Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"embryoid"”.
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Effects of Diethylstilbestrol on Murine Early Embryonic Stem Cell Differentiation Using an Embryoid Body Culture System
… an OP9 co-culture system, and formation of embryoid bodies (EBs) in a liquid medium and hanging drop system. The OP9 co-culture system did not appear to give rise to well differentiated lymphoid lineage cells during 12 days of differentiation. The hanging drop EB culture system, previously …
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Characterization of the Effects of bFGF and GSK3β Inhibitors on Embryoid Bodies Derived from Human Pluripotent Stem Cells
<p>Embryoid body (EB) formation is a common first step in many human pluripotent stem cell (hPSC) differentiation protocols. Previous work suggests that EBs are sensitive to growth factor withdrawal if they are derived from hPSCs maintained in feeder independent media such as mTeSR1. To promote …
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Gcn5 Impacts Fgf Signaling At Multiple Levels and Activates C-Myc Target Genes During Early Differentiation of Embryoid Bodies
… project, we use a murine ESC aggregation assay (embryoid body formation, EB) to model the early development stages that proceed gastrulation, and report that GCN5, a histone acetyltransferase (HAT) essential for embryonic development, is required for proper expression of multiple genes encoding …
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Contribution of Lsh to DNA methylation reprogramming in embryonic stem cell, epiblast stem cell and embryoid body model systems
… (‘serum’ mESCs, epiblast stem cells and embryoid bodies). Implementation of this model system suggests that Lsh does not contribute to DNA methylation establishment in a pluripotent context, but rather is important for facilitating de novo DNA methylation during differentiation to culture …
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In Vitro Models of Cellular Dedifferentiation for Regenerative Medicine
… cMyc, Klf4, and Nanog), and the ability to form embryoid bodies that expressed markers of all three germ layers. Additionally, we were able to create secondary porcine iPSC lines by exposing cellular outgrowths from embryoid bodies to doxycycline to initiate more efficient production of porcine …
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First evidence of skelemin, a myosin-associated protein, in smooth muscle and its involvement in cell adhesion, and, The role of the cell cycle in cell type choice during mammalian development
… of skelemin protein in developing embryoid bodies. Skelemin underexpression was seen to cause loss of cell-cell as well as cell-substrate adhesion. Time lapse video microscopy was used to analyze the role of the cell cycle in cell type choice during mammalian development. Discussed …
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Utilising embryonic and extra-embryonic stem cells to model early mammalian embryogenesis in vitro
… into three-dimensional (3D) aggregates, called embryoid bodies, can become patterned and express genes associated with early tissue differentiation. However, embryoid bodies cannot recapitulate embryonic architecture and therefore may not accurately reflect what happens in the embryo. In this …
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Mitochondrial DNA replication in pre-implantation embryonic development
… decreased as murine ESCs differentiated into embryoid bodies, although expression of mtDNA replication factors did not increase until the stage equivalent to organogenesis. Cross-species NT embryos in which the donor cell-derived mtDNA was replicated produced decreased developmental outcomes …
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Hyperosmotic Stress Enzyme Signaling Modulates Oct4, Nanog, And Rex1 Expression And Induces Prioritized Differentiation Of Murine Embryonic Stem Cells
… were continued in the peri-implantation model, embryoid bodies</p> <p>(EBs), in which differentiation is allowed rather than actively suppressed. Unstressed</p> <p>EB culture recapitulated the lineage inductions of in vivo embryos. EBs were only able</p> <p>to be cultured in the presence of low …
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THE ALTERNATIVE SPLICING OF NF-YA REGULATES MOUSE EMBRYONIC STEM CELLS DIFFERENTIATION
… and stemness maintenance. By contrast, during Embryoid Bodies (EB) formation process, NF-YAl prevails, suggesting an implication of this specific isoform in the differentiation of mESCs. Our data strengthen the stem-related function of NF-YAs and propose a novel role of NF-YAl in mesoderm …
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Directed differentiation of endodermal cells from mouse embryonic stem cells
… retinoic acid and basic-FGF, in the absence of embryoid body formation, mouse embryonic stem cells were differentiated at different culture periods. Four protocols of varying lengths of culture and reagents and their cells were analyzed by quantitative PCR, immunohistochemistry and static …
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Extracellular matrix stiffness and geometry: The impact on static and dynamic cellular systems in vitro and the treatment of tissue ischemia
… and endothelial differentiation within embryoid bodies through the control of three-dimensionality on collagen-polyacrylamide gels with tunable stiffness (Chapter 2). Next, the function of airway smooth muscle cells is investigated on substrates with physiological and pathological …
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In Vitro Regeneration and Genetic Transformation of Sesbania Drummondii: A Medicinally and Environmentally Important Plant
… its mass proliferation of calli having numerous embryoid-like structures. Indole-3-butyric acid (0.24 - 2.46 |aM) was found suitable for root induction. In vitro regenerated plants were acclimatized in greenhouse conditions. This report is a first report on studies of in vitro regeneration of S. …
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Control of cell fate decisions of embryonic stem cells by mechanical forces
… on the soft substrates formed more efficient embryoid bodies and teratomas than those on rigid substrates. Collectively, these results strongly suggest that mechanics is indispensable in physiological functions of embryonic stem cells.
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Downregulation of reproductive homeobox gene 6 (RHOX6) interferes with male germ cell differentiation
… PGCs when ESCs are cultured as aggregates called embryoid bodies (EBs). By using a mouse ESC line that expresses the enhanced green fluorescent protein (EGFP) under the promoter of Oct3/4, a central regulator of cellular pluripotency, the presence of PGCs within EBs was monitored in real time by …
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The Generation of a Candidate Axial Precursor in Three Dimensional Aggregates of Mouse Embryonic Stem Cells
… through large cellular aggregates known as embryoid bodies. These structures do not, however, resemble the axial organisation of the embryo and they develop in a disordered manner. This thesis presents a novel culture system of small, three-dimensional aggregates of ESCs (gastruloids) that …
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Engineering the micro-environment to control the fate of mammalian cells
… the aligned and the non-aligned samples. Since, embryoid body formation is the precursor to the differentiation of mouse embryonic stem cells, both the viability and alignment of embryoid bodies was verified in the hydrogels as well. Also, some preliminary results are presented that show the …
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Neural differentiation of pluripotent stem cells and applications to micropatterned agarose hydrogel for nerve guidance
… for nerve guidance. A protocol utilizing embryoid body (EB) formation, under both high and low glucose conditions, was devised for RA-induced neural lineage differentiation and RA/shh-induced oligodendrocyte differentiation. EBs exposed to lower glucose concentrations with agonists …
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Molecular analysis of transcriptional properties of Pbx1 and E2a-Pbx1
… cells up to the day 9 after reattachment of the embryoid bodies (EB) to the adhesive media. Parallel with observation of intensified neural and glial gene
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