Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 136 for “"embryonic stem cell"”.
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H4K16 acetylation during embryonic stem cell differentiation
… component of the doseage compensation system. It is also unique amongst histone modifications in that it has been directly associated with chromatin decompaction. H4K16ac has been linked to development through its Histone Acetyltransferase, MOF. Deletion of MOF in mice leads to mass …
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Molecular control of embryonic stem cell identity
Embryonic Stem (ES) cells are the in vitro derivatives of the inner cell mass of a developing embryo, and exhibit the property of pluripotency, which is the ability of a cell to give rise to all cell lineages of an organism. Therefore, these cells hold great promise in the treatment of several …
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Understanding the Dynamics of Embryonic Stem Cell Differentiation
The two defining features of mouse embryonic stem (ES) cells are self-renewal and naive pluripotency, the ability to give rise to all cell lineages in the adult body. In addition to being a unique and interesting cell type, pluripotent ES cells have demonstrated their potential for continued …
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Identification of Undifferentiated Human Embryonic Stem Cell-Specific Genes
Embryonic stem cells are derived from mammalian blastocysts and self-renewal and maintain pluripotency, an ability to differentiate into all types of somatic and germ cells. Recent reports showed that several genes such as Pou5F1 (Oct4, Oct3/4), Sox2, Nanog, LIN28, KLF4, Myc(c-Myc) maintained a …
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Regulated Histone H3 Proteolysis During Mouse Embryonic Stem Cell Differentiation
… chromatin often lead to dynamic changes in many cellular processes, including development and differentiation, by affecting the accessibility of the genomic information stored in the DNA. The cell uses many different mechanisms to regulate chromatin in order to establish, maintain, and propagate …
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The regulation of mouse embryonic stem cell differentiation by Nrf2
Embryonic stem (ES) cell maintenance and differentiation are dynamic processes controlled by various intrinsic and extrinsic factors. Identifying these factors will enhance the understanding about developmental process and improve the application of stem cells in clinic. Previous studies highlight …
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Elucidating the Role of Plakoglobin in Embryonic Stem Cell Culture
Pluripotency – the ability of cells to differentiate into all three germ layers – is of great interest, particularly in the fields of developmental biology and medicine. The in vivo continuum can be separated into three in vitro states that are also found in the embryo: naïve, formative, and primed …
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Transcription factor antagonism regulates heterogeneity in embryonic stem cell states
Gene expression heterogeneity underlies cell states and contributes to developmental robustness. While heterogeneity can arise from stochastic transcriptional processes, the extent to which it is regulated is unclear. Here we characterize the regulatory program underlying heterogeneity in murine …
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The Role of Snail Homolog 1 in Embryonic Stem Cell Differentiation
During gastrulation, epiblast cells undergo an epithelial-to-mesenchymal transition: EMT) as they ingress through the primitive streak and form mesoderm. To better understand the molecular pathways of EMT during this developmental transition, we developed a model system utilizing mouse embryonic …
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Studying the direct effects of forces on embryonic stem cell behaviour
Cells experience different mechanical cues from their local environment, including shear flow, forces applied by neighbouring cells, and substrate stiffness. These external signals influence cell behaviour, also in embryonic stem (ES) cells, where they could potentially affect pluripotency or …
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Effects of Trimethylamine N-Oxide on Mouse Embryonic Stem Cell Properties
… oxygen species (ROS) generation in numerous cell types, resulting in cell dysfunction. However, whether TMAO will impact stem cell properties remains unknown. This project aims to explore the potential impact of TMAO on mouse embryonic stem cells (mESCs), which serve as an in vitro model of …
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The Functions of Setd5 and Mir-221 In Embryonic Stem Cell Differentiation
<p>Embryonic stem cells (ESCs) are a widely used model system to study cellular differentiation because of their pluripotent characteristics, and ESC differentiation is an epigenetic process. In an effort to identify a new epigenetic factor that is required for ESC differentiation, the function of …
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A chemical biology study of human embryonic stem cell pluripotency and differentiation
Human embryonic stem cell (hESC) research provides promising prospects for the future of regenerative medicine. To fully realize its potentials, we not only need to further our understandings toward the molecular mechanisms governing pluripotency and differentiation, but also to find new tools to …
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The cell-secreted microenvironment : shaping embryonic stem cell self-renewal and differentiation
… is to obtain an in depth understanding of how embryonic stem cell-secreted signals contribute to their identity. We analyze the contribution of broad and specific signals present in the cell-secreted microenvironment using techniques that can easily be applied to studies of other cell types and …
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The role of Polycomb-mediated epigenetic regulation in embryonic stem cell differentiation
During mammalian development, a single founding cell must produce all of the different types of cells in the adult organism. What are the regulatory mechanisms required to coordinate the necessary gene expression networks for this process? Polycomb group (PcG) proteins are epigenetic regulators …
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P120-Catenin Regulates Rest and Corest, and Modulates Mouse Embryonic Stem Cell Differentiation
… studied to determine their contributions to stem cell biology, but less is known about p120-catenin in the nuclear compartment. P120 is developmentally required as a consequence of its biochemical and functional interactions with cadherins, small-GTPases and transcriptional regulators. We …
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Encapsulation as a 3D model for human embryonic stem cell propagation and differentiation
… disease caused by the destruction of the beta cells of the pancreas. Current treatments for are whole pancreas or islet transplantation, which is limited by scarcity of cadaveric pancreata. Therefore, developing a cell replacement therapy with renewable sources of surrogate beta cells is …
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Development of a bioreactor imaging system for characterizing embryonic stem cell-derived cardiomyocytes
… tissues such as the heart is limited, human embryonic stem cells (hESC) have emerged as a source for potential cardiac therapies. However, despite the use of a variety of biochemical differentiation protocols, current yields of hESC-derived cardiomyocytes (CM) have been low. In the case of …
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The Role of Nitric Oxide and Peroxynitrite in Human Embryonic Stem Cell Differentiation
Human embryonic stem cells (hESCs) have the potential to grow into almost all types of cells in the human body. This special property makes hESC invaluable for the restoration of tissue function after damage from different diseases like Parkinson's disease, Alzheimer’s disease, stroke, and …
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