Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"dual-color"”.
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Cross-talk-free dual-color fluorescence cross-correlation spectroscopy for high-throughtput screening
… toward the design and construction of a two-color FCCS system, which employed two spectrally separate fluorophores, Cy3 (λabs = 532 nm, λem= 560 nm) and IRD800 (λabs = 780 nm, λem= 810 nm). The system provided negligible color cross-talk (cross-excitation and/or cross-emission) and/or …
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Sunlight readability and luminance characteristics of light-emitting diode push button switches.
… under ambient sunlight, legend uniformity, and dual-color uniformity. Three of the four manufacturers have not developed LED push button switches that meet lighting quality standards established with incandescent technology.
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Electro-optical systems and neural-inspired processing: A system architecture and technology analysis with applications to law enforcement small arms fire indication systems
… sensors and neural-inspired processing. Dual-color, high-rate, imaging, and high-performance indication systems are all evaluated. A platform-level design decomposition that starts with the customer needs and finishes with the sensor and processor requirements is performed; the …
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Analysis of Interphase Chromatin Motion in HeLa Cells
… which modify chromatin mobility. Using dual color imaging of flourescently tagged chromatin and proliferating cell nuclear antigen (PCNA) were followed through interphase. Chromatin motion was modelled as a two dimensional random walk. Reduction in chromatin mobility was observed during …
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Studying viral-like particles using fluorescence fluctuation spectroscopy
… to the characterization of two proteins using dual color labeling. We explore the application of two-dimensional photon counting histogram (2D-PCH) analysis to determine the average copy number of two proteins in VLPs. Additionally, we present a new method called dual-color intensity fraction …
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Quantitative Fluorescence Studies in Living Cells: Extending Fluorescence Fluctuation Spectroscopy to Peripheral Membrane Proteins
… was further used in combination with dual-color competition studies to determine the lipid specificity of protein binding. Finally, we applied z-scan FFS to provide insight into the early assembly steps of the HTLV-1 retrovirus.
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OmpD but not OmpC is involved in adherence of Salmonella enterica serovar Typhimurium to human cells
… ratios for 1 h at 37 C. Single and dual fluorescence of samples was analyzed by flow cytometry to obtain mean fluorescence intensity (MFI). The MFI values of cells with adherent wild-type and mutant bacteria were compared to determine adherence levels. MFI values were used to …
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Visualizing inhibitory and excitatory synapse dynamics In vivo
… To address this issue, we utilized triple-color two photon microscopy to examine inhibitory and excitatory synapses across time with daily imaging. We found that the majority of dynamic spines at these intervals lacked a mature excitatory synapse as indicated by the absence of PSD-95. …
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Feto-Maternal Exosome Characterization, Trafficking and Function During Pregnancy and Parturition
… in mice, we developed a model which utilizes a dual color fluorescent transgenic Cre reporter mouse. Using this model, we show feto-maternal crosstalk via exosomes occurs during pregnancy and can produce functional changes on both sides of the placenta. Further research into the mechanism of …
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Unsupervised tracking and automated analysis of multi-population neural activity under anesthesia
… multisession datasets. By optimizing and using dual-color imaging, we recorded simultaneously the activity of excitatory neurons and subsets of inhibitory interneurons at different stages of anesthesia. The automated analysis pipeline allowed us to study the behavior of the same individual …
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Advanced Fluorescence Correlation Techniques to Study Membrane Dynamics
… scanning FCS and to quantify binding with dual color scanning FCS. Spectral crosstalk can be avoided effectively by using alternating excitation. Using this method we were able to perform measurements in systems previously not accessible with FCS, such as yeast cell membranes or membranes …
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Analyse der Wechselwirkungen von Proteinen des Interleukin-6-Signalwegs in einzelnen Zellen
… exchange rapidly with cytosolic Jaks. Applying a dual-color FRAP approach it turned out that immobilization of gp130-CFP by a pair of monoclonal antibodies led to a corresponding immobilization of cotransfected Jak1-YFP. The conclusion from these findings is that Jak1, once bound to a gp130 …
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Applications of two focus fluorescence correlation spectroscopy in colloid and polymer science
… obtained on colloidal systems by a new method, dual focus Fluorescence Correlation Spectroscopy (2fFCS), which permits investigations of comparable precision to established methods. 2fFCS is insensitive to many optical and photo-physical effects. An external length scale is introduced into the …
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Lokalisation, Regulation und Interaktionen muriner DNA-Replikationsproteine
… PCNA-Proteinen in murinen L-Fibroblasten durch Dual-Color-Immunfluoreszenz- (IF-) Studien untersucht. Dabei konnte gezeigt werden, dass die Proteine des ORC, des MCM-Komplexes und der Elongations-Maschinerie Positionen für drei verschiedene mechanistische Teil-Prozesse markieren, die an der …
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The mechanisms of reliable coding in mouse visual cortex
… pyramidal neurons. Using a novel combination of dual-color calcium imaging and optogenetic manipulation, I have discovered that the SST->PV inhibitory circuit plays a crucial role in modulating pyramidal cell reliability. In particular, by transiently suppressing PV neurons, SST neurons are able …
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Bispektrale Detektion und optische Nichtlinearitäten in Quantentopf-Infrarot-Photodetektoren
… ermöglichte die Realisierung einer Dual-Band-Bildfeldmatrix mit 384 x 288 Bildpunkten, welche erstmals thermische Auflösungen unterhalb von 30 mK (f/2-Optik, 6.8ms Integrationszeit) in beiden Detektionsbereichen erreicht. <br> <br>Ferner werden Detektoren beschrieben, die auf eine …
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Investigating spatial distribution and dynamics of membrane proteins in polymer-tethered lipid bilayer systems using single molecule-sensitive imaging techniques
… domains. Moreover, using a newly developed dual color FCS and PCH assay, the composition of uPAR and integrin within complexes was determined for the first time. From the perspective of cell mechanics, the characterization of the dynamic assembly of membrane proteins during formation of cell …
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Single molecule studies of branched polymer dynamics in non-dilute solutions
… to directly observe the dynamics of individual polymer chains, thereby bridging the gap in understanding between molecular and bulk-scale properties. Recent single molecule studies have explored the dynamics of linear and ring polymers in dilute and non-dilute solutions, and well-defined …