Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 86 for “"elastase"”.
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Cellular Uptake of Neutrohpil Elastase Links Inflammation to Adaptive Immunity
… including the serine protease neutrophil elastase (NE). The effect of NE on cancer has been attributed primarily to its ability to degrade the extracellular matrix thereby promoting invasion and metastasis. Recently, it was shown that NE could be taken up by lung cancer cells leading to …
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Imbalance Between Neutrophil Elastase and Elafin Promotes Breast Cancer Growth and Progression
… of the epithelial barrier against neutrophil elastase (NE) activity. The central hypothesis examined in this dissertation was that elafin has tumor suppressive properties in breast cancer. In support of this hypothesis, immunohistochemical (IHC) analysis revealed that elafin was downregulated …
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Doxorubicin-Induced Cardiotoxicity Is Mediated By Neutrophils Through Release of Neutrophil Elastase
… therapy). Furthermore, our data using neutrophil elastase (NE) knock-out mice and the NE inhibitor AZD9668 suggest that neutrophils induce cardiac damage by releasing NE and that inhibiting NE can prevent both acute and late Dox-induced cardiotoxicity. Taken together, this data indicates the …
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The role of elastase as an inflammatory stimulus in chronic obstructive pulmonary disease
… in emphysema where increases in neutrophil elastase concentration results in the destruction of elastin fibres which results in a decrease in lung function. Elastase is also known to contribute to the mucus hypersecretion associated with chronic bronchitis. The proteolytic actions of …
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Modulation of dendritic cells by human neutrophil elastase and its inhibitors in pulmonary inflammation
… to microbial infections and increased neutrophil elastase (NE) in lung secretions. This thesis was designed to test the hypotheses that; (i) excess NE may result in a dysregulation of lung DCs function in pulmonary chronic diseases, and (ii) the natural NE inhibitors in the respiratory system are …
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Characterizing The Source of Neutrophil Elastase and Proteinase 3 Cross-Presentation In B-Cell Acute Lymphoblastic Leukemia
… antigen derived from serine proteases neutrophil elastase (NE) and proteinase 3 (P3). NE and P3 are primarily expressed in cells of myeloid lineage including granulocytes, bone marrow progenitors and myeloid leukemia. Our lab reported that NE and P3 are cross-presented by antigen presenting cells …
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The synthesis and evaluation of valine mimic protio and halo enol lactones as human neutrophil elastase inhibitors
Human neutrophil elastase (HNE) has been found to be responsible for the destruction of lung tissue in emphysema. Based on the primary substrate selectivity of HNE, a series of valine mimic protio and bromo enol lactones were designed as HNE inhibitors. General methods were developed for the …
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Elastase inhibitory, anti-inflammatory, and antioxidant potential of South African plants in a multi-faceted anti-ageing approach
… result in the release of proteases such as elastase which hydrolyze the structural components if the dermis undesirably. This results in the formation of wrinkles. In the present study eleven terrestrial and wetland plants (Aloe ferox, Aloe spicata, Annona senegalensis, Barleria …
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Bioengineering the Expression of Active Recombinant Human Cathepsin G, Enteropeptidase, Neutrophil Elastase, and C-Reactive Protein in Yeast
… human cathepsin G (hCatG) and human neutrophil elastase (HNE) were expressed in P. pastoris and HNE was also expressed in K. lactis. The recombinant variants rhCatG and rHNE, with intact C-terminal extensions, were expressed as fusion proteins with the soluble heme-binding domain of cytochrome …
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Novel findings on cellular trafficking and targeting for granule storage of neutrophil elastase, a multifunctional effector molecule of innate immunity
Neutrophil elastase (NE) has important roles in innate immunity, killing pathogens and controlling the immune response; but how NE is targeted to developing granules is not understood. Therefore, the aim of this thesis was to investigate the sorting of NE. Transfection experiments in a leukemic …
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The synthesis and evaluation of halo and protio enol lactone Pro-Val pseudodipeptides as potential inhibitors of human leukocyte elastase
… of the serine protease human leukocyte elastase (HLE). These lactones are incorporated into a Pro-Val pseudodipeptide in order to enhance binding and specificity towards HLE. The synthesis of these compounds proceeded by the cyclization of N-protected forms of the acetylenic amino acid …
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Enzymatically active microspheres for self-propelled colloidal engines
… biomolecules for self-propulsion. We select elastase as a catalyst and coat it on the surface of polystyrene (PS) particles, and use SucAla<sub>3</sub>-pNA as a substrate to examine the activity of the elastase-coated particles. We exploit biotin-streptavidin chemistry to couple the elastase …
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The Role of the Propeptide and its Residues in Activation and Secretion of Elastase, an M4 Metalloprotease Secreted by Pseudomonas aeruginosa
… but the most abundant and active is elastase (M4 metalloendopeptidase). Elastase (lasB), is first synthesized as a preproenzyme, with a signal peptide, an 18-kDa N-terminal propeptide, and a 33-kDa mature domain. The propeptide functions as an intramolecular chaperone that is required …
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Proteolyse-induzierte Spaltprodukte des Plasminogens
… Peptidbindungen prozessieren können, gehören Elastase aus polymorphnukleären Neutrophilen (PMN) und Metallo-Elastase aus Makrophagen. Ein Spaltprodukt ist Miniplasminogen, das die proteolytische Domäne und den Kringel 5 umfaßt. Die Spaltstelle bei Val441 ist charakterisiert und ein …
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1-Acylamido boronic acids and Difluoroborane analogs of amino acids: Synthesis and biological activity of transition state analogs for Chymotrypsin and Elastase (inhibition, esters)
… of the serine proteases (alpha)-chymotrypsin and elastase. These inhibitors were designed to resemble N-acyl phenylalanine, phenylglycine (as chymotrypsin inhibitors), alanine, valine, and isoleucine (as elastase inhibitors) and have boron sybstituted for the carboxy terminus carbon. Acyl groups …
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NOVEL CINNAMIC ACID-BASED DEHYDROPOLYMERS FOR EMPHYSEMA: IN VITRO AND IN VIVO ASSESSMENT OF THEIR ACTIVITIES
… against oxidative stress, inflammation and elastase, some of the pathogenic mechanisms associated with this disease. Three in vitro inhibitory activity assays for oxidative stress, lung inflammation and neutrophil elastase (NE) were developed and used to identify the most potent triple …
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