Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 154 for “"dsDNA"”.
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Structure of the GP16 ATPase from the φ29 dsDNA packaging motor
… bacteriophage φ29 packages double-stranded DNA (dsDNA) into a preformed viral shell, or procapsid, and serves as a model system for studying genome packaging in eukaryotic dsDNA viruses such as poxviruses, herpesviruses and adenoviruses. Encapsidation of bacteriophage φ29 DNA is driven by a …
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Structural and Biochemical Examination of Aberrant dsDNA Metabolism in TREX1-associated Autoimmunity
… DNA during apoptosis. Accumulation of self–dsDNA antibodies accompanies dysfunctional TREX1 and triggers the innate immune response. TREX1–associated dominant disease phenotypes correlate to an inability of TREX1 heterodimers containing one dominant–mutant protomer to efficiently …
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Studies on anti-dsDNA Antibodies and other potential biomarkers in Systemic Lupus Erythematosus
… SLE patients. Autoantibodies, in particular anti-dsDNA antibodies, are considered hallmarks of SLE, but the ideal biomarker for SLE is still missing. In this thesis, I summarize the state of the art about biomarkers for SLE and present four original papers, resulting from two different …
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Mechanisms and regulation of dsDNA break repair in the Sulfolobus genus of thermophilic archaea
… is essential for all life. Double stranded DNA (dsDNA) breaks are especially toxic as both antiparallel strands of DNA are severed. The most high fidelity mechanism available to repair this damage is homologous recombination, a mechanism that uses homology from the sister chromatid to replace any …
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Towards molecular autopsies: Internal validation of the Qubit 1 X dsDNA HS Assay Kit
… study was to internally validate the Qubit™ 1X dsDNA HS Assay Kit on the Qubit™ 4 Fluorometer as part of a molecular autopsy workflow for forensic applications. The Qubit™ 1X dsDNA HS Assay workflow was optimised and then used to assess the DNA concentration of control Lambda DNA, extracted DNA …
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NZB/NZW F1-Derived Monoclonal Anti-Dna Autoantibodies: Dual-Specificity and Immune Complex Formation
… with the primary pathogenic antibodies being dsDNA specific. Recent studies had identified a subpopulation of anti-DNA autoantibodies that possessed dual-specificity and appeared to form very avid immune complexes. The dual-specific autoantibodies were derived from the NZB/NZW F1 murine model …
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Covalent end-immobilization of oligonucleotides onto solid surfaces
… psoralen crosslinkers covalently immobilized the dsDNA molecules to the oligo surface. The covalently end-attached dsDNA formed brush-like structures where the dsDNA strand could react under conditions resembling the natural solution-state found in vivo. This method minimized the possibility of …
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Revealing SMC5/6's Dynamic Behavior on Diverse DNA Topologies Through Single-Molecule Methods
… DNA substrates, including double-stranded DNA (dsDNA), stretches of single-stranded DNA (ssDNA), chromatinized DNA, forked/junction DNA, and recombination intermediates. In this thesis, our approach was to utilize correlative single-molecule fluorescence and force microscopy, which combined …
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Investigating the binding mode of azide labelled derivatives of Hoechst 33258 by NMR, UV-Vis and IR spectroscopy
… to develop a method for studying the dynamics of dsDNA: small molecule ligand association and dissociation through 2D-IR spectroscopy. The strategy employed was to synthesise azide-bearing derivatives of the archetypal minor groove binding ligand, Hoechst 33258, with the azide acting as a reporter …
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Effect of Naturally Occurring DNA Modifications on DNA Structure and Packaging
In eukaryotes, the genomic double-stranded DNA (dsDNA) coils around histones to form nucleosomes. Arrays of these nucleosomes bundle together to generate chromatin. Most DNA-related processes require interactions between chromatin-protected DNA and cellular machinery. Access of cell machinery to …
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Jednoduchý elektrochemický DNA biosenzor pro detekci poškození DNA způsobeného UV zářením
… and low molecular weight doble-stranded DNA (dsDNA) from salmon sperm. Biosensor was prepared using adsorption of dsDNA on a surface of the UTGE and then used to detect UV-induced DNA damage. Effects of UV radiation were investigated using a combination of several electrochemical technics: …
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Mechanisms of Microbial DNA Sensing by the AIM2 Inflammasome and Structural Study of Polynucleotydyl Transferase1 (pnt1) from Arabidopsis Thaliana
… can sense foreign cytosolic double-stranded DNA (dsDNA) through the dsDNA binding HIN-200 domain at the C-terminus. Once dsDNA is bound to HIN-200 domain, AIM2 can activate the AIM2 inflammasome, resulting in maturation of pro-interleukin-1? by activation of caspase-1. To investigate the mechanism …
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Exogenously-introduced Homing Endonucleases Catalyze Double-stranded DNA Breaks in Aedes aegypti
… elements which introduce double-stranded DNA (dsDNA) breaks in a sequence-specific manner. In this study, we aimed to validate a somatic assay to detect recombinant homing endonuclease (rHE)-induced dsDNA breaks in both cultured cells and adult female Ae. aegypti. While the cell culture-based …
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Biochemical and Biophysical Characterization of Replicative Helicase Loading by <i>Vibrio cholerae</i> DciA, Crystal Structure of a Monoclonal Antibody Bound to a C-Terminal Fragment of EBNA-1 from Epstein Barr Virus
… that react against double stranded DNA (dsDNA) as being a disease driver for Systematic Lupus Erythematosus (SLE). Studies in mice show that immune responses against the Epstein Barr virus Nuclear Antigen 1 (EBNA-1) protein can produce antibodies that also react with dsDNA. Furthermore, …
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Qualify Microplate Reader’s Specification and Establish the Acceptance Criteria for Modified PicoGreen Kit in Measuring cell-free DNA Concentration in Clinical Laboratory
… of extraction yield various concentrations of dsDNA. Our laboratory develops the use of a modified PicoGreen kits and Biotek’s microplate reader to measure cfDNA concentration. This assay is being qualified to allow the laboratory to assess the yields of dsDNA after extraction method. …
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Pathological role of double-stranded DNA antibodies in multiple sclerosis.
… the aetiology remains largely unanswered. Anti-dsDNA antibodies have been found intrathecally and bordering lesions in multiple sclerosis patients and in view of their known pathogenity in lupus nephritis the aim of this project was to further investigate their role in multiple sclerosis. Using …
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Pathological role of double-stranded DNA antibodies in multiple sclerosis.
… the aetiology remains largely unanswered. Anti-dsDNA antibodies have been found intrathecally and bordering lesions in multiple sclerosis patients and in view of their known pathogenity in lupus nephritis the aim of this project was to further investigate their role in multiple sclerosis. Using …
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Using Synthetic Nanopores for Single -Molecule Analyses: Detecting SNPs, Trapping DNA Molecules, and the Prospects for Sequencing DNA
… measuring the transition from double-stranded (dsDNA) to single-stranded DNA (ssDNA), called the helix-coil transition, which is vital to biology. The force required to dissociate base pairs depends on whether the DNA is unzipped by pulling parallel to the bases or stretched by pulling …
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Single molecule fret study on the mechanism of RecA mediated strand exchange
… increments with destabilization of the incoming dsDNA and concomitant pairing with the reference ssDNA. Our model for strand exchange links structural models of RecA to its catalytic function. Next, we investigated the mechanism of RecA mediated homology search. Using tools with high …
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Expression and characterization of recombinant human NEIL3 from Escherichia coli
… on 8-oxoG. In contrast, in double-stranded DNA (dsDNA), activity on 5-OHU:G and TG:A was only weakly observed with nominal activity on 8-oxoG:C. The oxidised bases were then placed at three different sites in a model replication fork, at position -4 in ssDNA, at the fork junction (the last …
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