Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 2 of 2 for “"enCas12a"”.
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The IN4MER CRISPR/Cas12a Multiplex Knockout Platform and Its Applications
… platform capable of expressing four independent enCas12a guide RNAs from a single promoter. Using this platform, we designed a whole-genome library that targets not only all protein coding genes but also targets ~5,000 paralog pairs, triples and quads. This library is 30% smaller than current …
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Biases and Blind-Spots In Genome-Wide Crispr-Cas9 Knockout Screens
… 400 candidate paralog pairs using CRISPR/enCas12a dual-gene knockout screening technology and discuss the paralog synthetic lethal interactions that we have identified, which have escaped detection in monogenic CRISPR-Cas9 knockout screens. Collectively, these observations reveal …