Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 32 for “"embryo culture"”.
-
In Vitro Maturation, Fertilization and Embryo Culture of Porcine Gametes in a Microfluidic Environment
… compare the results to the traditional microdrop culture system. Additionally, the initial experiments of integrating the steps of the IVP process within a single microchannel were investigated in order to determine some baseline parameters when using this novel IVP system.
-
Investigating a novel in vitro embryo culture system – The Walking Egg Affordable Assisted Reproductive Technology
… such initiative is the development of a low-cost embryo culture method by The Walking Egg foundation. This method utilizes a standard chemical reaction and simple equipment to equilibrate culture media pH and to regulate temperature; both aspects were investigated in this study. An exploration …
-
Optimizing embryo culture conditions and spent culture media analysis as predictors of embryo quality and pregnancy
… aim of this thesis is first, to evaluate various culture conditions to improve embryo development, and secondly, to analyze spent culture media for any biomarkers that may be predictive of embryo health. Single-step and sequential culture media were compared in both Planer and EmbryoScope™ …
-
Supplementing Bovine Embryo Culture Media to Improve the Production and Quality of In Vitro Produced Bovine Embryos
… factor (LIF) in preimplantation bovine embryos. Neither cytokine affected the total percentage of embryos which developed to the blastocyst stage in vitro. However, supplementation of IL6 increased blastocyst inner cell mass (ICM) cell number without affecting trophectoderm (TE) cell …
-
A study of the teratogenicity of diphenylhydantoin and phenobarbitone in the experimental mouse
… in mice both in vivo and in an in vitro whole embryo culture system, to investigate possible mechanisms of teratogenicity and to examine whether the methods used in this study, may form a basis for developing systems of more extensive drug teratogenicity screening.
-
Developing embryo technologies for the eland antelope (Taurotragus oryx)
… effects of components/attributes of protein-free embryo culture media on the in vitro development of in vitro-derived bovine embryos were evaluated. A 2 x 2 factorial study comparing effects of groups of amino acids (20aa or 11aa) in two base media (modified KSOM or BM-3) demonstrated that amino …
-
Post-Transfer Outcomes in Cultured Bovine Embryos Supplemented with Epidermal Growth Factor, Fibroblast Growth Factor 2, and Insulin-Like Growth Factor 1
… Use of in vitro fertilized (IVF) bovine embryos has become increasingly popular to help alleviate several of these reproductive issues and provide a means to enhance genetic gain for production traits. An uterine paracrine factor cocktail containing epidermal growth factor (EGF), …
-
Establishment of a phenotypical model of adverse outcomes associated with assisted reproductive technologies
… that is observed in ruminants as a result of embryo culture. This syndrome is known as large offspring syndrome (LOS). The genomic region/s associated with LOS have not yet been determined. We hypothesized that BWS and LOS are epigenetically similar. The aim of this research was to ascertain …
-
Nuclear Transfer With Porcine Embryonic Stem Cells
Results from embryo culture experiments indicated: (1) bovine serum albumin (BSA) supplementation improved developmental rates of four- and eight-cell Meishan embryos to the blastocyst stage, (2) fetal bovine serum supplementation improved hatching rates of later stage Meishan embryos, (3) fatty …
-
Effects of Polydatin on In Vitro Bovine Embryo Developmental Competence, Metabolism, and Cryopreservation
<p>Bovine <em>in vitro</em> produced embryos suffer from poor developmental competence and altered metabolism which hinders their cryotolerance. Overall, the goals of this thesis were to improve oocyte and embryo culture with the antioxidant polydatin and to optimize slow freezing procedures. This …
-
Profiling L-serine Transport Throughout Growth and Meiotic Maturation in Mouse Oocytes
… success rates of approximately 40%, oocyte and embryo culture conditions in vitro can be improved by first understanding the finer details of oocyte function. As such, there is a need to better understand the mechanisms through which oocytes can acquire certain nutrients. This thesis focuses on …
-
Altered microrna expression profiles in bovine fetuses with an assisted reproduction-induced congenital overgrowth syndrome
… 1 will also introduce LOS including studies on embryo culture in various species, gene expression studies, and previous studies from our laboratory. In addition, chapter 1 will introduce miRNAs including its biogenesis and function. Chapter 2, which is the research chapter, will show the data of …
-
Exploring the effects of insulin and glucose on the development of in vitro produced domestic cat embryos
… the effects of glucose and insulin on feline embryonic development using in vitro fertilization and embryo culture. The objective of this study was to determine if glucose concentrations greater than ≥ 6 mM with or without varying concentrations of insulin would affect cleavage or blastocyst …
-
Microfluidic tools for metabolomics
A primary challenge in embryology is to understand the factors that govern the development of preimplantation (PI) embryos and how these factors relate to embryo viability in the field of in vitro fertilization (IVF). This is particularly important as clinical policy moving towards single embryo …
-
Effects of Dietary Conjugated Linoleic Acid Supplementation on Bovine Oocyte Lipid Metabolism, Lipid Composition and Embryo Cryotolerance
Variation in cryotolerance exists between embryos from different animal breed, species and management conditions. Reduced tolerance to chilling and cryotolerance of oocytes and embryos has been associated with greater cytoplasmic lipids (Kim et al., 2001; Seidel, 2006). Previous studies in the cow …
-
Interleukin-6 and its Contribution to Embryogenesis in Cattle
In vitro systems like those used for in vitro embryo production are invaluable for our understanding of embryogenesis and the processes that regulate it. However, extensive research has also highlighted that in vitro produced embryos negatively differ from their in vivo counterparts in various …
-
Lipid Quantification and Cryopreservation of In Vitro Produced Jersey Cattle Embryos
… of in vivo derived Jersey bovine embryos have resulted in a 10% lower pregnancy rate compared to other dairy breeds. Poor embryo survival after cryopreservation has been partially attributed to the high lipid content of Jersey embryos. In vitro-produced (IVP) bovine embryos have …
-
An in vitro model of human yolk sac and epiblast crosstalk
Embryogenesis entails complex signalling interactions between embryonic and extraembryonic lineages. Improved embryo culture techniques have recently allowed human embryos to be cultured in vitro past the point at which they would implant in vivo, until the internationally accepted culture limit of …
-
Supplementing IL6, IL11, and LIF to Improve Cultured Bovine Oocyte Competency
Bovine embryos produced in vitro consistently display decreased quality in terms of their potential to reach the blastocyst stage as well as post-transfer survival. Media formulations, oocyte quality, and inferior expression of needed transcripts are all causes of this reduced developmental …
Page 1 of 2