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Showing 1 to 20 of 242 for “"elution"”.
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Elution of Metronidazole and Gentamicin from Polymethylmethacrylate Beads
… metronidazole were evaluated. Antibiotic elution patterns were similar for all groups. Day-1 elution for groups containing either metronidazole (3 concentrations) or gentamicin represented a mean 63% to 66% and 79% respectively of the 21-day total elution. Approximately 50% of the day-1 …
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Effect of column temperature on elution order in gas chromatography
… in gas chromatography (GC). Reversal of peak elution order at different temperatures is observed when selectivity is sufficiently changed by changing the temperature. A study of homologous functional groups and the elution order of a test mixture was made at several temperatures, by keeping …
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Elution of Antibiotics from a Novel Cross-linked Dextran Gel: In vivo Quantification
Amikacin-, vancomycin- or amikacin/clindamycin-impregnated gel was placed subcutaneously on either side of horses' necks a total of 6 times each. Interstitial fluid was collected at 0, 4, 8, 12 and 24 hours, and days 2 through 10, via capillary ultrafiltration probes placed within the incision …
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Evaluation of the in vitro elution characteristics of carboplatin-impregnated calcium sulfate beads
Pilot Study: Objective: To characterize the elution of platinum from carboplatin-impregnated calcium sulfate hemihydrate (CSH) beads in vitro. Sample: Sixty carboplatin-impregnated CSH beads and 9 CSH beads without added carboplatin (controls) Procedures: Carboplatin-impregnated CSH beads (each …
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Interfacing of an LSI-11 micro processor with the spectra-physics 3500B gradient elution liquid chromatograph
… and a Spectra-Physics model 3500B gradient elution liquid chromatography were interfaced for the purpose of automating the chromatograph, incorporating it into the laboratory data network system and collecting data for Michael Starlings' project. The automation involved the construction of …
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A study to investigate the persistence of polydimethylsiloxane and to determine the detection of polydimethylsiloxane in a body fluid matrix following Cellmark’s Sperm Elution©
… the swab head extracts using u-ATR after Sperm Elution. However, it is currently unknown whether the addition of multiple body fluids, in addition to semen, affects whether PDMS is able to persist on the swab head. Eight different body fluid combinations of semen, blood, saliva, vaginal material …
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Direct analysis of dried blood spot samples
… to mass spectrometers (MS), resulted in direct elution (direct extraction of DBS via a confined solvent, producing a liquid extract) being selected as the most suitable technique to develop for this application. Direct elution technology was then developed into fully automated techniques with …
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Endotoxin removal from protein solutions by immobilized metal ion affinity chromatography
… determined. Fourth, the endotoxin or protein elution process was developed. Different elution schemes were investigated including buffer pH, ionic strength, and elution competing agent. The change of solution ionic strength and introduction of NH_4Cl in elution buffer are not efficient to …
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Measuring Kinetic Performance of Monolithic and Fused-Core Columns Using Packed-Capillary Liquid-, Ultrahigh-Performance- and Supercritical Fluid Chromatography
… can be used for both isocratic and gradient elution and is useful in the comparison of different types of chromatographies (HPLC, GC, CEC, SFC). The KPM was used to compare the performance of packed-capillary (0.2 and 0.3 mm) and microbore (2.0 and 2.1 mm) superficially porous and monolithic …
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Advances in DNA Affinity Chromatography
… chromatography such as DNA complexity heparin elution, the Bi-column method and the oligonucluotide trapping method were studied. The complexity (length) of a DNA sequence attached to an affinity chromatography column affects column retention, and the purity of transcription factors obtained. …
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Treatment of plating plant wash waters by ion exchange
… loaded metals by complexation techniques during elution has been demonstrated. Good agreement between predicted and actual elution conditions for the particular separations have been achieved. The loading of free and metal-complexed cyanide and chromate on a strong base resin has been shown to be …
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Supercritical fluid chromatography of nitrogen-containing compounds on packed columns
… aromatic amines and azaarenes shows that the elution order not only depends on basicity and steric hindrance, but also on the solubility of the solute in CO₂. New cross-linked cyanopropyl and phenyl bonded phases are studied as stationary phases for packed column SFC, as well as for separation …
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The isolation and characterisation of MHC-presented peptides from CML-derived cell-lines, with a focus on post-translational modification
… to the neoplastic state. Mild acid cell-surface elution is a rapid and effective method for MHC class-I peptide capture, though complicated by contamination with non-MHC peptides and poor downstream compatibility, especially with IMAC, a popular method for phosphopeptide enrichment. As an …
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Improving DNA evidence collection via quantitative analysis: a systems approach
… surface area that may be processed, possible co-elution of PCR inhibitors, and non-optimized elution of cells from the substrate into solution. Therefore, a technique designed to optimize biological collection from items of interest, particularly large items, is necessary and not currently …
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Investigating methods for the speed-up of intra-lanthanide chromatographic systems
… separation. The development of a chromatography elution gradient generally requires many experiments due to the large number of variables that contribute to column performance. The development time for new gradients can be months to years. The first portion of this project aims to drive down …
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Entwicklung einer Technologie zur langzeitstabilen Biologischen Reinigung schwermetallbelasteter Bergbauwässer
… The organic compounds are either supplied by elution or by hydrolysis of polymeric compounds which was named active elution. The concept was realised as a two-phase process with (active) elution in the first phase (R1) and sulphate reduction and metal elimination in the second phase (R2). With …
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A MODEL SYSTEM FOR THE CONCENTRATION OF REOVIRUS FROM AQUEOUS ENVIRONMENTS
<p>A filter adsorption-elution method for the routine isolation of reovirus from model aqueous systems was developed. Reovirus was found to be stable within a pH range of 3 to 10, with a minimum loss of viability. A slight hydrogen ion excess minimized virus aggregation phenomena and promoted …
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Characterization of Everolimus In Biodegradable PLAF/PLEOF Nanoparticle
… and delivery of Everolimus from DES. Elution of Everolimus was measured from Poly (lactide-fumarate)(PLAF) and Poly (lactide-co-ethylene oxide fumarate))(PLEOF) nanoparticle. Synthesis of the 90%PLAF/10%PLEOF NPs and characterization with respect to particle size distribution, …
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Purification of the major envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions
… protein, N, was a major impurity in CEX elution fractions. As a second chromatographic step, hydrophobic interaction chromatography (HIC) further purified GP5 by means of a two-stage elution scheme. Pure GP5 was eluted from the HIC resin in the second HIC elution stage by Triton X-100 …
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An investigation of the combined use of cation exchange enrichment and spectrochemical analysis for the determination of trace constituents in common silicate rocks and chondrites
… exchange chromatography with hydrochloric acid elution was employed to resolve the complex mixtures obtained from rocks and meteorites into a number of fractions in which the concentration of various trace constituents were above their spectrochemical detection limits; the trace constituents are …
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