Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 144 for “"ectopic expression"”.
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Manipulation of second messengers by ectopic expression of receptors in transgenic Drosophila
… in principal cells. This study has validated ectopic transgene expression as a generic technology with potential beyond Malpighian tubules: in principle, such transgenes can be expressed specifically in any population of cells that can be delineated by a GAL4 driver line.
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Progenitor cells in the postnatal central nervous system, characterization and genetic manipulation
… fate in the striatum. Our result showed that ectopic expression of the transcription factors Islet1 and Neurogenin2 directed the SVZ cells progeny to a new fate in the striatum and external capsule. Once removed to the culture dish they expressed the neuronal marker ?-III-tubulin. Thus, …
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Characterizing the Role of TLX1 in T-cell Acute Lymphoblastic Leukemia
… hematological tumors caused by the aberrant expression of a subset of transcription factors (TFs) in T-cell progenitors1–3 . The identification of diverse sets of secondary mutations in T-ALL tumors suggests a complex process of clonal selection through which oncogenic TF-expressing …
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The Role of Pax3 in Neuronal Differentiation of the Ophthalmic (OpV) Trigeminal Placode and Neural Tube during Chicken Embryonic Development
… ophthalmic (opV) placode development. While Pax3 expression coincides with opV neuron specification, the function of Pax3 in these cells has not previously been investigated. Splotch mutant mice (which lack Pax3) have a reduced trigeminal ganglion; however it is not clear whether this reduction is …
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Integrin Alpha Subunit Ratios, Cytoplasmic Domains, and Growth Factor Synergy Regulate Muscle Proliferation, Differentiation, and Signaling
… in muscle differentiation was addressed by ectopic expression of integrin alpha subunits in primary quail skeletal muscle. Ectopic expression of either the human or $\alpha$5 or the chicken $\alpha$6 subunit produced contrasting phenotypes. The $\alpha$5 transfected myoblasts remain in the …
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Direct Reprogramming Of Fibroblasts Into Muscle Or Neural Lineages By Using Single Transcription Factor With Or Without Myod Transactivation Domain
… this myogenic reprogramming event. In addition, ectopic expression of NeuroD1 and MDA-NeuroD1 is able to induce neurogenic genes in MEFs, suggesting the partial neurogenic conversion of MEFs. Furthermore, we also showed that the ectopic expression of NeuroD1 but not MDA-NeuroD1 in myoblasts could …
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Ehrlichia chaffeensis E3 Ligase TRP120 Ubiquitinates Tumor Suppressor FBW7 for Degradation to Promote Infection
… FBW7 FBOX and WD40 domains were demonstrated by ectopic expression and co-immunoprecipitation. Although FBW7 gene expression increased during E. chaffeensis infection, FBW7 levels significantly decreased (>70%) by 72 h post infection. Moreover, an iRNA knockdown of FBW7 coincided with increased …
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Blimp-1 Regulates Terminal Differentiation of T Cells
… particularly its minor splice variant. Ectopic expression of Blimp-1 by retroviral transduction neither dramatically altered secretion of IFN-ã or IL-4 nor did it induce the ability to suppress as regulatory T cells. However, induction of Blimp-1 resulted in not only a significant …
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The B55α/PP2A Holoenzyme in Cell Cycle Exit, Maturation/Differentiation, and Cancer
… without an increase in B55α protein expression in RCS cells. Reciprocal solution-based mass-spectrometry analysis identified the PP2A/B55α complex as a major component of a subset of p107 complexes, which also contain E2F/DPs, DREAM subunits and cyclin/CDK complexes. p107 is one of …
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Role of lncRNA in cancer development and progression
… and colorectal cancer cells and tissues, PICART1 expression was decreased. Ectopic expression of the PICART1 suppressed growth, proliferation, migration, and invasion of MCF7, MDA-MB-231 and HCT116 cells whereas silencing of PICART1 stimulated the cell growth and migration. In these cells, the …
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miR-322/503 Cluster Drives Cardiac Differentiation by Inhibiting CUG-binding Protein 1
… stem cells (mESCs) differentiation; (2) induced ectopic expression of miR-322/503 to mimic the natural course in mESCs led to α-actinin expression and significant increases of cardiac transcription factors (Tbx5, Mef2C, Nkx2-5 and α-MHC); and (3) inhibitors of miR-322 and miR-503 significantly …
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Understanding molecular events during barley aphid interactions
… the Late Embryogenesis Abundant gene 14 (LEA14). Ectopic expression of two barley thionins in Nicotiana benthamiana reduced host susceptibility to M. persicae, potentially reflecting a role in defence against aphids. We have generated barley mutant lines, using CRISPR-Cas9 technology, to …
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Homologous recombination and directed differentiation in medaka ES cells: Development of vector systems
… and Dmc1 in this cellular event were studied by ectopic expression. Then we took directed melanocyte differentiation as a model to enrich transgenic differentiating cells. For this, a bicistronic plasmid was constructed that enables the transgenic medaka embryonic stem cell to differentiate into …
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Derivation of next generation research models of Parkinson’s disease
… large scale within a short timeframe through the ectopic expression of transcription factors (TFs). In this study, we validated a significant finding: ectopic expression of ATOH1 alone is sufficient to generate Tyrosine hydroxylase (TH)-positive neurons. Additionally, we identified several TF …
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Role of Xenopus Pitx3 during early development.
… level to human and murine Pitx3. Early neurula expression is restricted to lateral prechordal mesoderm, anterior paraxial mesoderm, a crescent of anterior sensorial ectoderm, and a discrete spot which is fated to form Rathke's pouch. xPitx3 is expressed throughout lens induction in the …
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Modeling Alzheimer's Disease Using Cellular Reprogramming Technologies
… demonstrate that cell-fate can be converted by ectopic expression of defined transcription factors: induced pluripotent stem (iPS) cell technology and induced neuronal (iN) cell technology. These recent advances in cell reprogramming strategies have great potential utility for patient-specific …
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A role for the Spemann organizer gene, Goosecoid, in tumor metastasis
… in a majority of human breast tumors. Ectopic expression of Goosecoid in human breast cells generated invasion-associated cellular changes, including an epithelial-mesenchymal transition. TGF-03 signaling, known to promote metastasis, induced Goosecoid expression in human breast cells. …
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THE METHIONINE CYCLE AS A NOVEL THERAPEUTIC TARGET IN NPM1C DRIVEN ACUTE MYELOID LEUKEMIA
… data generated in our lab have shown that ectopic expression of NPM1c in Murine Embryonic Fibroblasts (MEFs) leads to an unbalanced methionine-cycle with decreased ratio between S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH), key metabolites of the methionine cycle. Decreased …
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Využitie transgénnych rastlín pri štúdiu funkcie rastlinných génov
… RNA interference that inactivates or reduces the expression of the individual genes studied. We can also inactivate whether to specifically modify the function of a particular selected gene using site-specific endonucleases, which include meganucleases, zinc finger nucleases, TALEN (transcription …
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