii NCIMB 8052. The pH and temperature optima of the enzyme activity were found to be between 5.0 and 6.0 and 45°C respectively. These parameters …
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Isothermal microwave biology : catalysis and fermentation
… results cited show that in the three systems (Endoglucanase and cellobiohydrolase with cellulose, endoglucanase and cellobiohydrolase and ~- glucosidase with cellulose, and ~-glucosidase with cellobiose) the initial rates can be increased by 201 %, 65.5% and 69% respectively. In the total …
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Avaliação em tempo real com multi detectores de reações de modificação química em xiloglucanas
… indica (XGT) e de Hymenaea courbaril (XGJ) com endoglucanase (EGII) foram analisadas em tempo real utilizando detectores de espalhamento de luz estático e viscosimetria (TDSLS-vis). Foram avaliadas três diferentes concentrações da enzima. Para ambas as amostras de xiloglucana, houve uma …
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Molecular analysis of two cellulase genes from Ruminococcus flavefaciens FD-1 and their transcriptional regulation
… residues) deduced. It showed 40% identity with endoglucanase C of Clostridium thermocellum and 27.4% identity with endoglucanase 3 of Fibrobacter succinogenes. These three enzymes are grouped into subfamily "A3". The ATG start codon of celA is preceded by a GAGG sequence, predicted to be a …
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Use of the cellulose-binding domain of a cellulase from cellulomonas fimi for affinity purification of fusion preteins
… of heterologous proteins. The CBD of endoglucanase A (CenA) from Cellulomonas fimi is an N-terminal domain comprising amino acids. CenA binds strongly to cellulose, and the CBD retains this function when separated from its cognate catalytic domain by proteolysis or genetic …
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Reducing cell wall recalcitrance in poplar through overexpression of cell wall degrading enzymes
… </p> <p>In this thesis, a hyperthermophilic endoglucanase, TnCelB, was expressed in hybrid poplar. The transgenic event with highest TnCelB expression level had reduced lignin content, cellulose crystallinity, and resultant more digestible cell wall. Post-harvest heat treatment improved …
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Operation of a Tubular Anaerobic Digested on Piggery Waste
… the digesters.<br/><br/>Extractable exocellular endoglucanase activity against carboxymethyl cellulose decreases and cell associated activity increases as slurry enters and passes along the digesters. Detectable activity is associated with particulate solids and does not disperse widely. Electron …
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