Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"electron cryomicroscopy"”.
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Study of the Yeast Vacuolar-type ATPase by Electron Cryomicroscopy
… of V-ATPases can be analyzed by single particle electron cryomicroscopy (cryo-EM), an imaging technique involving the identification of tens to hundreds of thousands of individual proteins in micrographs. A partially-automated image selection program was developed to increase particle image …
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Investigations of the mechanism of mitochondrial complex I by electron cryomicroscopy
… of metabolism and relaying the liberated electrons through a series of membrane-embedded oxidoreductase proteins known as the electron transport chain (ETC). The first enzyme in the ETC is complex I (NADH:ubiquinone oxidoreductase). Complex I oxidises NADH in the matrix, reduces ubiquinone …
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The physical origins of specimen movement in electron cryomicroscopy and how to eliminate it
Electron cryomicroscopy (cryoEM) is an important imaging technique for determining the atomic structures of biological macromolecules and complexes. Success in determining a structure by cryoEM depends on being able to prepare a thin frozen specimen of the molecules of interest on a small metal …
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Electron cryomicroscopy structures of respiratory supercomplexes from alphaproteobacteria suggest mechanisms to enhance catalysis and to prevent deactivation of respiratory complex I
In mitochondria, the electron transport chain complexes that generate the proton motive force to drive ATP synthesis form oligomeric membrane assemblies known as supercomplexes. Even though they are found throughout nature and several physiological consequences are associated with their depletion, …
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Structural studies of spliceosome assembly and catalysis
… chemical reaction of splicing and resolved by electron cryomicroscopy. The structure of the spliceosome immediately after branching shows how the 5′ splice site is ligated to the branch point adenosine upstream of the 3′ splice site. It reveals the three-dimensional conformation of the …
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INSIGHTS INTO POLYMERISATION OF FTSZ AND OTHER CYTOMOTIVE FILAMENTS
… Here I show using X-ray crystallography and electron cryomicroscopy (cryoEM) that FtsZ switches conformation when it polymerises into filaments. I then show using cryoEM that this conformational switch is likely needed for recognition of filaments by the widely conserved filament …
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Structural Characterization of F-type and V-type Rotary ATPases by Single Particle Electron Cryomicroscpy
… F-type and V-type enzymes using single particle electron cryomicroscopy (cryo-EM), with the aim of better understanding their overall architecture, subunit organization and the mechanism of proton translocation. Our cryo-EM structural analysis on the F-type ATP synthase from Saccharomyces …
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Architecture of eukaryotic mRNA 3' end processing machinery and insights into the mechanism of polyadenylation
… and purified a five-subunit polymerase module. Electron cryomicroscopy analysis resulted in a 3.5 Å resolution structure of Cft1-Pfs2-Yth1, revealing four β propellers in an arrangement reminiscent of other nucleic acid binding complexes. Using biochemical assays, I show that CF IA stimulates …
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Mechanisms of mRNA substrate-selection by the Ccr4-Not deadenylase complex
… of the Ccr4-Not complex was performed using electron cryomicroscopy and single-particle analysis.
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Developing mouse complex I as a model system: structure, function and implications in mitochondrial diseases.
… in the mitochondrial inner membrane, is a major electron entry point to the respiratory chain. It couples the energy released from electron transfer (from NADH to ubiquinone) to the concomitant pumping of protons across the membrane, to generate an electrochemical proton motive force. Mammalian …
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Structural Insights into Noncanonical Mechanisms of Translation
… termination to occur. Using single-particle electron cryomicroscopy (cryo-EM), I obtained four high-resolution structures of nonstop complexes that reveal the mechanism of ArfA-mediated ribosome rescue and have wider implications for understanding canonical termination in bacteria. The aim of …
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Cryo-EM studies of substrate and inhibitor binding to mammalian respiratory complex I
… unknown. In the work presented in this thesis, electron cryomicroscopy (cryo-EM) was used as a primary tool to provide fundamental insight on this mechanism by generating three-dimensional reconstructions of complex I bound to substrates, ligands or inhibitors. Analyses of the structural data …
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Investigating tau propagation in situ
… interactions. To achieve this, I employed electron cryo-tomography (cryo-ET), a powerful technique that allows the visualization of biological samples at molecular resolution in near-native conditions. I wanted to study tau propagation in a disease relevant context. I first focused on human …
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A Geometric Approach for Deciphering Protein Structure from Cryo-EM Volumes
Electron Cryo-Microscopy or cryo-EM is an area that has received much attention in the recent past. Compared to the traditional methods of X-Ray Crystallography and NMR Spectroscopy, cryo-EM can be used to image much larger complexes, in many different conformations, and under a wide range of …