Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"eIF2a"”.
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THE ADENOVIRUS E1B-55K AND E4ORF6 PROTEINS LIMIT PKR-MEDIATED EIF2A PHOSPHORYLATION DURING THE LATE PHASE OF INFECTION
During a productive adenovirus infection, the E1B-55K and E4orf6 proteins promote efficient late viral gene expression. The results presented in this dissertation reveal that the E1B-55K and E4orf6 proteins facilitate late viral protein synthesis by limiting phosphorylation of eIF2α by the …
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Mechanistic study on the therapeutic strategy to enhance glioma cell death through inhibition of proteasome and lysosome
… lysosome. In part I, we investigate the role of eIF2a in BZ-induced cell death. We found that ER-derived dilation preceded bortezomib induced cell death. eIF2α was initially phosphorylated but it was dephosphorylated in parallel with the ER dilation. Overexpression of eIF2a WT markedly increased …
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Unveiling the Intercompartmental Signaling Axis: Mitochondrial to ER Stress Response (MERSR) and Its Impact on Proteostasis
… is enhanced through the upregulation of the PERK-eIF2a signaling pathway, increasing phosphorylation of eIF2a and improving the ER’s capacity to manage greater proteostasis load. MERSR activation in both poly-glutamine (poly-Q) and amyloid-beta (Aß) C. elegans disease models also leads to …
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Multilayered regulation of translation: roles of rRNA modifications, alternative initiation factors, and RNA-binding proteins
… analyses of the alternative initiation factor eIF2A reveal its contributions to non-canonical initiation events. Loss of eIF2A reduces ribosome occupancy at the start codon region of specific genes, suggesting a role in stabilizing pre-initiation complexes or facilitating start codon …
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New Roles for the Integrated Stress Response in Cancer and Proteostasis
… the alternative translation initiation factor, EIF2A, which we previously found to be critical for oncogenesis. Utilizing dual luciferase reporters for alternative translation (Atf4-firefly) and housekeeping translation (HBB-renilla) we performed a high throughput screen and have identified …
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Modulation of Stress Granules formation: Role of mGlu5 receptor and FMRP and implications for pathophysiology of Fragile X Syndrome
… components and RNA binding proteins, like eIF2a and FMRP. Several data also indicate that some of the FXS symptoms are a consequence of a defect in group-I metabotropic glutamate receptor, namely mGlu5; pharmacological blockade of mGlu5 receptors provide a therapeutic target in FXS. mGlu5 …
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A maternal low-protein diet during gestation induces hepatic autophagy gene expression only in the female offspring Sprague-Dawley rats
… expression was consistent with the activated eIF2a/ATF4 pathway in female offspring. Furthermore, we found the maternal low-protein diet induces ATF4 and C/EBP homology protein (CHOP) binding at the regions of specific autophagy-related genes in the liver of female offspring through …
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RNA-binding proteins mediate anti-inflammatory regulation of vascular disease
… significantly reduced the formation of SGs and eIF2a phosphorylation, suggesting a relationship between inflammation and SG formation in vascular cells. Reduction of SG component G3BP1 by siRNA knockdown significantly reduced stress granule formation and inflammatory gene abundance in hVSMC. …
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Evolutionary conservation and characterization of the metazoan amino acid response
… conserved phosphorylation site in the protein eIF2a, the signature ISR effector, which allowed me to use a mammalian antibody to identify and characterize the ISR in the basal metazoan, Nematostella vectensis, revealing that the core components of the mammalian ISR were present over 550 million …
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The role of cellular signalling pathways and translation initiation factors in Herpesvirus infection
… instead, increased the association of inactive eIF2a with initiation complexes. At these relatively low concentrations a potent suppression of mRNA translation was achieved yet tolerable to cells over prolonged periods. Critically, inhibition of translation resulted in suppression of both lytic …
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Molecular Effects of the Nampt Inhibitor FK866 on Leukemia Cells
… cascade and of the major initiation factor EIF2A, impairing protein synthesis. Furthermore, FK866-induced stress reduced the levels of the anti-apoptotic protein MCL1 and impacted on the endoplasmic reticulum homeostasis. In addition, we established and characterized an FK866-resistant model …
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Studies on the interaction between porcine reproductive and respiratory syndrome virus and its natural host cell
… by triggering the activation of NF-κB or eIF2a, respectively. It worth noting that the modulatory activities became critical when the PRRSV-infected cells were super-exposed to secondary stimulation of poly(I:C) or LPS. In which case exacerbated inflammation response in the lung will …
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Immunometabolism of methyl donors by dairy cattle during periods of negative nutrient balance and heat stress
… Protein abundance of phosphorylated (p) EEF2 and EIF2A decreased with enhanced choline supply potentially helping to maintain or increase protein synthesis during NNB. Overall, the enhanced supply of choline during NNB increases flux through the Met cycle to regenerate Met and reduce liver TAG …