Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"early endosomes"”.
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Kinetic analysis of endosome processing : maturation of early endosomes and vesicular traffic to lysosomes
… in the endocytic pathway, in particular, early endosome processing and delivery to the lysosomes. Two models for endosome processing have previously been proposed in the literature, namely the maturation and vesicular traffic models. The general consensus has been an early phase of …
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Vps1, a Novel Recycling Factor For the Traffic of Early Endosome to Late Golgi
… budding yeast, there are recycling traffics from early and late endosomal compartments to the late Golgi. Here, I examined a possible role for Vps1, a large GTPase, in the recycling traffic of GFP-Snc1 from early endosomes to the late Golgi. In the absence of Vps1, I observed an aberrant …
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Characterisation of Novel Rab5 Effector Proteins in the Endocytic Pathway
… mechanism of endocytosis and transport in the early stages of the endocytic pathways. Rab5 is a key regulator of clathrin-mediated endocytosis, but in addition, localises to several distinct endocytic carriers including phagosomes and pinocytic vesicles. On early endosomes, Rab5 coordinates …
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Proteomic analysis of the Mycobacterium avium-containing phagosome membrane
… of these glycoconjugates as compared to early endosomes with which the phagosomes continuously fuse and exchange membrane molecules... The aim of this study is to identify the depleted / possibly enriched cell surface derived glycoproteins on the phagosome membrane of M.av-containing …
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Rab-domain dynamics in endocytic membrane trafficking
… to mediate trafficking between Golgi/TGN and endosomes instead.In order to address the dynamics of Rab-domains, fluorescently tagged Rab-GTPases were imaged during cargo transport reactions in living cells. Herefore high-speed/long-term imaging procedures and novel computational image analysis …
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Identification and Characterization of Effectors/Binding Molecules for the Small GTPase Rab15
… receptor internalization into early endosomes. Here internalized receptors are\r\nsorted for degradation in lysosomes, direct recycling back to the cell surface or indirect\r\nrecycling via a second recycling compartment called the pericentriolar recycling\r\nendosome. Rab …
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Deciphering The Role of Huntingtin In Endosomal Functions
… endosomal pathways. Upon endocytosis into early endosomes regulated by Rab5, the endocytosed cargo can either be recycled back to the plasma membrane (via Rab4/Rab11) or degraded through the endosomal-lysosomal pathway (via Rab7). Disturbances in normal Rab functions could lead to abnormal …
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Optimization of organelle fractionation methods for quantitative analysis of gene delivery trafficking kinetics
… primary rat hepatocyte lysate into nuclear, early endosomal, late endosomal/lysosomal, and cytoplasmic fractions. We found that according to the Horseradish Peroxidase assay, late endosomes and lysosomes consistently localize to fractions 11-13 and early endosomes in fractions 18 to 21. There …
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Characterization of the Human Adenovirus 19a E3/49K Protein
… Interestingly, one Ad transcription unit, the early transcription unit 3 (E3), appears to encode exclusively proteins with immuno-modulatory functions. Recently, a novel open reading frame, E3/49K, was identified in the E3 region of the epidemic keratoconjunctivitis causing serotype Ad19a. The …
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Quo vadis: quantitative evaluation of intracellular kinetics of siRNA delivered by poly-and-lipoplexes
… membranes. PEI-based particles internalize into early endosomes and gain access to cytosol primarily from early endosomes 14 minutes after the internalization. On the contrary, DOTAP/cholesterol liposomes primarily gain access to cytosol through direct fusion with plasma membranes, and increasing …
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Exploring the Impact of Endothelial Bone Morphogenetic Protein Receptor 2 Loss on Phosphoinositide and Cytoskeletal Assembly Dynamics and the Pathobiology of Pulmonary Arterial Hypertension
… abnormalities in TGN structures, enlarged early endosomes, and an altered cytoskeleton compared to controls. Additionally, siBMPR2 cells exhibited accelerated ERK phosphorylation kinetics upon VEGF stimulation, similar to siCIRC and siLn HPAECs. Further, siBMPR2 HPAECs demonstrated an …
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Biodistribution of Modified Extracellular Vesicles In Normal and Tumor-Bearing Animal Models
… in diameter from 30-150 nm. They are formed by early budding into early endosomes then released by the cell from late endosome. The content of EVs includes proteins, mRNA, miRNA, and DNA derived from the original cell where they were released. Due to the membrane structure of EVs, which resemble …
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Identification of a Novel Link between the Motor Proteins Dynein and Kinesin-1
… studies in filamentous fungi, have shown that early endosomes (EEs) are specifically recognized and moved by dynein from the MT plus-ends at the hyphal tips to more distal cellular compartments. Interestingly, the targeting of dynein to MT plus-ends, is dependent on kinesin-1, and its …
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Somatostatin Receptor 2 Regulation By C-Terminal Motifs and Pdz Domain Proteins
… We determined that sst2 traffics from early endosomes to late endosomes and then on to the trans-golgi network (TGN). Trafficking from late endosomes to the TGN is dependent upon an intact C-terminal tail and the retromer complex of endosomal coat proteins. Additionally, removing either …
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The Interplay of Signal Transduction and Membrane Traffic of the Macrophage Colony Stimulating Factor Receptor (MCSFR) Controls Macrophage Growth
… Following these two steps, the Rab5 positive early endosomes containing MCSFR fuse with nascent macropinosomes and the MCSFR is subsequently transported into the lumen of the macropinsomes and degraded. Pharmacological disruption of macropinocytosis impairs receptor degradation. Entry into …
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Activation of lipid kinase VPS34 by Rab GTPases
… PI(3)P production of complex II specifically on early endosomes.
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Survival Of Mycobacterium Avium Subspecies Paratuberculosis In The Pol
… of viable map and m. Tuberculosis with the early endosomal marker, transferrin receptor occurred with an estimated five fold higher frequency than did association with the late lysosomal marker, lamp-1, as compared to live e. Coli, and all dead bacterial species. Using differential live/dead …
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The Contribution of APP gene dosage to the molecular phenotypes of Alzheimer's disease in human trisomy 21 neurons
… locus duplication which is fully penetrant for early onset familial Alzheimer's disease. However, not everyone with trisomy 21 develops clinical symptoms of Alzheimer's disease. In this thesis, human PSCs were differentiated into cortical neurons to study the cellular phenotypes associated with …
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Platelet Dense Granules Mature Within Late Stages Of Megakaryocyte Differentiation By Cargo Delivery
… G1ME2, labeling by mepacrine overlapped nearly completely with labeling by LysoTracker. Fluorescent puncta that labeled uniquely for mepacrine were first evident in G1ME2-derived proplatelets, suggesting that DGs undergo a maturation step that initiates in the final stages of MK …
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Molecular Analysis of Hepatitis C Virus Envelope Glycoprotein E2 Binding and Entry
… cellular membranes following acidification of endosomes. All known HCV-receptor interactions are mediated by HCV envelope protein 2 (E2) but the manner in which E2 coordinates interactions with multiple entry receptors and cell surface co-factors in order to promote viral entry are only just …
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