Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 119 for “"dsRNA"”.
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Effect of dsRNA-containing and dsRNA-free hypovirulent isolates of Fusarium oxysporum on severity of Fusarium seedling disease of Essex soybean
… isolates were tested for the presence of dsRNA. The presence of dsRNA was not associated with hypovirulence in <I>F. oxysporum</I> since some hypovirulent isolates contained dsRNA while other hypovirulent isolates did not. Furthermore, of six dsRNA-containing <I>F. oxysporum</I> isolates, …
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The Role of MDA5 and TLR3 in Response to dsRNA and Viral Infection
… the course of infection, many viruses produce dsRNA that can be recognized by two major arms of the innate immune system: the toll-like receptors: TLR) and the Rig-I-like receptors: RLR). Among the TLRs, TLR3 binds dsRNA within the endosomal compartment and initiates signaling through its …
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Characterization of Ebola virus VP35-dsRNA binding for drug development against Ebola virus disease.
… plates using a fluorescent labeled 30mer dsRNA was validated, showing a VP35 Kd value for dsRNA binding around 4 nM, comparable to the one previously reported, and a Z'-factor equal to 0.69, that indicate a good assay. The use of this biochemical assay to screen the ability of plant …
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In-vitro analysis of microfilariae phenotypic responses induced by small molecules and dsRNA targeting receptor pathways
… in microfilariae to small molecules as well as dsRNA molecules targeting genes involved in receptor pathways essential for nematode function. Utilizing the video analysis software called the Worminator, we quantified changes in movement upon exposure to agonists and antagonists acting on various …
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Determining the mechanism of double-stranded RNA-induced cell death in ovarian cancer.
… Within tumors pathogen-derived ligands, such as dsRNA, can activate pattern recognition receptors (PRRs) that are capable of inducing apoptosis. In this dissertation we have found that in ovarian cancer cell lines (DOV-13, SKOV-3, CAOV-3, and OVCAR-3), dsRNA treatment alters cell survival. When …
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IDENTIFICATION OF FACTORS INVOLVED IN DOUBLE STRANDED RNA-INDUCED CELL DEATH THROUGH GENOME-WIDE CRISPR SCREENS
Double-stranded RNAs (dsRNAs) are potent immunostimulatory nucleic acids of viral origin but also physiologically produced by mammalian cells. Increased levels of dsRNAs, due to viral infection or failure of endogenous repression, trigger an immune response that leads to cytokines production or …
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The Mechanism of Double-Stranded RNA Response in Neurospora
… cells, recognition of double-stranded RNA (dsRNA) by the enzyme Dicer initiates the RNA interference (RNAi) pathway, resulting in post-transcriptional gene silencing. Argonaute proteins play a critical role in this conserved pathway, which is present in protists, fungi, plants and animals. …
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Molecular Mechanism of MDA5 RNA Sensing in Innate Immunity and Disease Pathogenesis
… combating viral infections. Double-stranded RNA (dsRNA) is a potent proinflammatory signal, and its recognition by MDA5 and its coregulatory factor, LGP2, is essential for initiating antiviral responses. This study aimed to investigate the structure and function of MDA5 and LGP2. I showed the …
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Reading Between the Strands: Deciphering Double Stranded RNA-Based Immune Responses in Mosquito-Virus Interactions
… molecular patterns, with double-stranded RNA (dsRNA), a replication intermediate of many viruses, serving as a key immunostimulatory signal. While RNA interference (RNAi) remains the most well-characterized antiviral pathway triggered by dsRNA, accumulating evidence suggests that mosquitoes …
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Isolation and characterization of dengue virus membrane-associated replication complexes from Aedes aegypti
… may play in shielding DENV double-stranded RNA (dsRNA) from RNAi. Membrane isolation techniques and immunofluorescent staining techniques for dsRNA identification were developed to isolate and characterize membrane-associated replication complexes in DENV-infected mosquito cell culture and Ae. …
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Mobile RNA reveal differential requirements for gene silencing in C. elegans at single-cell resolution
Delivery of double-stranded RNA (dsRNA) into animals can silence genes of matching sequence in diverse cell types through mechanisms that have been collectively called RNA interference. In the worm C. elegans, such organism-wide silencing relies on the transport of dsRNA to most cells and requires …
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Double-stranded RNA uptake in Caenorhabditis elegans
dsRNA uptake from the environment is a common phenomenon in many invertebrates and can be harnessed for acquiring pest control. In the nematode Caenorhabditis elegans, a transmembrane protein named systemic RNAi deficient-2 (sid-2) is required for dsRNA uptake from the environment through the …
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Mechanisms of toxicity in cell models of FSHD
… cells accumulate large double-stranded RNA (dsRNA) foci within their nuclei, corresponding with induction of the pro-apoptotic PKR dsRNA response pathway. By high- throughput sequencing RNAs immunoprecipitated with anti-dsRNA antibodies I determined that DUX4-induced dsRNAs appeared to …
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The antitumor activity of tumor-targeted RNA replicase-based plasmid DNA
… have been numerous attempts to utilize synthetic dsRNA to control tumor growth in animal models and clinical trials. Recently, it has become clear that intracellular dsRNA is more effective than extracellular dsRNA in promoting apoptosis and orchestrating adaptive immune response. To overcome the …
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Studies on the interaction between porcine reproductive and respiratory syndrome virus and its natural host cell
… following the cells’ exposure to synthetic dsRNA. Interestingly, there was no corresponding impairment of the activation of either interferon regulatory factor 3 (IRF3) or signal transducer and activator of transcription 1 (STAT1), or of the transcription of the IFN-α, IFN-b, or IRF7 genes. …
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The Function and Mechanism of RNA Interference in Neurospora
… RNAi is triggered by double stranded RNA (dsRNA), which is processed by Dicer to siRNA. siRNA is loaded onto RNA-induced silencing complex (RISC), in which an Argonaute family protein, guided by a siRNA, mediates the cleavage of homologous RNAs. In the filamentous fungus Neurospora, we show …
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SYSTEMIC AND TRANSGENERATIONAL REGULATION OF GENE EXPRESSION BY SMALL RNAS IN C. ELEGANS
… regulation). Double- stranded RNA (dsRNA) introduced to an animal can silence a gene of matching sequence within that animal and this silencing can persist in progeny suggesting that RNA has the potential to transfer gene-specific regulatory information. Using RNA silencing in C. …
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Single molecule pull down of double strand RNA binding proteins
… regions, or known as double-strand RNAs (dsRNAs). The class of proteins responsible for processing dsRNAs is termed double-stranded RNA binding proteins (dsRBP). In recent decades, an increasing number of reports have shown the role of dsRBP-dsRNA interaction as core strategy in various …
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The Biogenesis of Small Interfering RNA in Neurospora Crassa
… RNAi is initiated by the production of dsRNA, which is processed by Dicer to produce small interfering RNA (siRNA). In the filamentous fungus, Neurospora crassa, two types of siRNA have been characterized. One is involved in transgene-induced silencing, termed quelling; the other type is …
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Evidence for RNA interference as an antiviral immune response to alphavirus infection in the mosquitoes Anopheles gambiae and Aedes aegypti
… gambiae cells using a dual-luciferase assay and dsRNA knockdown techniques. These experiments identified the Dcr2, Ago2, and Ago3 genes from An. gambiae as genes involved in the RNAi pathway. To test the hypothesis that the RNAi pathway is a part of the mosquitoe's innate defenses against virus …
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