Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"electrospray ionization mass spectrometry"”.
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Electrospray ionization mass spectrometry for the analysis of proteins and small molecules
… the analysis of proteins and small molecules by Electrospray Ionization Mass Spectrometry (ESI-MS) are discussed. Section I contains an introduction to ESI-MS, including a brief history of mass spectrometry, a discussion of the current theories developed to explain the mechanisms at work during …
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Development and Applications of Liquid Sample Desorption Electrospray Ionization Mass Spectrometry (DESI-MS)
… and the applications of liquid sample desorption electrospray ionization mass spectrometry (DESI-MS), a new ambient ionization method with focus on the direct analysis of liquid samples. A variety of new applications of liquid sample DESI are discussed in details including protein conformation …
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Structural Studies on the Estrogen Receptor Ligand Binding Domain Using Electrospray Ionization Mass Spectrometry
A protocol using LC/ESI-MS and LC/ESI-MS/MS was developed and used successfully to identify the site covalent attachment of the photoaffinity label hexestrol diazirine. The application of tandem MS/MS sequencing permitted the identification of several residues near the C-terminus of the protein.
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Continuous flow fast atom bombardment and electrospray ionization mass spectrometry in natural products research
… has been applied as a liquid chromatography/mass spectrometry (LC/MS) and liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS) interface in natural products research. Initial studies concentrated on characterization of the interface, optimization of operational parameters and …
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Rapid quantification of trypsin inhibitors in food and feed formulations with electrospray ionization mass spectrometry
… (HMTBA) derived oligo lysine with the help of electrospray ionization mass spectrometry (ESI-MS). HMTBA capped oligo lysine was synthesized through a chymotrypsin catalyzed reaction in phosphate buffer. The developed method was favorably compared with the existing method recommended by American …
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Pseudo-Molecular Ion Formation by Aromatic Acids in Negative Ionization Mode Electrospray Ionization Mass Spectrometry
… an artifact common to most analyses performed by electrospray ionization mass spectrometry. These species are non-covalent complexes formed between an analyte of interest and any other components (such as mobile phase, additives, and impurities) present in the ionized sample band. Published …
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A Deuterium Labeling Method for the Characterization of (Chromophoric) Dissolved Organic Matter Using Ultrahigh Resolution Electrospray Ionization Mass Spectrometry
… (NaBD4) and is analyzed via ultrahigh resolution electrospray ionization (ESI) mass spectrometry. Ketone/aldehyde-containing species (at mass m) in the untreated sample are identified by searching the mass spectrum of the reduced sample for peaks corresponding to deuterated derivatives (at mass …
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Mechanistic Insights into Analyte Charge Enhancement using Mass Spectrometry and Molecular Dynamics Simulations
Electrospray ionization mass spectrometry (ESI-MS) is a powerful technique to investigate proteins and many other analytes. However, many fundamental aspects of ESI remain poorly understood. In this thesis, we use a combination of molecular dynamics (MD) simulations and experiments to gain insights …
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Surfactant Assisted Electrospray Ionization Time of Flight Mass Spectrometry of Synthetic Polymers
… to determine chemical composition and structure. Mass spectrometric techniques have greatly enhanced the quantity, as well as the quality, of data that can be used to characterize synthetic polymers. Electrospray Ionization Mass Spectrometry, ESI-MS, is capable of providing a wealth of information …
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Hyphenated techniques for speciation of Se in biological matrices
… species was done by inductively coupled plasma mass spectrometry and electrospray ionization tandem mass spectrometry. The major species identified in yeast-based Se-supplements was Se-Methionine. Furthermore other natural food sources rich in Se were examined: Brazil nuts (containing Se- …
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Biospeciation, Interaction with Biomolecules and Mechanism of Action of Potential Cu and V Drugs
… A, and serum albumin were analyzed by electrospray ionization mass spectrometry (ESI-MS), electron paramagnetic resonance (EPR) spectroscopy, quantum-mechanical (QM) calculations, and molecular docking. These studies identified specific binding sites, competition between ancillary …
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Biophysical Insights into Lipid Oxidation Effects on Membrane Self-Organization and Enzymatic Catalysis Under Extreme Conditions
… glycerol under extreme temperature conditions. Electrospray Ionization Mass Spectrometry (ESI-MS) and spectrophotometric analyses demonstrated the successful formation of high-value products, namely para-nitrophenol (pNP) and glyceryl palmitate, even at elevated tem- peratures. These findings …
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Characterization of Metal Binding Peptides Derived from Copper Trafficking Proteins
… into the environment as the result of the mass generation of oxygen from photosynthetic bacteria roughly 2.7 billion years ago. While it proved to be poisonous to early life on Earth, those that met the evolutionary challenge utilized the metal as a cofactor in enzymes to perform …
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The Synthesis, Characterization, and Properties of Stiff, Hydrophilic, Dendritic, Phenylacetylene Macromolecules
… characterization techniques including NMR, mass spectrometry, elemental analysis, GPC, and HPLC. Careful analysis of an apparently pure dendrimer revealed the presence structures incorporating small quantities of isopropyl esters derived from a trace impurity in the monomer. Conversion of …
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AI-assisted reaction impurity prediction and inverse structure elucidation
… post hoc from analytical data, such as mass spectrometry data – presents a significant challenge. In this work, impurity prediction was established by developing an AI-based reaction predictor that takes as input the main reactants, and reagents, solvents, and impurities in these …
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Evaluation of Ion Transfer Capillary Geometry on Sensitivity of a Desorption Electrospray Ionization and Mass Spectrometry System
… geometry on the sensitivity of the Desorption Electrospray Ionization – Mass Spectrometry (DESI-MS) process. Previous work has shown that heating the ion transfer capillary to 450˚C will improve the resolution of images taken by the DESI-MS owing to increased ion desolvation. This thesis …
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Analyzing A-series gangliosides in neurons following exposure to glutamate
… viability. The purpose of this study is to use electrospray ionization mass spectrometry (ESI-MS) technique and immunohistochemistry to evaluate the temporal changes in the expression profiles of various ganglioside species during the course of neurodegeneration in rat primary cortical neurons …
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In-situ early detection of metal corrosion via "turn-on" fluorescence in "smart" epoxy coatings
… with Fe3+ in a nonaqueous solution by using electrospray ionization mass spectrometry (ESI-MS), the predominant mechanism that FD1 is capable of detecting early metal corrosion is due to its acid-catalyzed hydrolysis to fluorescent protonated Rhodamine B hydrazide, as the consequence of the …
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Enhancing Micro Free Flow Electrophoresis: Detection, Application, And Fabrication
… interfacing incompatible separation modes with electrospray ionization mass spectrometry (ESI-MS) to develop novel multi-attribute analysis techniques for the assessment of therapeutic monoclonal antibodies (mAbs). Lastly, a novel fabrication technique for µFFE was developed to hot emboss µFFE …
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Analytical and metabolic studies of the trypanocidal diamidines
… impurities of diminazene have been identified by electrospray ionization mass spectrometry and characterized by NMR spectroscopy of the synthetic compounds. The developed method has been applied for the quality evaluation of over one hundred generic samples of diminazene obtained from Sub-Saharan …
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