Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 331 for “"embryonic stem cells"”.
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Human embryonic stem cells for genotoxicity testing
… mammalian cell lines and primary explanted cells commonly used in vitro to analyze the genotoxic potential of various agents, often poorly reflect human physiology. Lack of standardization also limits use of primary explanted cells. Human embryonic stem cells (hESCs) have been validated as a …
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Nuclear Transfer With Porcine Embryonic Stem Cells
… of NT embryos constructed from porcine ES cells beyond the four-cell stage. However, enucleated zygotes supported development of NT embryos beyond the four-cell stage to the compact morula stage. These results provide preliminary evidence for in vitro development of NT embryos produced from …
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Tumor-like growth of mouse embryonic stem cells
… capability of self-renewal and differentiation, embryonic stem cells (ESCs) are an invaluable tool for research in regenerative medicine. However, the main problem that hampers application of ESCs to practical stem cell therapies is that ESCs can grow as tumors when transplanted. To date, only …
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Characterization of the cardiogenesis of embryonic stem cells
… as the leading cause of mortality worldwide. Stem cell therapy, aimed to restore contractility and proper vasculature, has gained considerable attention as an attractive therapeutic option. However, proper cell differentiation, survival and integration in an infarcted zone remain elusive. This …
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Dissecting the transcriptional regulatory network of embryonic stem cells
… maintain gene expression programs in mammalian cells is fundamental to understand development and should provide the foundation for improved diagnosis and treatment of disease. Although it is not yet feasible to map the entirety of these networks in vertebrate cells, recent work in embryonic …
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Mapping the core regulatory circuitry of embryonic stem cells
Embryonic stem (ES) cells are of tremendous biological interest because they have the capacity, termed pluripotency, to generate any cell type of the adult organism. Our lab is interested in understanding the genetic circuitry that governs pluripotency. For my thesis work I have contributed to a …
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Double-Strand Break Repair Mechanisms in Human Embryonic Stem Cells
… from genotoxic and environmental stresses. Embryonic stem cells show promise for treatment of a variety of diseases as well as for providing normal human tissue to conduct scientific research. A major obstacle for their application is that genomic instability arises in stem cells after …
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Centrifugal recovery of embryonic stem cells for regenerative medicine bioprocessing
In order to realise the potential of embryonic stem (ES) cells as a regenerative medicine, it is crucial that economical, robust and scalable bioprocesses be established. Because bioprocesses irrevocably define the safety and efficacy of any biologically derived product, an understanding of the the …
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Directed differentiation of endodermal cells from mouse embryonic stem cells
Pluripotent embryonic stem cells hold a great promise as an unlimited source of tissue for treatment of chronic diseases such as Type 1 diabetes and chronic liver disease. Various attempts have been made to produce cells that can serve as precursors for pancreas and liver. By using …
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Molecular regulation of Polycomb complexes in mouse embryonic stem cells
Contains fulltext : 203027.pdf (Publisher’s version ) (Open Access)
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Establishment of the epigenetic landscape in mammalian embryonic stem cells
… phenomena has the potential to enrich both stem cell biology and therapeutics, as well as offer insight into various pathologies. While the enzymatic machinery responsible for these transitions is well known, it is their localization to specific genomic regions that controls cell fate, and …
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System to study colony-colony interactions in embryonic stem cells
… regulatory factors involved in self-renewal of embryonic stem cells (ESCs). Recent studies suggested that different autocrine factors exist in murine ESCs (mESCs) and can influence their self-renewal and proliferation, predominantly in deficiency conditions. These observations were investigated …
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Single-cell analyses of cellular reprogramming and embryonic stem cells
… paper entitled "Induced of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors." A mere two scientists reprogrammed somatic cells to an embryonic stem-cell like state (termed induced pluripotent stem cells, iPSCs) by simply overexpressing four …
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Roles for chromatin regulators during differentiation of embryonic stem cells
… pattern controlling its cellular state. As cells differentiate into specialized cell types, changes in the gene expression program occur with associated changes in chromatin. An understanding of the roles for chromatin regulators in the control of gene expression programs during …
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Stimulations and modulations of chondrogenic differentiation of human embryonic stem cells
By using human embryonic stem cells (hESCs) as the model system, this study aims to understand the full span process of chondrogenesis, and how this process could be regulated by growth factors BMP2 and/or TGFI?1 in a controlled context both in vitro and in vivo. By culturing hESCs in 2-D EB …
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Fibronectin peptides and murine embryonic stem cells : an in vitro study
Murine embryonic stem cells are cells that are obtained from the inner cell mass of the embryo blastocyst at day 3.5. These cells have the novel capability of differentiating into almost any cell lineage in the mammal and this characteristic has resulted in them being referred to as pluripotent. …
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Microfluidic Devices for the Investigation of Pluripotency in Embryonic Stem Cells
… designed to facilitate research into mouse embryonic stem cells (ESCs). ESCs are a well-studied cell, largely due to their pluripotent nature, meaning they are able to differentiate into all cell types of the body and may self-renew indefinitely in appropriate culture conditions. ESCs, along …
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Modelling metabolic landscapes in human naive and primed embryonic stem cells
… this transition can be modelled with pluripotent cells in culture which exist in a naive (more pluripotent) and a primed (epiblast like) state and show the same differences in metabolic regulation. These two cell states also show major differences in their epigenetic landscape including in DNA …
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