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Showing 1 to 20 of 20 for “"dynactin"”.
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Visualisation of dynein complexes in vitro and inside cells
… is activated by binding to a co-factor called dynactin. How dynactin binding activates dynein is unknown. How multiple dynein/dynactin complexes are arranged on cargo for long range transport in cells is also not clear. To understand this, I used cryo-electron microscopy (EM) combined with …
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Investigating the cytoskeleton and microtubule-based transport with in situ cryo-electron tomography
… Cytoplasmic dynein, in combination with dynactin and activating adaptors, is a microtubule-based motor that drives long-range transport of many cargo types towards microtubule minus ends. Structural and cellular work to date has revealed how dynein-dynactin complexes form and arrange on …
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Unraveling the multifunctionality of human Spindly
… to be required for recruitment of the dynein/dynactin motor complex to kinetochores thus to promote chromosome alignment and mitotic progression. Kinetochore recruitment of the dynein/dynactin motor complex is crucial for maturation of kinetochore-microtubule attachments and for silencing the …
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Genetic analysis of the maintenance of neuronal morphology in Drosophila melanogaster
… basally into the brain. Disruption of the Dynactin complex, which activates the minus-end-directed microtubule motor protein Dynein, causes mislocalization of photoreceptor nuclei basally, even into the optic stalk and brain. Photoreceptors in animals mutant for the Dynactin subunit Glued …
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Novel Regulatory Mechanisms of Cytoplasmic Dynein: A Role for the Complex Base
… many recruitment and regulatory factors, with dynactin and NudE/L-Lis1 being the most ubiquitous and well described. Additionally we have recently gained detailed, high-resolution structures of the dynein motor domain and models for dynein stepping and mechanochemistry based on single molecule …
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The structure of the cytoplasmic dynein tail
… Dynein, together with its large co-factor dynactin, is responsible for the vast majority of traffic towards the centre of the cell. The largest subunit of the dynein complex is called the dynein heavy chain (DHC). The DHC includes a C-terminal motor domain, which converts ATP hydrolysis …
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CHARACTERIZATION OF THE ROLES OF MUSCLE-SYNTHESIZED BRAIN-DERIVED NEUROTROPHIC FACTOR AND PRESYNAPTIC TYROSINE RECEPTOR KINASE B IN MOTOR NEURON AXONAL TRANSPORT
… of numerous adaptor proteins, including dynactin and JIP3, which help bind cargo to motor proteins. Disruption of retrograde transport is a hallmark of several neuromuscular diseases, including SBMA, Huntington’s disease, and ALS. The roles of BDNF and TrkB are not well-characterized in …
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The role of ZW10 and its binding partners in chromosome segregation
… a non-redundant role for CLIP- 170, dynein/dynactin, LIS 1 and TOG I in chromosome congression to the metaphase plate. Selective depletion of dynein/dynactin and CLIP- 170 from mitotic kinetochores by RNAi of ZW 10, uncovered a novel role for these proteins in the initial …
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High-throughput evaluation of protein folding conditions and expression constructs for structural genomics
… eines humanen Proteins, der p22 Untereinheit von Dynactin, welche in E. coli in Inclusion Bodies exprimiert wird. So wie es sich bei vielen Proteinen darstellt, war auch für p22 Dynactin kein biologischer Nachweistest vorhanden, um den Erfolg des Faltungsexperimentes zu messen. Die Löslichkeit des …
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Messenger Ribonucleoprotein (Mrnp) Complexes: Proteomic and Functional Analysis
… Furthermore, while KLC is dispensable, dynactin is required for dFMRP-RNP transport, indicating a novel attachment of kinesin-1 to dFMRP-RNP.
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Identification of binding protein with kinesin18B
… coli(APC) and p150glued, a component of the dynactin complex. EB1 interacts with kinesin18B and has been localized to clusters near the distal ends of microtubules. Kinesin18B has been shown to move along microtubules and has been located at the plasma membrane and nuclear. The interaction …
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Functional characterization of the novel centrosomal protein Nlp (ninein-like protein)
… to associate with the cytoplasmic dynein-dynactin complex, a molecular motor complex primarly involved in the maintainance of Golgi architecture. We propose that the excess of Nlp and ninein could induce the disruption of the Golgi apparatus by sequestering the dynein-dynactin complexes. …
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THE ROLE OF SKELETAL MUSCLE-SYNTHESIZED BRAIN-DERIVED NEUROTROPHIC FACTOR IN RETROGRADE TRANSPORT ALONG MOTORNEURON AXONS
… of NF-H-P and the motor associated protein, dynactin 1, immediately distal to the ligation site in muscle-BDNF deficient mice at 120d, but not at 30d. These results indicate that loss of muscle-synthesized BDNF leads to disruptions in retrograde transport in 120d animals. To investigate …
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Identifying targets of the MAST kinase Drop out through genetic and proteomic analysis in Drosophila melanogaster
… dop mutants and mutants of the Dynein and Dynactin complexes. Furthermore, Dop is identified as a regulator of the Hedgehog signalling pathway, and is required in signal transduction.
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Cargo Specific Regulation Of Cytoplasmic Dynein By Effector Proteins
… that Hook proteins interact with both dynein and dynactin, to effectively activate dynein motility, inducing longer run lengths and higher velocities than the previously characterized dynein activator, BICD2. In addition, I found that complex formation requires the N-terminal domain of Hook …
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The Role of Spindle Orientation in Epidermal Development and Homeostasis
… LGN, Nuclear mitotic apparatus (NuMA) and dynein/dynactin, plays a key role in establishing proper spindle orientation during ACDs. Two of these proteins, NuMA and dynein, interact directly with astral microtubules (MTs) that emanate from the mitotic spindle. While the contribution of these …
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A Genome-Wide Screen on Modifiers of Tau-Induced Neurodegeneration Using RNAi-Mediated Gene Silencing in Drosophila
… beteiligt sind, darunter Bestandteile des Dynein/Dynactin-Komplexes, der für den retrograden Mikrotubuli-basierten Transport verantwortlich ist. Experimente zur Aufklärung der funktionalen Zusammenhänge zwischen retrogradem Transport und Tau-vermittelter Toxizität weisen darauf hin, dass in diesem …
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Regulation of Bidirectional Microtubule-Dependent Organelle Transport in Xenopus Laevis Melanophores
… transport were utilized to determine that dynactin, a large multi-subunit protein complex, acts as a receptor for both kinesin-2 and dynein on the melanosome surface, and may serve to coordinate the activity of these motors during regulated transport. Further exploration into the mechanisms …
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Investigating dynein-based transport of intracellular pathogens
… to promote the formation of motile dynein-dynactin complexes. In Chapter 4, I present the results of a collaborative project in which I leveraged the cell-based assays I developed during my PhD to study the function of the dynein regulator LIS1. Finally, in Chapter 5, I investigate the …
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Involvement of a specific ubiquitin ligase in the assembly of the dynein motor in the filamentous fungus Neurospora crassa
… chains, light intermediate chains, and dynactin to form a motor complex of several megadaltons. The last two thirds of the dynein heavy chains contain the main motor unit required for force production, while the N-terminal tail region of the dynein heavy chain along, with other dynein …