Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"embryonic stem cell (ESC)"”.
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Molecular mediators of cardiac-specific enhancer activation
… of how these binding events regulate cell specification. To identify potential key regulatory TFs, we used the Assay for Transposase-Accessible Chromatin (ATAC-seq) to map changes in chromatin accessibility and integrated these data with maps of histone modification patterns and gene …
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The NuRD complex regulates chromatin dynamics driving exit from naïve pluripotency
… exit from naive pluripotency both in mammalian embryonic stem cell (ESC) culture and in vivo. However, the specific mechanisms of chromatin modulation by NuRD in this process have not been fully characterised. This study used rapid, auxin-inducible removal of NuRD components in mouse ESCs to …
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Investigating the role of Neuronatin in neural development
… is converted into neural tissue. However, the cellular and molecular mechanisms that govern this process are still not completely understood. For instance, in Xenopus, calcium signalling is known to play a pivotal role in neural induction but whether a similar mechanism occurs in mammals is …
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Cell-to-cell variability and culture conditions during self-renewal reversibly affect subsequent differentiation of mouse embryonic stem cells
Cell-to-cell variability in clonal populations is reflected in a distribution of mRNA and protein levels among individual cells, including those of key transcription factors governing embryonic stem cell (ESC) pluripotency and differentiation. This may be a source of heterogeneity resulting in …
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Developing Serum-Free Media Via Bioprocessing For Cultivated Seafood Products
… food source due to its widespread consumption, excellent nutrient profile, and low feed conversion ratio, rendering its sustainable production quintessential. Cellular agriculture or cultured meat can increase seafood production; however, the conventional use of Fetal Bovine Serum (FBS) in culture …
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Signaling pathways regulating self-renewal, differentiation, and multipotency of CD133+ umbilical cord blood stem cells
… the number of immature umbilical cord blood stem cells (UCBSCs) for hematopoietic transplantation. Towards this goal, our lab adapted a culture technique to grow immature CD133+ UCBSCs (CD133+ cells). Although CD133+ cells proliferate rapidly in culture, a minority self-renew and remain …
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Developing High Purity Embryonic Stem Cell-Derived V2a Interneurons for In Vitro Investigation and Transplantation Following Spinal Cord Injury
… lesion to activate pre-existing motor pathways. Cell replacement strategies and growth factor delivery platforms often attempt to enhance these natural mechanisms of regeneration. The work of this thesis sought to overcome the major obstacle to manipulating specific interneurons for therapy: an …
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The immunogenicity of embryonic stem cell-derived midbrain dopaminergic progenitors and its implications as a therapy to treat patients with Parkinson’s Disease
… alleviate the symptoms, they do not prevent the cell death. As such, stem cell-based DA cell replacement therapies provide a promising treatment option, however little is understood about their immunogenicity. This PhD project investigates the immunogenicity of these cells and their implications …
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The Dual Role of Notch Signaling During Motor Neuron Differentiation
… into motor columns and pools based on cell body settling position, general axonal trajectories, and the individual muscles they innervate. Elegant studies have demonstrated that motor neuron columnar and pool diversity, along the rostral-caudal axis of spinal cord, is programmed by …
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Mechanotransduction Modulates the Effect of Mechanical Forces (Fluid Shear Stress and Cyclic Strain) on Embryonic Stem Cell Differentiation Toward Vascular Endothelial Cells
<p>The isolation of human ES cells (hES) in 1998 remarkably elevated the interest in the cell therapy capability of ES cells and moved this concept one step closer to reality. Embryonic stem cells with their potential for both self-renewal and directed differentiation have received increasing …
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Eomes Directs the Formation of Spatially and Functionally Diverse Extra-Embryonic Mesoderm Derived Tissues
During mouse embryonic development, the first mesodermal cells to originate at the posterior primitive streak will give rise to the extra-embryonic mesoderm (ExEM). These cells will contribute to key tissues supporting the development of the embryo, including the amnion, chorion, yolk-sac, and …
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Cyclic loading of fibronectin coated beads induces differentiation in mouse embryonic stem cells
The role of soluble factors in determining embryonic stem cell fate has been well studied. A growing body of research indicates mechanical cues are equally important to chemical signals in eliciting cellular response, especially during the early developmental stages of embryos. Here, we seek to …
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Prediction method of condition-specific protein-protein interactions using gene expression profiles
… 및 종양세포에서 조건 특이적인 PPIs 데이터를 구축하고, 이 중 배아 줄기세포(ESC, embryonic stem cell)의 GEP 를 이용한 ESC-특이적 PPIs 예측정도를 여러 정규화 방법 (Normalization methods) 및 유전자 공동 발현패턴 (Gene co-expression pattern) 측정법을 통해 종합적으로 비교분석하였다. 이를 바탕으로 GEP 데이터를 이용한 유전체 수준의 조건 특이적인 PPIs를 예측하는데 있어 적합한 방법론을 제안하는데 그 연구목적이 있다. 조건 특이적인 PPIs …