Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"electron cryo-tomography"”.
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A cylindrical specimen holder for electron cryo-tomography
The 'missing wedge' is a long-standing problem in electron tomography, caused by the use of slab-like flat specimens, which increase in thickness when tilted to high angles. Attempts have been made to reduce the undesirable effects caused by the missing wedge, but the problem remains, particularly …
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A cylindrical specimen holder for electron cryo-tomography
The ‘missing wedge’ is a long-standing problem in electron tomography, caused by the use of slab-like flat specimens, which increase in thickness when tilted to high angles. Attempts have been made to reduce the undesirable effects caused by the missing wedge, but the problem remains, particularly …
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Activation of lipid kinase VPS34 by Rab GTPases
… of complex II bound to Rab5 on membranes, using electron cryo-tomography and subtomogram averaging. Complex II is dynamic on membranes with only the UVRAG/Beclin 1 “adaptor arm” permanently contacting the outer leaflet of the lipid bilayer. In contrast, the VPS15/VPS34 “catalytic arm” hovers over …
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Biochemical and structural studies of the FtsZ:FtsA complex and polymerising abilities of the FtsA protein
… in cells when overexpressed, as observed by electron cryo-tomography. Mutants that disrupt polymerisation also showed a significant cell division phenotype in a temperature sensitive FtsA background, demonstrating the importance of filament formation for FtsA’s function in the Z-ring.
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Linking Architecture and Function of Endoplasmic Reticulum - Plasma Membrane Contact Sites
… proteins. By combining correlative light and electron microscopy (CLEM) and electron cryo-tomography (cryo-ET) with yeast genetic techniques, this work addresses unanswered questions about the cellular architecture of ER-PM contacts and how protein organisation in uences their functionality. …
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Reorganization of mitochondrial architecture during apoptosis and mitophagy as investigated by correlative microscopy
… membrane ruptures and appeared to be amorphous. Electron cryo-tomography of vitreous cells was used to discern a higher-order organization to cluster components. In these near- native conditions, Bax clusters appeared to contain a sponge-like meshwork, suggesting that components of the outer …
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Investigating tau propagation in situ
… interactions. To achieve this, I employed electron cryo-tomography (cryo-ET), a powerful technique that allows the visualization of biological samples at molecular resolution in near-native conditions. I wanted to study tau propagation in a disease relevant context. I first focused on human …