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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 128 for “"effector proteins"”.
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Identification and characterization of phosphoinositide-binding Legionella effector proteins
… employing a sizeable artillery of bacterial proteins that subvert multiple host cellular processes without compromising the integrity of the host cell throughout infection (Hubber & Roy, 2010; Robinson & Roy, 2006; Swanson & Isberg, 1995). A specialized secretion system is responsible for …
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Cargo Specific Regulation Of Cytoplasmic Dynein By Effector Proteins
… Previous work has suggested that adaptor proteins provide a mechanism for cargo-specific regulation of motors. During my thesis work, I have investigated the role of mammalian Hook proteins, Hook1 and Hook3, as potential motor adaptors. Using optogenetic and single molecule assays, I found …
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Characterisation of Novel Rab5 Effector Proteins in the Endocytic Pathway
… phosphate PI(3)P a complex network of effectors, including PI3-Kinase (PI3-K), the FYVE-finger proteins EEA1 and Rabenosyn-5 that functionally cooperate in membrane transport. Moreover, Rab5 regulates endocytosis from the apical and basolateral plasma membrane in polarised epithelial …
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Characterization of 16B09 and 2D01 effector proteins in cyst nematodes
Cyst nematodes use stylet-secreted effector proteins to modify a selected host cell into an elaborate feeding cell. The function of the novel effector proteins 16B09 and 2D01 from the soybean cyst nematode (SCN) Heterodera glycines and sugar beet cyst nematode (BCN) H. schachtii was studied. This …
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Localization and Function of Bacterial Type III and IV Effector Proteins
… relay in both space and time. Bacterial effector proteins are virulence factors that are directly secreted from the bacteria into the host cytosol and function to rewire eukaryotic signaling networks to establish an environment for bacterial survival. While much effort has gone into …
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Characterisation of candidate effector proteins from barley pathogen <i>Rhynchosporium commune</i>
… transcripts coding for small secreted fungal proteins called RcCDI1, Rc2 and Rsu3_07158, this last one encoding for a hydrophobic surface binding protein. They are most highly up-regulated early during infection. RcCDI1 and its homologs from different fungal species exhibit PAMP activity. …
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A screen for phosphatidylinositol 3,4-bisphosphate and other 3-phosphoinositide effector proteins
… 3,4- bisphosphate (PtdIns(3,4)P2). Many effector proteins for PtdIns(3,4,5)P3 are well recognised but by contrast, few molecular targets for PtdIns(3,4)P2 have been identified. This study describes a screen to identify PI 3-kinase-responsive proteins that is selective particularly for …
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Biochemical Characterization of IpaH E3 Ubiquitin Ligase Effector Proteins and Their Host Substrates
… its type 3 secretion system (T3SS) to inject effector proteins in the cytoplasm of host cells to manipulate host cells processes. T3SS effectors are able to post translationally modify host proteins to reprogram intracellular signaling pathways, actin dynamics, membrane trafficking, and innate …
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Identification and Characterization of In-planta Expressed Secreted Effector Proteins from Magnaporthe oryzae
… genes, including 851 genes encoding putative effector proteins, were identified. To elucidate the molecular basis of interactions between rice and M. oryzae upon infection, we combined the results from gene expression profiling with high throughput gene cloning, and rapid protoplast transient …
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The Assembly of Pathogenic Signaling Circuits by a Family of Bacterial Secreted Effector Proteins
Bacterial type III secreted effector proteins facilitate Gram-negative bacterial replication, dissemination, and immune evasion in the infected host organism. While much attention has been focused on the cell inhibitory mechanisms of these virulence factors, there is emerging evidence that …
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Delivering Signal-Altering Bacterial Effector Proteins to Mammalian Cells Using Cell-Penetrating Peptide Technology
… signal-altering molecules are known as bacterial effector proteins (BEPs). Bacterial effectors YopJ and VopA have been shown to interfere with specific signaling molecules in the MAPK pathway; effectively inducing apoptosis in mammalian intestinal endothelial cells. In this study, we deliver these …
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Identification and characterisation of phytophthora capsici CRN effector proteins and their targets in planta
… plant pathogens secrete a large amount of effector molecules into the host plant. These effectors are thought to aid the infection processes by fending off the plant’s defence and modifying its transcriptional machinery to benefit the pathogen. This is true for Phytophthora capsici an …
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Identification and functional characterization of RXLR effector proteins that are conserved between downy mildew pathogens and Phytophthora species
Diverse pathogens secrete effector proteins into plant cells to manipulate host cellular processes. The genome of Hyaloperonospora arabidopsidis (Hpa), the causative agent of downy mildew of Arabidopsis, contains at least 134 candidate RXLR effector genes. These genes contain an RXLR motif required …
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Unveiling and exploiting <i>Phytophthora capsici</i> effectors and their host targets, with an emphasis on CRN effector proteins
… for which virulence is aided by the secretion of effectors, including cytoplasmic effectors from the CRN (CRinkling and Necrosis) family.<br/><br/>CRN effectors were first described in oomycetes by their capacity to induce host cell death. Nevertheless, despite recent efforts aiming to elucidate …
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Identification of Candidate Effector Proteins from Fusarium graminearum during Infection of Arabidopsis thaliana Using Proximity-Dependant Biotin Identification (BioID)
… head blight (FHB) in cereal crops. Identifying proteins that are secreted from pathogens to overcome plant defenses and cause disease, collectively known as effectors, can reveal new targets for fungicides or other control measures.Proximity-dependent biotin identification (BioID) was used to …
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Elucidating essential roles of oomycee effector proteins in immune suppression and in targeting hormonal pathways in the host plant
Effector proteins are exported to the interior of host cells by numerous plant pathogens. Effector proteins have been well characterized in bacteria. However, the mechanisms through which these effectors promote virulence are largely unknown. Bioinformatic analysis of genome sequences from oomycete …
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Novel use of a cell-penetrating peptide-adaptor system to characterize Akirin constructs and investigate activity of bacterial effector proteins
… to which mediates the delivery of exogenous proteins into cells and prevents endosomal entrapment. Calmodulin (CaM) is capable of binding to the calmodulin binding site (CBS) of cargo only in the presence of calcium. TAT-CaM can be used to characterize Akirin constructs and investigate the …
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How Oomycete and Fungal Effectors Enter Host Cells and Promote Infection
… pathogenesis through the use of small secreted proteins known as effector proteins. A large subset of effector proteins is able to translocate into host cells and target plant defense pathways. P. sojae Avr1b is able to suppress cell death triggered by BAX and hydrogen peroxide. The W-domain of …
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A Systems Biology Approach to Study Type III and Type IV Bacterial Effector Properties
… between Type III and Type IV bacterial effector proteins and host signal transduction enzymes is a critical interface that, in many cases, determines the outcome of infectious disease. While many pathogenic strategies, such as evasion of phagolysosomal fusion, have been identified as …
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