Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"early differentiation"”.
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Gcn5 Impacts Fgf Signaling At Multiple Levels and Activates C-Myc Target Genes During Early Differentiation of Embryoid Bodies
… assay (embryoid body formation, EB) to model the early development stages that proceed gastrulation, and report that GCN5, a histone acetyltransferase (HAT) essential for embryonic development, is required for proper expression of multiple genes encoding components of the FGF signaling pathway, …
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In Vitro Assessment of Bone and Cartilage Stimulating Peptides
… on osteoprogenitor proliferation, osteogenic differentiation, cell culture mineralization and cell migration. BCSPs appeared to enhance the bone growth in the previous study using an animal model; however, the cellular and molecular mechanisms of BCSPs performance remain unclear. In this …
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Contributions to Statistical Problems Related to Microarray Data
… expressed genes. Second, we study the cellular differentiation process and proposed a statistical test for detecting early differentiation genes. Third, we further proposed a model-based method for the cellular differentiation problem.
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The Role of Nitric Oxide and Peroxynitrite in Human Embryonic Stem Cell Differentiation
… have been hindered by a lack of knowledge of the differentiation mechanisms. It is not clear what kind of message has to be delivered to hESC to start differentiation, and what process leads to the selected cell differentiation directions. At this stage of research, it is impossible to direct the …
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In vitro generation and programming of human CD8+ memory T cells
… priming, and found memory populations with an early differentiation status could be generated with superior effector function, establishing an expansion protocol with potential implications for adoptive immunotherapy. With this priming regime, a short duration of IL-2 supplementation was found …
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The regulation of mouse embryonic stem cell differentiation by Nrf2
Embryonic stem (ES) cell maintenance and differentiation are dynamic processes controlled by various intrinsic and extrinsic factors. Identifying these factors will enhance the understanding about developmental process and improve the application of stem cells in clinic. Previous studies highlight …
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SPINAL MUSCULAR ATROPHY ORGANOIDS REVEAL DEVELOPMENTAL DEFECTS RESCUED BY ANTISENSE OLIGONUCLEOTIDES TREATMENT
… organoids (SCOs) to recapitulate human-specific early neurodevelopmental features. Single-cell transcriptomics revealed a pervasive transcriptional alteration in multiple cell populations, along with drastic changes in motor neuron gene expression. Morphological analyses detected early …
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Kinetics of notch signaling during osteoclastogenesis
… pathway known to regulate cell proliferation, differentiation and survival in many cell types including the differentiation of osteoclasts from bone marrow macrophages (BMMs). Osteoclastogenesis is initiated in BMMs by receptor activator of nuclear factor kappa-B ligand (RANKL) in the presence …
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Quantification of gene expression in single cells
… can partly explain the divergent inactivation. Early differentiation of human embryonic stem cells involves the transcription factors Pou5f1, Nanog and Sox2. We quantified their expression in single stem cells and observed that they are not correlated with each other. Instead Pou5f1 correlates …
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Cell-to-cell variability and culture conditions during self-renewal reversibly affect subsequent differentiation of mouse embryonic stem cells
… embryonic stem cell (ESC) pluripotency and differentiation. This may be a source of heterogeneity resulting in mixtures of cell types in differentiated populations despite efforts to control the differentiation conditions and the use of a clonal starting population. In addition, this …
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Investigating the role of NADPH oxidase 4 (NOX4) in differentiation and function of induced pluripotent stem cell–derived endothelial cells
… of NADPH oxidase-dependent redox signalling in differentiation of mouse and human iPS-ECs, and provide evidence implicating ROS derived from the NOX4 isoform during the differentiation process, which is likely to be important when considering their therapeutic potential. Our findings show that …
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Understanding and Targeting Clearance of Persistent HPV Infections
… of a real-time cell cycle indicator (FUCCI) and early differentiation marker (KRT10); or the competition between differently fluorescently labelled infected versus uninfected cells. Here, it is presented that HPV16E6 expressing keratinocytes exhibited increased proliferation, delayed contact …
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Regulation and patterning of cell differentiation and pluripotency
… strategies in regulation and patterning of cell differentiation and pluripotency, by pursuing three related research projects: 1. Reversible immortalization of cardiomyocytes to enable their proliferation necessary for obtaining large cell numbers 2. Patterning of the delivery of Doxycycline …
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Activin/Nodal Signalling Controls the Epigenome and Epitranscriptome of Human Pluripotent Stem Cells
… signalling pathways regulating pluripotency and differentiation, our mechanistic understanding of the downstream regulations is still incomplete. Moreover, studies aimed at clarifying these aspects are severely impeded by the lack of efficient methods to conditionally modulate gene expression in …
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A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… Studies that have examined mESC pluripotency and early differentiation dynamics have suggested that mESCs transition towards lineage specification through a transient state of primed pluripotency that resembles that of EpiSC pluripotency. However, this hypothesis has not been methodically …
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The cell-secreted microenvironment : shaping embryonic stem cell self-renewal and differentiation
… as cells enter a primed, epiblast-like state of early differentiation when microfluidic perfusion is used to deplete soluble cell-secreted signals. We show that this phenotypic change can be used to our advantage for directed differentiation, and further demonstrate that remodeling the endogenous …
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Genome-Wide Profiling Unveils Criticial Functions of P53 In Human Embryonic Stem Cells
… roles are believed to coincide, as p53 promotes differentiation in response to DNA damage, but this is unexplored in hESCs.</p> <p>To determine the biological roles of p53, specifically in hESCs, we mapped genome-wide chromatin interactions of p53 by chromatin immunoprecipitation and massively …
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조골세포 사멸 및 분화에서 GSK3b의 역할
… known to induce osteoporosis via a decrease of differentiation and apoptosis of osteoblasts and osteocytes in part. However, the detailed mechanism of GC-induced osteoporosis has not been clearly determined. This study showed that dexamethasone (Dex) induced apoptosis of MC3T3-E1 osteoblasts …
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Regulation of human satellite cells in vitro via inflammatory cytokines and myogenic transcription factors across proliferation and differentiation
… of satellite cell activation, proliferation, and differentiation, the underlying mechanisms regulating this process is not fully understood. Recent characterization of cytokines secreted by skeletal muscle in an endocrine type fashion has led to discoveries of inflammatory cytokines influencing …
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Functional and Transcriptional Heterogeneity of the Human Haematopoietic Stem Cell Pool at Steady-State and Under Inflammation
… to a dynamic continuum. Understanding the early differentiation trajectories of HSCs, and how environment and molecular factors can modify them, is vital in furthering our insight into human haematopoiesis in health and disease. Here I combined index sorting, single cell functional assays …
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