Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"embryoid body"”.
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Effects of Diethylstilbestrol on Murine Early Embryonic Stem Cell Differentiation Using an Embryoid Body Culture System
… an OP9 co-culture system, and formation of embryoid bodies (EBs) in a liquid medium and hanging drop system. The OP9 co-culture system did not appear to give rise to well differentiated lymphoid lineage cells during 12 days of differentiation. The hanging drop EB culture system, previously …
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Contribution of Lsh to DNA methylation reprogramming in embryonic stem cell, epiblast stem cell and embryoid body model systems
… (‘serum’ mESCs, epiblast stem cells and embryoid bodies). Implementation of this model system suggests that Lsh does not contribute to DNA methylation establishment in a pluripotent context, but rather is important for facilitating de novo DNA methylation during differentiation to culture …
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Directed differentiation of endodermal cells from mouse embryonic stem cells
… retinoic acid and basic-FGF, in the absence of embryoid body formation, mouse embryonic stem cells were differentiated at different culture periods. Four protocols of varying lengths of culture and reagents and their cells were analyzed by quantitative PCR, immunohistochemistry and static …
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Gcn5 Impacts Fgf Signaling At Multiple Levels and Activates C-Myc Target Genes During Early Differentiation of Embryoid Bodies
… project, we use a murine ESC aggregation assay (embryoid body formation, EB) to model the early development stages that proceed gastrulation, and report that GCN5, a histone acetyltransferase (HAT) essential for embryonic development, is required for proper expression of multiple genes encoding …
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Characterization of the Effects of bFGF and GSK3β Inhibitors on Embryoid Bodies Derived from Human Pluripotent Stem Cells
<p>Embryoid body (EB) formation is a common first step in many human pluripotent stem cell (hPSC) differentiation protocols. Previous work suggests that EBs are sensitive to growth factor withdrawal if they are derived from hPSCs maintained in feeder independent media such as mTeSR1. To promote …
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Neural differentiation of pluripotent stem cells and applications to micropatterned agarose hydrogel for nerve guidance
… for nerve guidance. A protocol utilizing embryoid body (EB) formation, under both high and low glucose conditions, was devised for RA-induced neural lineage differentiation and RA/shh-induced oligodendrocyte differentiation. EBs exposed to lower glucose concentrations with agonists …
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Extracellular matrix based substrates for propagation of human pluripotent stem cells
… SSEA4) c) in vitro differentiation potential by embryoid body formation d) Real time RT-PCR analysis for pluripotent and germ-layer specific markers and e) karyotype analysis for chromosome number. Compositional characterization of the ECM substrates using proteomic analysis identified some of …
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Gene expression studies of pregastrulation development: the basement membrane is essential for cell differentiation
… This process is faithfully mimicked in vitro by embryoid body (EB) cultures. Fibroblast growth factor (FGF) signaling is crucial when the step-like process of EB development is initiated with the formation of an endoderm. A subendodermal BM is formed, in which the globular domains LG4-5 of the …
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Heparan sulfate glycosaminoglycan regulation of vasculogenesis
… HSGAG in vasculogenesis. An embryonic stem cell embryoid body model was used to establish the necessity of sulfated HSGAG for endothelial differentiation. We identified that the chemical composition of HSGAG sulfation patterns change with differentiation. Perturbation of HSGAG structure by …
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Investigating the Role of the mRNA Capping Enzymes in Mouse Embryonic Stem Cells and Differentiation
… was found to be upregulated during embryoid body formation, LIF withdrawal and in vitro neural differentiation. The function of CMTR1 in mESCs and differentiation was explored by producing a Cmtr1<sup>∆S30-D31 </sup>cell line by CRISPR-Cas9, although it was not possible to obtain …
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Investigation of genetic PIK3CA activation in genome-edited human pluripotent stem cells
… NODAL, resulting in self-sustained “stemness” in embryoid body and teratoma assays. In comparison, heterozygous mutants behaved similar to wild-type controls under all differentiation paradigms. Furthermore, evidence was obtained that strong activation of PI3K signalling is fully compatible with …
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Investigation of the mechanisms underlying the effects of hyperglycaemia on cardiac structural and electrical remodelling
… into pulsatile, cardiac-like cells via embryoid body (EB) formation and cultured under baseline- or high glucose conditions. A Ca2+ -sensitive fluorescent dye Fluo-4 was used to measure calcium transients and a voltage-sensitive dye di-4-ANEPPS was used to record action potentials. …
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Hyperosmotic Stress Enzyme Signaling Modulates Oct4, Nanog, And Rex1 Expression And Induces Prioritized Differentiation Of Murine Embryonic Stem Cells
… were continued in the peri-implantation model, embryoid bodies</p> <p>(EBs), in which differentiation is allowed rather than actively suppressed. Unstressed</p> <p>EB culture recapitulated the lineage inductions of in vivo embryos. EBs were only able</p> <p>to be cultured in the presence of low …
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Induced-Pluripotent Stem-Derived Neuronal Progenitor Cells As A Novel Treatment For Neurodegenerative Diseases
… capabilities will be evaluated by teratoma and embryoid body formation assays.</p> <p>Specific aim 2: To differentiate IPS cells to a neuronal lineage. IPS cells will be differentiated to a NP stage by the use of defined media culture conditions. NP cells will be characterized by their …
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Engineering the micro-environment to control the fate of mammalian cells
… the aligned and the non-aligned samples. Since, embryoid body formation is the precursor to the differentiation of mouse embryonic stem cells, both the viability and alignment of embryoid bodies was verified in the hydrogels as well. Also, some preliminary results are presented that show the …
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Extracellular matrix stiffness and geometry: The impact on static and dynamic cellular systems in vitro and the treatment of tissue ischemia
… and endothelial differentiation within embryoid bodies through the control of three-dimensionality on collagen-polyacrylamide gels with tunable stiffness (Chapter 2). Next, the function of airway smooth muscle cells is investigated on substrates with physiological and pathological …
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Development of fish embryonic stem cell lines as tools for basic and applied studies of interest in aquaculture
… their growth and potential in terms of embryoid body formation, differentiation and transfection capacity. In addition, we have analysed the evolution of cell proliferation under different photoperiods, revealing significant daily oscillations in the proliferative activity of these …