Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"elongation complex"”.
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Role of basic and hydrophobic residues in the poliovirus polymerase elongation complex and the structure of a coxsackievirus polymerase elongation complex
… genome, RdRPs must form a stable and processive elongation complex (EC) by binding RNA, incorporating the first templating nucleotide, and undergoing a necessary conformational. Upon completion of these steps that comprise initiation, the newly formed EC is capable of rapidly replicating the …
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RNA replication by poliovirus RNA-dependent RNA polymerase: effects of residue 5 on elongation complex stability and processivity
… change to form a stable RNA-polymerase complex. After the stable RNA-polymerase complex is formed the polymerase undergoes a second conformational change associated with the addition of the first nucleotide to the primer. It is only after these few steps of initiation that the polymerase …
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Adenosine triphosphate is an allosteric inhibitor of coxsackievirus B3 3Dpol
… relative affinities of a stalled coxsackievirus elongation complex for non-cognate nucleotides uncovered a mixed inhibition profile of ATP. Among the six picornavirus species tested, this mode of inhibition seems specific to coxsackievirus B3 (CVB3). Engineered mutations in CVB3 3Dpol, including …
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The bacterial actin-like cell division protein FtsA forms antiparallel double filaments upon binding of FtsN
… by the actin homologue MreB involved in cell elongation. MreB filaments sense curvature and serve as a rudder in the cell elongation complex, ensuring oriented insertion of peptidoglycan around the cell circumference. Following from that I propose that curvature sensing is conserved in FtsA …
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ATM REGULATES THE NF-kB PATHWAY VIA RELA SER 276 PHOSPHORYLATION
… promote nuclear IKKr-ubiquitin association and complex formation with ATM for nuclear export. Activated cytoplasmic ATM is involved in the selective recruitment of the E3-ubiquitin ligase b-TrCP to phospho-IkBa proteosomal degradation. Importantly, ATM binds and activates the catalytic subunit …
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Chromatin Control of the Antiviral Response to Influenza
… modifications serve as scaffolds for protein complexes controlling transcription or co-transcriptional processes, thus impacting gene expression. Histone tail modifications are regulated by multiple, often overlapping pathways in the cell, and as such, present an important regulatory "node" …
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INVESTIGATION OF THE NOVEL ANTITERMINATION MECHANISM OF LOAP, A NUSG SPECIALIZED PARALOG
… plays an important role in the transcription elongation complex across all domains of life. During elongation, the NusG-NTD forms contacts to RNAP while the NusG-CTD dynamically interacts with other proteins, affecting processivity, transcription termination, and in some bacteria, …
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Regulation of Set1-Mediated Methylation of Dam1
… is mediated by Set1 within the COMPASS complex. Methylation requires prior ubiquitination of histone H2BK123 by the E2-E3 ligases Rad6 and Bre1, as well as the Paf1 transcriptional elongation complex. This regulatory pathway exemplifies cross-talk <em>in trans</em> between …
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Identification of Novel Core and Accessory Factors Involved in Nucleotide Excision Repair in Yeast
… only add one by itself. The PAF transcription elongation complex, through facilitating histone modifications, is partially required for dimethylation and fully required for trimethylation of H3K79 by Dot1. We demonstrated that through facilitating these histone modifications, PAF is partially …
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The Role of AF9 and AF9-Mediated Protein Interactions in Hematopoiesis and Leukemogenesis
… AF9 is a member of the pTEFb transcription elongation complex, and interacts with gene regulatory proteins such as AF4/AF5q31, DOT1L, Pc3/CBX8 and BCoR. These interactions are retained in the oncogenic MLL-AF9 fusion protein, and may be required for leukemic transformation.</p><p>Using bone …
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Mechanistic Insights into Direct Interactions that Mediate the Activity of DOT1L Complex in MLL-Rearranged Leukemia
… DOT1L (disruptor of telomeric silencing 1-like) complex. Aberrant histone H3 Lysine 79 (H3K79) methylation catalyzed by DOT1L was shown to be crucial for the maintenance of MLL-rearranged leukemia. DOT1L is the only known H3K79 methyltransferase, and further, a DOT1L inhibitor is currently being …
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A Study of the Therapeutic Potential of AF4 Mimetic Peptides
… are known to aberrantly recruit the super elongation complex (SEC) resulting in constitutive transcription of genes that promote the development of leukemia. Hence, our strategy is to target the SEC as a means of inhibiting MLL leukemia. AF4 and AF9 proteins co-purify with components of the …
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Mechanistic studies on transcription activation via DNA looping in a prokaryotic promoter-enhancer system
… at the promoter by means of DNA looping (closed complex) and induces DNA melting of the promoter DNA by RNAP-sigma54 (open complex). The following aspects of this process were studied: (1) Three different sigma54-specific promoter sequences were analyzed in binding studies and in in vitro …
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Stepwise Reconstitution of RNA Cellular Activity
… of transactivation. This process involves complex RNA-protein interactions between an HIV-1 RNA, the transactivation response element (TAR), and a complex of the viral Tat protein and host super-elongation complex proteins (SEC). I designed library of seventeen TAR mutants and …
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Katalytická funkce DNA dependentních RNA polymeráz
DNA dependentní RNA polymeráza je vysoce organizovaný proteinový komplex, který je zodpovědný za genovou expresi a její regulaci. Vícepodjednotková RNA polymeráza se svými několika katalytickými aktivitami je zodpovědná za transkripci genů do RNA kopií u všech buněčných organismů. Při transkripci …
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Structural and functional consequences of platinum anticancer drug binding to free and nucleosomal DNA
… biological nucleophiles, forms relatively inert complexes with platinum that inactivate cisplatin, and (2) that the major cisplatin-DNA adduct formed is a bifunctional cross-link. These results are in accord with previous studies of cisplatin- DNA binding and reveal that the presence of carbonate …